Matrices with compliance comparable to that of brain tissue select neuronal over glial growth in mixed cortical cultures

Biophysical Journal

Volumn 90, Issue 8, 2006, Pages 3012-3018

  Georges, Penelope C ^a,b   Miller, William J ^a,b   Meaney, David F ^a,b   Sawyer, Evelyn S ^c   Janmey, Paul A ^a,b,d,e  

^a Institute for Medicine and Engineering   (United States)

^b Department of Bioengineering ^*  (United States)

^c Sea Run Holdings Inc   (United States)

^d UNIVERSITY OF PENNSYLVANIA   (United States)

^e 1010 Vagelos Laboratories   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL RECEPTOR; F ACTIN; LAMININ; PLASTIC;

ACTIN POLYMERIZATION; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; ASTROCYTE; BRAIN DAMAGE; BRAIN TISSUE; CELL ADHESION; CELL COUNT; CELL POPULATION; CELL ULTRASTRUCTURE; COATED PARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; DISSOCIATION; EMBRYO; EMBRYO CULTURE; EXTRACELLULAR MATRIX; GEL; GLIA CELL; MITOSIS INHIBITION; NERVE CELL CULTURE; NERVE CELL GROWTH; NERVE CELL NETWORK; NERVE FIBER GROWTH; NERVE FIBER REGENERATION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERIZATION; RAT; RIGIDITY; TISSUE CULTURE; YOUNG MODULUS;

ANIMALIA;

EID: 33646204388     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1529/biophysj.105.073114     Document Type: Article

References (27)

1. Fawcett, J. W., and R. A. Asher. 1999. The glial scar and central nervous system repair. Brain Res. Bull. 49:377-391.
2. Horner, P. J., and F. H. Gage. 2000. Regenerating the damaged central nervous system. Nature. 407:963-970.
3. Pelham, R. J. Jr., and Y. Wang. 1997. Cell locomotion and focal adhesions are regulated by substrate flexibility. Proc. Natl. Acad. Sci. USA. 94:13661-13665.
4. Bischofs, I. B., and U. S. Schwarz. 2003. Cell organization in soft media due to active mechanosensing. Proc. Natl. Acad. Sci. USA. 100:9274-9279.
5. Janmey, P. A., and D. A. Weitz. 2004. Dealing with mechanics: mechanisms of force transduction in cells. Trends Biochem. Sci. 29:364-370.
6. Discher, D. E., P. Janmey, and Y. L. Wang. 2005. Tissue cells feel and respond to the stiffness of their substrate. Science. 310:1139-1143.
7. Engler, A. J., M. A. Griffin, S. Sen, C. G. Bonnemann, H. L. Sweeney, and D. E. Discher. 2004. Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments. J. Cell Biol. 166:877-887.
8. Flanagan, L. A., Y. E. Ju, B. Marg, M. Osterfield, and P. A. Janmey. 2002. Neurite branching on deformable substrates. Neuroreport. 13:2411-2415.
9. Georges, P. C., and P. A. Janmey. 2005. Cell type-specific response to growth on soft materials. J. Appl. Physiol. 98:1547-1553.
10. Balgude, A. P., X. Yu, A. Szymanski, and R. V. Bellamkonda. 2001. Agarose gel stiffness determines rate of DRG neurite extension in 3D cultures. Biomaterials. 22:1077-1084.
11. Engler, A., L. Bacakova, C. Newman, A. Hategan, M. Griffin, and D. Discher. 2004. Substrate compliance versus ligand density in cell on gel responses. Biophys. J. 86:617-628.
12. Yeung, T., P. C. Georges, L. A. Flanagan, B. Marg, M. Ortiz, M. Funaki, N. Zahir, W. Ming, V. Weaver, and P. A. Janmey. 2005. Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion. Cell Motil. Cytoskeleton. 60:24-34.
13. Woerly, S., V. D. Doan, N. Sosa, J. de Vellis, and A. Espinosa-Jeffrey. 2004. Prevention of gliotic scar formation by NeuroGel allows partial endogenous repair of transected cat spinal cord. J. Neurosci. Res. 75:262-272.
14. Lesny, P., J. De Croos, M. Pradny, J. Vacik, J. Michalek, S. Woerly, and E. Sykova. 2002. Polymer hydrogels usable for nervous tissue repair. J. Chem. Neuroanat. 23:243-247.
15. Loh, N. K., S. Woerly, S. M. Bunt, S. D. Wilton, and A. R. Harvey. 2001. The regrowth of axons within tissue defects in the CNS is promoted by implanted hydrogel matrices that contain BDNF and CNTF producing fibroblasts. Exp. Neurol. 170:72-84.
16. Teng, Y. D., E. B. Lavik, X. Qu, K. I. Park, J. Ourednik, D. Zurakowski, R. Langer, and E. Y. Snyder. 2002. Functional recovery following traumatic spinal cord injury mediated by a unique polymer scaffold seeded with neural stem cells. Proc. Natl. Acad. Sci. USA. 99:3024-3029.
17. Hirakawa, K., K. Hashizume, and T. Hayashi. 1981. Viscoelastic property of human brain - for the analysis of impact injury. No To Shinkei. 33:1057-1065.
18. Miller, K., K. Chinzei, G. Orssengo, and P. Bednarz. 2000. Mechanical properties of brain tissue in-vivo: experiment and computer simulation. J. Biomech. 33:1369-1376.
19. Michaud, S. E., L. Z. Wang, N. Korde, R. Bucki, P. K. Randhawa, J. J. Pastore, H. Falet, K. Hoffmeister, R. Kuuse, R. Uibo, J. Herod, E. Sawyer, and P. A. Janmey. 2002. Purification of salmon thrombin and its potential as an alternative to mammalian thrombins in fibrin sealants. Thromb. Res. 107:245-254.
20. Fitch, M. T., C. Doller, C. K. Combs, G. E. Landreth, and J. Silver. 1999. Cellular and molecular mechanisms of glial scarring and progressive cavitation: in vivo and in vitro analysis of inflammation-induced secondary injury after CNS trauma. J. Neurosci. 19:8182-8198.
21. Brandley, B. K., O. A. Weisz, and R. L. Schnaar. 1987. Cell attachment and long-term growth on derivatizable polyacrylamide surfaces. J. Biol. Chem. 262:6431-6437.
22. Carr, M. E. Jr., and S. L. Carr. 1995. Fibrin structure and concentration alter clot elastic modulus but do not alter platelet mediated force development. Blood Coagul. Fibrinolysis. 6:79-86.
23. Gefen, A., N. Gefen, Q. Zhu, R. Raghupathi, and S. S. Margulies. 2003. Age-dependent changes in material properties of the brain and brain-case of the rat. J. Neurotrauma. 20:1163-1177.
24. Bilston, L. E., Z. Liu, and N. Phan-Thien. 1997. Linear viscoelastic properties of bovine brain tissue in shear. Biorheology. 34:377-385.
25. Dembo, M., and Y. L. Wang. 1999. Stresses at the cell-to-substrate interface during locomotion of fibroblasts. Biophys. J. 76:2307-2316.
26. Pittier, R., F. Sauthier, J. A. Hubbell, and H. Hall. 2004. Neurite extension and in vitro myelination within three-dimensional modified fibrin matrices. J. Neurobiol. 63:1-14.
27. Bridgman, P. C., S. Dave, C. F. Asnes, A. N. Tullio, and R. S. Adelstein. 2001. Myosin IIB is required for growth cone motility. J. Neurosci. 21:6159-6169.