Identification of in vivo phosphorylation sites on human deoxycytidine kinase: Role of Ser-74 in the control of enzyme activity

Journal of Biological Chemistry

Volumn 281, Issue 8, 2006, Pages 4887-4893

  Smal, Caroline ^a   Vertommen, Didier ^a   Bertrand, Luc ^a,b,c   Ntamashimikiro, Sandrine ^a   Rider, Mark H ^a   Van Den Neste, Eric ^a,b   Bontemps, Françoise ^a  

^a DE DUVE INSTITUTE   (Belgium)

^b CLINIQUES UNIVERSITAIRES SAINT LUC   (Belgium)

^c FNRS   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYRIBONUCLEOSIDE; ENZYME ACTIVITY; HUMAN DEOXYCYTIDINE KINASE; IN VIVO PHOSPHORYLATION;

AMINO ACIDS; ANTIBODIES; CELLS; CHEMICAL REACTIONS; CHEMOTHERAPY; DIGESTIVE SYSTEM; NUCLEIC ACIDS; RADIOACTIVE ELEMENTS; TUMORS;

ENZYMES;

DEOXYCYTIDINE KINASE; PHOSPHATE; PHOSPHOPROTEIN; PHOSPHORUS 32; SERINE; SORBITOL; THREONINE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CELL STRAIN HEK293; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME DEGRADATION; ENZYME PHOSPHORYLATION; GENE OVEREXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; IN VIVO STUDY; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DEPHOSPHORYLATION; SITE DIRECTED MUTAGENESIS; TANDEM MASS SPECTROMETRY; BINDING SITE; CELL LINE; CHEMISTRY; GENE EXPRESSION REGULATION; IMMUNOBLOTTING; MASS SPECTROMETRY; METABOLISM; MUTATION; PHOSPHORYLATION; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; TUMOR CELL LINE;

MAMMALIA;

BINDING SITES; CELL LINE; CELL LINE, TUMOR; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DEOXYCYTIDINE KINASE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; IMMUNOBLOTTING; MASS SPECTROMETRY; MUTAGENESIS, SITE-DIRECTED; MUTATION; PHOSPHORYLATION; PLASMIDS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SERINE; SORBITOL; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 33646178365     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M512129200     Document Type: Article

References (38)

