Mutational analysis of the chemoreceptor-coupling domain of the Escherichia coli chemotaxis signaling kinase CheA

Journal of Bacteriology

Volumn 188, Issue 9, 2006, Pages 3299-3307

  Zhao, Jinshi ^a   Parkinson, John S ^a  

^a UNIVERSITY OF UTAH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEA KINASE; CHEW PROTEIN; MUTANT PROTEIN; PROTEIN HISTIDINE KINASE;

ARTICLE; AUTOPHOSPHORYLATION; CARBOXY TERMINAL SEQUENCE; CHEMORECEPTOR; CHEMOTAXIS; DIMERIZATION; ESCHERICHIA COLI; MISSENSE MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY;

BACTERIAL PROTEINS; BINDING SITES; CHEMORECEPTORS; CHEMOTAXIS; DNA MUTATIONAL ANALYSIS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MEMBRANE PROTEINS; MODELS, MOLECULAR; MUTATION, MISSENSE; PHOSPHORYLATION; PROTEIN STRUCTURE, TERTIARY; STRUCTURE-ACTIVITY RELATIONSHIP;

ESCHERICHIA COLI;

EID: 33646249421     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.188.9.3299-3307.2006     Document Type: Article

References (52)

1. Ames, P., and J. S. Parkinson. 1994. Constitutively signaling fragments of Tsr, the Escherichia coli serine chemoreceptor. J. Bacteriol. 176:6340-6348.
2. Ames, P., C. A. Studdert, R. H. Reiser, and J. S. Parkinson. 2002. Collaborative signaling by mixed chemoreceptor teams in Escherichia coli. Proc. Natl. Acad. Sci. USA 99:7060-7065.
3. Ames, P., Y. A. Yu, and J. S. Parkinson. 1996. Methylation segments are not required for chemotactic signalling by cytoplasmic fragments of Tsr, the methyl-accepting serine chemoreceptor of Escherichia coli. Mol. Microbiol. 19:737-746.
4. Bibikov, S. I., R. Biran, K. E. Rudd, and J. S. Parkinson. 1997. A signal transducer for aerotaxis in Escherichia coli. J. Bacteriol. 179:4075-4079.
5. Bilwes, A. M., L. A. Alex, B. R. Crane, and M. I. Simon. 1999. Structure of CheA, a signal-transducing histidine kinase. Cell 96:131-141.
6. Bolivar, F., R. Rodriguez, P. J. Greene, M. C. Betlach, H. L. Heyneker, and H. W. Boyer. 1977. Construction and characterization of new cloning vehicles. Gene 2:95-113.
7. Borkovich, K. A., and M. I. Simon. 1991. Coupling of receptor function to phosphate-transfer reactions in bacterial chemotaxis. Methods Enzymol. 200:205-214.
8. Borkovich, K. A., and M. I. Simon. 1990. The dynamics of protein phosphorylation in bacterial chemotaxis. Cell 63:1339-1348.
9. Bornhorst, J. A., and J. J. Falke. 2001. Evidence that both ligand binding and covalent adaptation drive a two-state equilibrium in the aspartate receptor signaling complex. J. Gen. Physiol. 118:693-710.
10. Boukhvalova, M., R. VanBruggen, and R. C. Stewart. 2002. CheA kinase and chemoreceptor interaction surfaces on CheW. J. Biol. Chem. 277:23596-23603.
11. Boukhvalova, M. S., F. W. Dahlquist, and R. C. Stewart. 2002. CheW binding interactions with CheA and Tar: importance for chemotaxis signaling in Escherichia coli. J. Biol. Chem. 277:22251-22259.
12. Bourret, R. B., J. Davagnino, and M. I. Simon. 1993. The carboxy-terminal portion of the CheA kinase mediates regulation of autophosphorylation by transducer and CheW. J. Bacteriol. 175:2097-2101.
13. Chen, J. 1992. Genetic studies of transmembrane and intracellular signaling by a bacterial chemoreceptor. Ph.D. thesis. University of Utah, Salt Lake City.
14. Cox, E. C., and D. L. Horner. 1986. DNA sequence and coding properties of mutD(dnaQ) a dominant Escherichia coli mutator gene. J. Mol. Biol. 190:113-117.
