p16INK4a promoter methylation and 9p21 allelic loss in colorectal carcinomas: relation with immunohistochemical p16INK4a expression and with tumor budding

Human Pathology

Volumn 37, Issue 5, 2006, Pages 578-585

  Prall, Friedrich ^a   Ostwald, Christiane ^a   Weirich, Volker ^a   Nizze, Horst ^a  

^a UNIVERSITY OF ROSTOCK   (Germany)

Author keywords

Colorectal carcinoma; Laser capture microdissection; Methylation specific PCR; p16INK4a promoter methylation; Tumor budding

Indexed keywords

CYTOKERATIN; PROTEIN P16INK4A;

ADULT; AGED; ALLELE; ARTICLE; CARCINOMA CELL; CELL DIVISION; COLORECTAL CARCINOMA; CONTROLLED STUDY; CORRELATION ANALYSIS; DNA DETERMINATION; FEMALE; GENE MUTATION; HETEROZYGOSITY; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; LASER CAPTURE MICRODISSECTION; LINEAR REGRESSION ANALYSIS; MAJOR CLINICAL STUDY; MALE; METHYLATION; MOSAICISM; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; TISSUE HOMOGENATE; UPREGULATION;

ADENOCARCINOMA; ADULT; AGED; AGED, 80 AND OVER; CELL MOVEMENT; CHROMOSOMES, HUMAN, PAIR 9; COLORECTAL NEOPLASMS; CYCLIN-DEPENDENT KINASE INHIBITOR P16; DNA METHYLATION; DNA, NEOPLASM; FEMALE; HUMANS; IMMUNOHISTOCHEMISTRY; LASERS; LOSS OF HETEROZYGOSITY; MALE; MICRODISSECTION; MIDDLE AGED; TUMOR MARKERS, BIOLOGICAL;

EID: 33646158891     PISSN: 00468177     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.humpath.2006.01.005     Document Type: Article

References (20)

1. Esteller M., and Herman J.G. Cancer as an epigenetic disease: DNA methylation and chromatin alterations in human tumors. J Pathol 196 (2002) 1-7
2. Eads C.A., Danenberg K.D., Kawakami K., et al. CpG island hypermethylation in human colorectal carcinomas is not associated with DNA methyltransferase overexpression. Cancer Res 59 (1999) 2302-2306
3. Jones P.A., and Laird P.W. Cancer epigenetics comes of age. Nat Genet 21 (1999) 163-167
4. Burri N., Shwa P., Bouzourene H., et al. Methylation silencing and mutations of the p14ARF and p16INK4a genes in colon cancer. Lab Invest 81 (2001) 217-229
5. Rocco J.W., and Sidransky D. p16 (MTS-1/CKDN2/INK4a) in cancer progression. Exp Cell Res 264 (2001) 42-55
6. Vo Q.N., Geradts J., Boudreau D.A., et al. CDKN2a promoter methylation in gastric adenocarcinomas: clinical variables. Hum Path 33 (2002) 1200-1204
7. Lerma E., Esteller M., Herman J.G., et al. Alterations of the p16/Rb/cyclin D1 pathway in vulvar carcinoma, vulvar intraepithelial neoplasia, and lichen sclerosus. Hum Path 33 (2002) 1120-1125
8. Palmqvist R., Rutegard J.N., Bozoky B., et al. Human colorectal cancer with an intact p16/cyclinD1/pRB pathway have up-regulated p16 expression and decreased proliferation in small invasive tumor clusters. Am J Pathol 157 (2000) 1947-1953
9. INK4a and is a region of low proliferation. Am J Pathol 159 (2001) 1613-1617
10. Gabbert H., Wagner R., Moll R., et al. Tumour dedifferentiation: an important step in tumour invasion. Clin Expl Met 3 (1985) 257-279
11. Ueno H., Murphy J., Jass J.R., et al. Tumour "budding" as an index to estimate the potential of aggressiveness in rectal cancer. Histopathology 40 (2002) 127-132
12. Prall F., Nizze H., and Barten M. Tumour budding as prognostic factor in stage I/II colorectal carcinoma. Histopathology 47 (2005) 17-24
13. INK4a and an intact Rb pathway illustrated by a model system of basal cell carcinoma. Cancer Res 63 (2003) 1737-1742
14. + tumor-infiltrating lymphocytes in stage III colorectal carcinomas with and without microsatellite instability. Hum Path 35 (2004) 808-818
15. Herman J.G., Graff J.R., Myohanen S., et al. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci U S A 93 (1996) 9821-9826
16. Raizis A.M., Schmitt F., and Jost J. A bisulfite method of 5-methylcytosine mapping that minimizes template degradation. Anal Biochem 226 (1995) 161-166
17. 6-methylguanine-DNA-methyltransferase is associated with the presence of G:C to A:T transition mutations in p53 in human colorectal tumorigenesis. Cancer Res 61 (2001) 4689-4692
18. Chan A.O., Issa J.P., Morris J.S., et al. Concordant CpG island methylation in hyperplastic polyposis. Am J Pathol 160 (2002) 529-536
19. Baak J.P.A. Manual of quantitative pathology in cancer diagnosis and prognosis (1991), Springer Verlag, Berlin
20. INK4a protein expression by promoter hypermethylation between left- and right-sided primary colorectal carcinomas. Int J Oncol 23 (2003) 1009-1013