High expression of human carboxypeptidase M in Pichia pastoris. Purification and partial characterization

Brazilian Journal of Medical and Biological Research

Volumn 39, Issue 2, 2006, Pages 211-217

  Craveiro, R B ^a   Ramalho, J D ^b   Chagas, J R ^b   Wang, P H M ^a   Casarini, D ^a   Pesquero, J L ^b,c   Araujo R C ^b   Pesquero, J B ^a  

^a FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

^b UNIVERSIDADE DE MOGI DAS CRUZES   (Brazil)

^c FEDERAL UNIVERSITY OF MINAS GERAIS   (Brazil)

Author keywords

Human carboxypeptidase M; Kinins; Pichia pastoris; Recombinant protein

Indexed keywords

CARBOXYPEPTIDASE; CARBOXYPEPTIDASE M; COMPLEMENTARY DNA; RNA; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; FERMENTATION MEDIUM; GEL FILTRATION CHROMATOGRAPHY; GENE EXPRESSION; GENETIC TRANSFECTION; HUMAN; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; PATHOPHYSIOLOGY; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN FUNCTION; PROTEIN GLYCOSYLATION; PROTEIN PURIFICATION; PROTEIN SECRETION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SEPARATION TECHNIQUE;

PICHIA PASTORIS;

EID: 33646173465     PISSN: 0100879X     EISSN: 1414431X     Source Type: Journal    
DOI: 10.1590/S0100-879X2006000200007     Document Type: Article

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