1. Arner, E. S., and Eriksson, S. (1995) Pharmacol. Ther. 67, 155-186
2. Plunkett, W., and Gandhi, V. (1997) in Nucleoside Analogs in Cancer Therapy (Cheson, B. D., Keating, M. J., and Plunkett, W., eds) pp. 1-35, Marcel Dekker, Inc., New York
3. Galmarini, C. M., Mackey, J. R., and Dumontet, C. (2002) Lancet Oncol. 3, 415-424
4. Pettitt, A. R. (2003) Br. J. Haematol. 121, 692-702
5. De Clercq, E. (2004) J. Clin. Virol. 30, 115-133
6. Carson, D. A., Wasson, D. B., Kaye, J., Ullman, B., Martin, D. W., Jr., Robins, R. K., and Montgomery, J. A. (1980) Proc. Natl. Acad. Sci. U. S. A. 77, 6865-6869
7. Verhoef, V., Sarup, J., and Fridland, A. (1981) Cancer Res. 41, 4478-4483
8. Orr, R. M., Talbot, D. C., Aherne, W. G., Fisher, T. C., Serafinowski, P., and Harrap, K. R. (1995) Clin. Cancer Res. 1, 391-398
9. Galmarini, C. M., Mackey, J. R., and Dumontet, C. (2001) Leukemia (Baltimore) 15, 875-890
10. Kawasaki, H., Carrera, C. J., Piro, L. D., Saven, A., Kipps, T. J., and Carson, D. A. (1993) Blood 81, 597-601
11. Stegmann, A. P., Honders, M. W., Hagemeijer, A., Hoebee, B., Willemze, R., and Landegent, J. E. (1995) Ann. Hematol. 71, 41-47
12. Arner, E. S. (1996) Leuk. Lymphoma 21, 225-231
13. Veuger, M. J., Honders, M. W., Landegent, J. E., Willemze, R., and Barge, R. M. (2000) Blood 96, 1517-1524
14. Blackstock, A. W., Lightfoot, H., Case, L. D., Tepper, J. E., Mukherji, S. K., Mitchell, B. S., Swarts, S. G., and Hess, S. M. (2001) Clin. Cancer Res. 7, 3263-3268
15. Mansson, E., Flordal, E., Liliemark, J., Spasokoukotskaja, T., Elford, H., Lagercrantz, S., Eriksson, S., and Albertioni, F. (2003) Biochem. Pharmacol. 65, 237-247
16. Goodman, G. R., Bethel, K. J., and Saven, A. (2003) Curr. Opin. Hematol. 10, 258-266
17. Robak, T. (2005) Transfus. Apher. Sci. 32, 33-44
18. Sasvari-Szekely, M., Spasokoukotskaja, T., Szoke, M., Csapo, Z., Turi, A., Szanto, I., Eriksson, S., and Staub, M. (1998) Biochem. Pharmacol. 56, 1175-1179
19. Spasokoukotskaja, T., Sasvari-Szekely, M., Keszler, G., Albertioni, F., Eriksson, S., and Staub, M. (1999) Eur. J. Cancer 35, 1862-1867
20. Cardoen, S., Van Den Neste, E., Smal, C., Rosier, J.-F., Delacauw, A., Ferrant, A., Van den Berghe, G., and Bontemps, F. (2001) Clin. Cancer Res. 7, 3559-3566
21. Csapo, Z., Sasvari-Szekely, M., Spasokoukotskaja, T., Talianidis, I., Eriksson, S., and Staub, M. (2001) Biochem. Pharmacol. 61, 191-197
22. Van Den Neste, E., Smal, C., Cardoen, S., Delacauw, A., Frankard, J., Ferrant, A., Van den Berghe, G., and Bontemps, F. (2003) Biochem. Pharmacol. 65, 573-580
23. Csapo, Z., Keszler, G., Safrany, G., Spasokoukotskaja, T., Talianidis, I., Staub, M., and Sasvari-Szekely, M. (2003) Biochem. Pharmacol. 65, 2031-2039
24. Smal, C., Cardoen, S., Bertrand, L., Delacauw, A., Ferrant, A., Van den Berghe, G., Van Den Neste, E., and Bontemps, F. (2004) Biochem. Pharmacol. 68, 95-103
25. Wessel, D., and Flugge, U. I. (1984) Anal. Biochem. 138, 141-143
26. Bradford, M. M. (1976) Anal. Biochem. 72, 248-254
27. Hatzis, P., Al-Madhoon, A. S., Jullig, M., Petrakis, T. G., Eriksson, S., and Talianidis, I. (1998) J. Biol. Chem. 273, 30239-30243
28. Woods, A., Vertommen, D., Neumann, D., Turk, R., Bayliss, J., Schlattner, U., Wallimann, T., Carling, D., and Rider, M. H. (2003) J. Biol. Chem. 278, 28434-28442
29. Chottiner, E. G., Shewach, D. S., Datta, N. S., Ashcraft, E., Gribbin, D., Ginsburg, D., Fox, I. H., and Mitchell, B. S. (1991) Proc. Natl. Acad. Sci. U. S. A. 88, 1531-1535
30. Bohman, C., and Eriksson, S. (1988) Biochemistry 27, 4258-4265
31. Johansson, M., and Karlsson, A. (1995) Biochem. Pharmacol. 50, 163-168
32. Blom, N., Gammeltoft, S., and Brunak, S. (1999) J. Mol. Biol. 294, 1351-1362
33. Sabini, E., Ort, S., Monnerjahn, C., Konrad, M., and Lavie, A. (2003) Nat. Struct. Biol. 10, 513-519
34. Martensen, T. M. (1984) Methods Enzymol. 107, 3-23
35. Koenig, M., and Grabe, N. (2004) Bioinformatics 20, 3620-3627
36. Wang, L. M., and Kucera, G. L. (1994) Biochim. Biophys. Acta 1224, 161-167
37. Spasokoukotskaja, T., Csapo, Z., Sasvari-Szekely, M., Virga, S., Talianidis, I., Eriksson, S., and Staub, M. (2000) Adv. Exp. Med. Biol. 486, 281-285
38. Keszler, G., Spasokoukotskaja, T., Csapo, Z., Talianidis, I., Eriksson, S., Staub, M., and Sasvari-Szekely, M. (2004) Biochem. Pharmacol. 67, 947-955