Effect of mutation of two critical glutamic acid residues on the activity and stability of human carboxypeptidase M and characterization of its signal for glycosylphosphatidylinositol anchoring

Biochemical Journal

Volumn 370, Issue 2, 2003, Pages 567-578

  Tan, Fulong ^a   Balsitis, Scott ^a   Black, Judy K ^a   Blöchl, Andrea ^a   Mao, Ji Fang ^a   Becker, Robert P ^a   Schacht, David ^a   Skidgel, Randal A ^a  

^a UNIVERSITY OF ILLINOIS   (United States)

Author keywords

Membrane anchoring; Peptidase; Peptide metabolism; Recombinant protein expression; Site directed mutagenesis

Indexed keywords

CARBOXYLIC ACIDS; CATALYST ACTIVITY; CONFORMATIONS; ENZYMES; MUTAGENESIS;

MOLECULAR MASSES;

BIOCHEMISTRY;

4 AMINOBENZOYLARGININE; ALANINE; ARGININE DERIVATIVE; CARBOXYPEPTIDASE; CARBOXYPEPTIDASE M; GLUTAMIC ACID; GLUTAMINE; GLYCOSYLPHOSPHATIDYLINOSITOL; PROLINE; SEPHAROSE; SERINE; THREONINE; TRYPSIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BACULOVIRUS; CELL MEMBRANE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME STABILITY; GLYCOSYLATION; HUMAN; HUMAN CELL; INSECT CELL; MOLECULAR WEIGHT; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY; VIRUS INFECTION;

BACULOVIRIDAE; GENETIC VECTORS; GLUTAMIC ACID; GLYCOSYLPHOSPHATIDYLINOSITOLS; HEAT; HUMANS; MEMBRANE PROTEINS; METALLOENDOPEPTIDASES; MUTATION; PROTEIN DENATURATION; PROTEIN SORTING SIGNALS; RECOMBINANT PROTEINS;

ANIMALIA; INSECTA; UNIDENTIFIED BACULOVIRUS;

EID: 0037335653     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20021495     Document Type: Article

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