Flow cytometry of the Side Population: Tips & tricks

Cellular Oncology

Volumn 28, Issue 1-2, 2006, Pages 37-53

  Sales Pardo, Irene ^a   Avendaño, Ariadna ^a   Martinez Muñoz, Vanessa ^a   García Escarp, Marta ^b   Celis, Raquel ^a   Whittle, Phil ^c   Barquinero, Jordi ^b   Domingo, Joan Carles ^a   Marin, Pedro ^a   Petriz, Jordi ^a,d  

^a UNIVERSITY OF BARCELONA   (Spain)

^b Transfusion Center and Tissue Bank   (Spain)

^c AGILENT TECHNOLOGIES   (United States)

^d HOSPITAL UNIVERSITARI VALL D HEBRON   (Spain)

Author keywords

CD34 negative; Flow cytometry; Hoechst 33342; Side Population; Stem cells

Indexed keywords

AMMONIUM CHLORIDE; HOE 33342;

ANIMAL CELL; ARTICLE; CELL ISOLATION; CELL LABELING; CELL POPULATION; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; HEMATOPOIETIC CELL; HEMOLYSIS; LASER; MOUSE; NONHUMAN; OPTICS; PRIORITY JOURNAL; STEM CELL; ULTRAVIOLET RADIATION;

ANIMALS; ANTIGENS, CD34; BONE MARROW CELLS; CELL SEPARATION; FETAL BLOOD; FLOW CYTOMETRY; HUMANS; MICE; MICE, INBRED C57BL; QUALITY CONTROL; STEM CELLS;

EID: 33646368112     PISSN: 15705870     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (12)

1. A. Asakura, P. Seale, A. Girgis-Gabardo and M.A. Rudnicki, Myogenic specification of side population cells in skeletal muscle, J. Cell Biol. 159(1) (2002), 123-134.
2. H.M. Blau, T.R. Brazelton and J.M. Weimann, The evolving concept of a stem cell: entity or function?, Cell 105(7) (2001), 829-841.
3. M.A. Goodell, K. Brose, G. Paradis, A.S. Conner and R.C. Mulligan, Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo, J. Exp. Med. 183(4) (1996), 1797-1806.
4. M.A. Goodell, M. Rosenzweig, H. Kim, D.F. Marks, M. DeMaria, G. Paradis, S.A. Grupp, C.A. Sieff, R.C. Mulligan and R.P. Johnson, Dye efflux studies suggest that hematopoietic stem cells expressing low or undetectable levels of CD34 antigen exist in multiple species, Nat. Med. 3(12) (1997), 1337-1345.
5. K.A. Jackson, S.M. Majka, H. Wang, J. Pocius, C.J. Hartley, M.W. Majesky, M.L. Entman, L.H. Michael, K.K. Hirschi and M.A. Goodell, Regeneration of ischemic cardiac muscle and vascular endothelium by adult stem cells, J. Clin. Invest. 107(11) (2001), 1395-1402.
6. A. Lechner, C.A. Leech, E.J. Abraham, A.L. Nolan and J.F. Habener, Nestin-positive progenitor cells derived from adult human pancreatic islets of Langerhans contain Side Population (SP) cells defined by expression of the ABCG2 (BCRP1) ATP-binding cassette transporter, Biochem. Biophys. Res. Commun. 293(2) (2002), 670-674.
7. F. Montanaro, K. Liadaki, J. Volinski, A. Flint and L.M. Kunkel, Skeletal muscle engraftment potential of adult mouse skin side population cells, Proc. Natl. Acad. Sci. USA 100(16) (2003), 9336-9341.
8. A. Murayama, Y. Matsuzaki, A. Kawaguchi, T. Shimazaki and H. Okano, Flow cytometric analysis of neural stem cells in the developing and adult mouse brain, J. Neurosci. Res. 69(6) (2002), 837-847.
9. R.W. Storms, M.A. Goodell, A. Fisher, R.C. Mulligan and C. Smith, Hoechst dye efflux reveals a novel CD7(+)CD34(-) lymphoid progenitor in human umbilical cord blood, Blood 96(6) (2000), 2125-2133.
10. W.G. Telford and E.G. Frolova, Discrimination of the Hoechst side population in mouse bone marrow with violet and near-ultraviolet laser diodes, Cytometry 57A(1) (2004), 45-52.
11. N. Uchida, T. Fujisaki, A.C. Eaves and C.J. Eaves, Transplantable hematopoietic stem cells in human fetal liver have a CD34(+) side population (SP) phenotype, J. Clin. Invest. 108 (7) (2001), 1071-1077.
12. S. Zhou, J.D. Schuetz, K.D. Bunting, A.M. Colapietro, J. Sampath, J.J. Morris, I. Lagutina, G.C. Grosveld, M. Osawa, H. Nakauchi and B.P. Sorrentino, The ABC transporter Bcrp1/ABCG2 is expressed in a wide variety of stem cells and is a molecular determinant of the side-population phenotype, Nat. Med. 7(9) (2001), 1028-1034.