15. Garzón, A., and J. S. Parkinson. 1996. Chemotactic signaling by the P1 phosphorylation domain liberated from the CheA histidine kinase of Escherichia coli. J. Bacteriol. 178:6752-6758.
16. Gegner, J. A., and F. W. Dahlquist. 1991. Signal transduction in bacteria: CheW forms a reversible complex with the protein kinase CheA. Proc. Natl. Acad. Sci. USA 88:750-754.
17. Gegner, J. A., D. R. Graham, A. F. Roth, and F. W. Dahlquist. 1992. Assembly of an MCP receptor, CheW, and kinase CheA complex in the bacterial chemotaxis signal transduction pathway. Cell 70:975-982.
18. Griswold, I. J., and F. W. Dahlquist. 2002. The dynamic behavior of CheW from Thermotoga maritima in solution, as determined by nuclear magnetic resonance: implications for potential protein-protein interaction sites. Biophys. Chem. 101-102:359-373.
19. Griswold, I. J., H. Zhou, M. Matison, R. V. Swanson, L. P. McIntosh, M. I. Simon, and F. W. Dahlquist. 2002. The solution structure and interactions of CheW from Thermotoga maritima. Nat. Struct. Biol. 9:121-125.
20. Hess, J. F., R. B. Bourret, and M. I. Simon. 1991. Phosphorylation assays for proteins of the two-component regulatory system controlling chemotaxis in Escherichia coli. Methods Enzymol. 200:188-204.
21. Jahreis, K., T. B. Morrison, A. Garzon, and J. S. Parkinson. 2004. Chemotactic signaling by an Escherichia coli CheA mutant that lacks the binding domain for phosphoacceptor partners. J. Bacteriol. 186:2664-2672.
22. Kishan, K. V., G. Scita, W. T. Wong, P. P. Di Fiore, and M. E. Newcomer. 1997. The SH3 domain of Eps8 exists as a novel intertwined dimer. Nat. Struct. Biol. 4:739-743.
23. Kott, L., E. H. Braswell, A. L. Shrout, and R. M. Weis. 2004. Distributed subunit interactions in CheA contribute to dimer stability: a sedimentation equilibrium study. Biochim. Biophys. Acta 1696:131-140.
24. Laemmli, U. K. 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227:680-685.
25. Levit, M. N., and J. B. Stock. 2002. Receptor methylation controls the magnitude of stimulus-response coupling in bacterial chemotaxis. J. Biol. Chem. 277:36760-36765.
26. Li, G., and R. M. Weis. 2000. Covalent modification regulates ligand binding to receptor complexes in the chemosensory system of Escherichia coli. Cell 100:357-365.
27. Li, M., and G. L. Hazelbauer. 2004. Cellular stoichiometry of the components of the chemotaxis signaling complex. J. Bacteriol. 186:3687-3694.
28. Liu, J. 1990. Molecular genetics of the chemotactic signaling pathway in Escherichia coli. Ph.D. thesis. University of Utah, Salt Lake City.
29. Liu, Y., M. Levit, R. Lurz, M. G. Surette, and J. B. Stock. 1997. Receptor-mediated protein kinase activation and the mechanism of transmembrane signaling in bacterial chemotaxis. EMBO J. 16:7231-7240.
30. Matsumura, P., J. J. Rydel, R. Linzmeier, and D. Vacante. 1984. Over-expression and sequence of the Escherichia coli cheY gene and biochemical activities of the CheY protein. J. Bacteriol. 160:36-41.
31. McNally, D. F., and P. Matsumura. 1991. Bacterial chemotaxis signaling complexes: formation of a CheA/CheW complex enhances autophosphorylation and affinity for CheY. Proc. Natl. Acad. Sci. USA 88:6269-6273.
