Exploring the cupin-type metal-coordinating signature of acetylacetone dioxygenase Dke1 with site-directed mutagenesis: Catalytic reaction profile and Fe2+ binding stability of Glu-69 → Gln mutant

Journal of Molecular Catalysis B: Enzymatic

Volumn 39, Issue 1-4, 2006, Pages 171-178

  Straganz, Grit D ^a   Egger, Sigrid ^a   Aquino, Gianluca ^b   D'Auria, Sabato ^b   Nidetzky, Bernd ^a  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^b INSTITUTE OF PROTEIN BIOCHEMISTRY   (Italy)

Author keywords

Diketone; Cupin; C single bond C bond cleavage; Metalloenzyme; Non heme iron

Indexed keywords

AMINO ACIDS; CATALYSIS; ENZYMES; FREE ENERGY; KETONES; MUTAGENESIS; PERTURBATION TECHNIQUES; PROTEINS; REDUCTION;

ACINETOBACTER JOHNSONII; CUPIN; DIKETONE; METALLOENZYME; MUTANT; NON-HEME IRON;

IRON ALLOYS;

1,3 DIKETONE; ACETYLACETONE; AMINO ACID; BACTERIAL ENZYME; BACTERIAL PROTEIN; CARBON; CUPIN; DIOXYGENASE; HEME DERIVATIVE; IRON; METAL; METALLOPROTEIN; OXYGEN; UNCLASSIFIED DRUG;

ACINETOBACTER; AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CHEMICAL ANALYSIS; CHEMICAL BOND; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME STRUCTURE; IRON BINDING CAPACITY; NONHUMAN; PROTEIN FAMILY; REDUCTION; SITE DIRECTED MUTAGENESIS; WILD TYPE;

ACINETOBACTER; ACINETOBACTER JOHNSONII; BACTERIA (MICROORGANISMS);

EID: 33645980325     PISSN: 13811177     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molcatb.2006.01.019     Document Type: Article

References (22)

1. Costas M., Mehn M.P., Jensen M.P., and Que Jr. L. Chem. Rev. 104 (2004) 939-986
2. Hegg E.L., and Que Jr. L. Eur. J. Biochem. 250 (1997) 625-629
3. Dunwell J.M., Purvis A., and Khuri S. Phytochemistry 65 (2004) 7-17
4. Dunwell J.M., Culham A., Carter C.E., Sosa-Aguirre C.R., and Goodenough P.W. Trends Biochem. Sci. 26 (2001) 740-746
5. Gane P.J., Dunwell J.M., and Warwicker J. J. Mol. Evol. 46 (1998) 488-493
6. Woo E., Dunwell J., Goodenough P.W., Marvier A.C., and Pickersgill R.W. Nat. Struct. Biol. 7 (2000) 1036-1040
7. Tanner A., and Bornemann S. J. Bacteriol. 182 (2002) 5271-5273
8. Fu W., and Oriel P. Extremophiles 2 (1998) 439-446
9. Kwon Y.H., and Stipanuk M.H. Am. J. Physiol. Endocrinol. Metab. 280 (2001) E804-E815
10. Pochapsky T.C., Pochapsky S.S., Ju T., Mo H., Al-Mjeni F., and Maroney M.J. Nat. Struct. Biol. 9 (2002) 966-972
11. Fusetti F., Schröter K.H., Steiner R.A., van Noort P.I., Pijning T., Rozeboom H.J., Kalk K.H., Egmond M.R., and Dijkstra B.W. Structure 10 (2002) 259-268
12. Straganz G., Brecker L., Weber H.J., Steiner W., and Ribbons D.W. Biochem. Biophys. Res. Commun. 297 (2002) 232-236
13. Straganz G.D., Glieder A., Brecker L., Ribbons D.W., and Steiner W. Biochem. J. 369 (2003) 573-581
14. Steiner R.A., Kalk K.H., and Dijkstra B.W. Proc. Natl. Acad. Sci. U.S.A. 99 (2002) 16625-16630
15. Straganz G., Hofer H., Steiner W., and Nidetzky B. J. Am. Chem. Soc. 126 (2004) 12202-12203
16. Straganz G., and Nidetzky B. J. Am. Chem. Soc. 127 (2005) 12306-12314
17. G.R. Stranzl, X-ray crystal structure of acetylacetone-cleaving dioxygenase of A. johnsonii and NMR evidence for a strong short hydrogen bond in the active site of HbHNL. Ph.D. thesis, Karl Franzens University of Graz, Graz, Austria, 2002.
18. Wang W., and Malcolm B.A. Biotechniques 26 (1999) 680-682
19. Straganz G., Slavica A., Hofer H., Mandl U., Steiner W., and Nidetzky B. Biocatal. Biotransform. 23 (2005) 261-269
20. Skerra A., and Schmidt T.G. Methods Enzymol. 326 (2000) 271-304
21. Higgins T. Clin. Chem. 27 (1981) 1619-1620
22. Johnson-Winters K., Purpero V.M., Kavana M., Nelson T., and Moran G.R. Biochemistry 42 (2003) 2072-2080