Application of public-domain statistical analysis software for evaluation and comparison of comet assay data

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 604, Issue 1-2, 2006, Pages 71-82

  Verde, Pablo E ^a   Geracitano, Laura A ^b   Amado, Lílian L ^b,c   Rosa, Carlos E ^b,c   Bianchini, Adalto ^b,c   Monserrat, José M ^b,c  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b FEDERAL UNIVERSITY OF RIO GRANDE   (Brazil)

^c FURG   (Brazil)

Author keywords

Akaike information criteria; Comet assay; Estuarine organisms; Model selection; Statistical modeling; Tail moment

Indexed keywords

ANALYTIC METHOD; ARTICLE; BLOOD SAMPLING; CELL SUSPENSION; COMET ASSAY; COMPUTER ANALYSIS; COMPUTER PROGRAM; CONTROLLED STUDY; ESTUARY; EVALUATION; FLOUNDER; LOGISTIC REGRESSION ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROBABILITY; RISK ASSESSMENT; STATISTICAL ANALYSIS; STATISTICAL MODEL; TELEOST; TOXICITY TESTING; VARIANCE;

ANIMALS; COMET ASSAY; DNA DAMAGE; FLOUNDER; MUTAGENICITY TESTS; PROBABILITY; SOFTWARE;

LAEONEREIS ACUTA; NEREIDIDAE; PARALICHTHYS; PLEURONECTOIDEI; POLYCHAETA; TELEOSTEI;

EID: 33645994835     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2006.01.002     Document Type: Article

References (26)

1. Fairbain D.W., Olive P.L., and O'Neill K.L. The comet assay: a comprehensive review. Mutat. Res. 339 (1995) 37-59
2. Belpaeme K., Cooreman K., and Kirsh-Volders M. Development and validation of the in vivo alkaline comet assay for detecting genomic damage in marine flatfish. Mutat. Res. 145 (1998) 167-184
3. Mitchelmore C.L., and Chipman J.K. DNA strand breakage in aquatic organisms and the potential value of the comet assay in environmental monitoring. Mutat. Res. 399 (1998) 135-147
4. Helma C., and Uhl M. A public domain image-analysis program for the single-cell-gel-electrophoresis (comet) assay. Mutat. Res. 466 (2000) 9-15
5. Duez P., Dehon G., Kumps A., and Dubois J. Statistics of the comet assay: a key to discriminate between genotoxic effects. Mutagenesis 18 (2003) 159-166
6. Wiklund S.J., and Agurell E. Aspects of design and statistical analysis in the comet assay. Mutagenesis 18 (2003) 167-175
7. Lovell D.P., Thomas G., and Dubow R. Issues related to the experimental design and subsequent statistical analysis of in vivo and in vitro comet studies. Teratogenesis Carcinog. Mutagen. 19 (1999) 109-119
8. Mood A.M., Graybill F.A., and Boes D.C. Introduction to the Theory of Statistics. 3rd ed. (1974), McGraw-Hill Series in Probability and Statistics, New York
9. Montgomery D.C. Design and Analysis of Experiments. 2nd ed. (1984), John Wiley & Sons, New York
10. Ejchart A., and Sadlej-Sosnowska N. Statistical evaluation and comparison of comet assay results. Mutat. Res. 534 (2003) 85-92
11. Debon G., Bogaerts P., Duez P., Catoire L., and Dubois J. Curve fitting of combined comet intensity profiles: a new global concept to quantify DNA damage by the comet assay. Chemomet. Intell. Lab. Syst. 73 (2004) 235-243
12. Cox D.R., and Oakes D. Analysis of Survival Data (1984), Chapman & Hall, London
13. R Development Core Team, R: a language and environment for statistical computing, R Foundation for Statistical Computing, Vienna, Austria, 2004. ISBN 3-900051-07-0, URL http://www.r-project.org/.
14. Therneau T.M., Grambsch P.M., and Pankratz V.S. Penalized survival models and frailty. J. Computat. Graph. Stat. 12 (2003) 156-175
15. W.N. Venalbles, B.D. Ripley, 4th ed., Springer-Verlag Inc., New York, 2003.
16. Baumgarten M.G.Z., and Niencheski L.F.H. O estuário da laguna dos Patos: variações de alguns parâmetros físico-químicos da água e metais associados ao material em suspensão. Ciência e Cultura 42 (1990) 390-396
17. Baumgarten M.G.Z., Aznar C.B., Rocha J.M., Almeida M.T., and Kinas P.G. Contaminação química das águas receptoras do principal efluente doméstico da cidade do Rio Grande (RS). Atlântica 20 (1998) 35-94
18. M.G.Z. Baumgarten, L.F.H. Niencheski, Avaliação da qualidade hidroquímica da área portuária da cidade do Rio Grande (RS), Documentos Técnicos - Oceanografia, Editora da FURG, Rio Grande, 1998.
19. S. Marcuzzo, S.M. Pagel, M.I.S. Chiapetii, A Reserva da Biosfera da Mata Atlântica no Rio Grande do Sul - Situação atual, ações e perspectives, In: J.P.O. Costa, C.F. Lino, J.L. Albuquerque (Eds.), Série Cadernos da Biosfera da Mata Atlântica, Conselho Nacional da Reserva da Biosfera Mata Atlântica, São Paulo, 1998, 60 pp.
20. Baisch P.R., Niencheski L.F., and Lacerda L.D. Trace metal distribution in sediments of the Patos Lagoon Estuary, Brazil. In: Seeliger U., Lacerda L.D., and Patchineelam S.R. (Eds). Metals in Coastal Environments of Latin America (1988), Springer Verlag, New York, Berlin, Heidelberg 59-64
21. P.M. Medeiros, M.C. Bícego, R.M. Castelao, C. Del Rosso, G. Fillmann, A.J. Zamboni, Natural and anthropogenic hydrocarbon inputs to sediments of Patos Lagoon Estuary, Brazil. Environ. Int., in press.
22. Singh N.P., McCoy M.T., Tice R.R., and Schneider E.L. A simple technique for quantification of low levels of DNA damage in individual cells. Exp. Cell Res. 175 (1988) 184-191
23. Tice R.R., Agureli E., Anderson D., Burlinson B., Hartmann A., Kobayashi H., Miyamae Y., Rojas E., Ryu J.C., and Sasaki Y.F. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen. 35 (2000) 206-221
24. McCullagh P., and Nelder J.A. Generalized Linear ModelsEDN 2nd ed. (1989), Chapman & Hall, London
25. Burnham K.P., and Anderson D.A. Model Selection and Multi-Model Inference: A Practical Information-Theoretic Approach. 2nd ed. (2002), Springer-Verlag, New York
26. T. Therneau, T. Lumley, Survival: survival analysis, including penalized likelihood, R.package version 2.17, 2004.