32. Morrison, T. B., and J. S. Parkinson. 1997. A fragment liberated from the Escherichia coli CheA kinase that blocks stimulatory, but not inhibitory, chemoreceptor signaling. J. Bacteriol. 179:5543-5550.
33. Ninfa, E. G., A. Stock, S. Mowbray, and J. Stock. 1991. Reconstitution of the bacterial chemotaxis signal transduction system from purified components. J. Biol. Chem. 266:9764-9770.
34. Park, S., P. P. Borbat, G. Gonzalez-Bonet, J. Bhatagar, A. M. Pollard, J. H. Freed, A. M. Bilwes, and B. R. Crane. Nat. Struct. Mol. Biol., in press.
35. Parkinson, J. S. 1976. cheA, cheB, and cheC genes of Escherichia coli and their role in chemotaxis. J. Bacteriol. 126:758-770.
36. Parkinson, J. S. 1978. Complementation analysis and deletion mapping of Escherichia coli mutants defective in chemotaxis. J. Bacteriol. 135:45-53.
37. Parkinson, J. S., and S. E. Houts. 1982. Isolation and behavior of Escherichia coli deletion mutants lacking chemotaxis functions. J. Bacteriol. 151:106-113.
38. Rasmussen, R. P. 1998. Biochemical phenotypes of mutations in the chemotactic signaling complex of E. coli. Ph.D. thesis. University of Utah, Salt Lake City.
39. Sanatinia, H., E. C. Kofoid, T. B. Morrison, and J. S. Parkinson. 1995. The smaller of two overlapping cheA gene products is not essential for chemotaxis in Escherichia coli. J. Bacteriol. 177:2713-2720.
40. Schwede, T., J. Kopp, N. Guex, and M. C. Peitsch. 2003. SWISS-MODEL: an automated protein homology-modeling server. Nucleic Acids Res. 31:3381-3385.
41. Shimizu, T. S., N. Le Novere, M. D. Levin, A. J. Beavil, B. J. Sutton, and D. Bray. 2000. Molecular model of a lattice of signalling proteins involved in bacterial chemotaxis. Nat. Cell Biol. 2:792-796.
42. Shrout, A. L., D. J. Montefusco, and R. M. Weis. 2003. Template-directed assembly of receptor signaling complexes. Biochemistry 42:13379-13385.
43. Silverman, M., P. Matsumura, M. Hilmen, and M. Simon. 1977. Characterization of lambda Escherichia coli hybrids carrying chemotaxis genes. J. Bacteriol. 130:877-887.
44. Smith, R. A., and J. S. Parkinson. 1980. Overlapping genes at the cheA locus of E. coli. Proc. Natl. Acad. Sci. USA 77:5370-5374.
45. Sourjik, V., and H. C. Berg. 2004. Functional interactions between receptors in bacterial chemotaxis. Nature 428:437-441.
46. Surette, M. G., M. Levit, Y. Liu, G. Lukat, E. G. Ninfa, A. Ninfa, and J. B. Stock. 1996. Dimerization is required for the activity of the protein histidine kinase CheA that mediates signal transduction in bacterial chemotaxis. J. Biol. Chem. 271:939-945.
47. Swanson, R. V., R. B. Bourret, and M. I. Simon. 1993. Intermolecular complementation of the kinase activity of CheA. Mol. Microbiol. 8:435-441.
48. Szurmant, H., and G. W. Ordal. 2004. Diversity in chemotaxis mechanisms among the bacteria and archaea. Microbiol. Mol. Biol. Rev. 68:301-319.
49. Wadhams, G. H., and J. P. Armitage. 2004. Making sense of it all: bacterial chemotaxis. Nat. Rev. Mol. Cell Biol. 5:1024-1037.
50. Wolfe, A. J., and H. C. Berg. 1989. Migration of bacteria in semisolid agar. Proc. Natl. Acad. Sci. USA 86:6973-6977.
51. Wolfe, A. J., B. P. McNamara, and R. C. Stewart. 1994. The short form of CheA couples chemoreception to CheA phosphorylation. J. Bacteriol. 176:4483-4491.
52. Xu, W., S. C. Harrison, and M. J. Eck. 1997. Three-dimensional structure of the tyrosine kinase c-Src. Nature 385:595-602.