Evaluation of intranuclear BrdU detection procedures for use in multicolor flow cytometry

Cytometry Part A

Volumn 69, Issue 4, 2006, Pages 249-259

  Rothaeusler, Kristina ^a   Baumgarth, Nicole ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

B 1 a cells; Intracellular staining; Lymphocyte dynamics; Splenic B cell subsets

Indexed keywords

BROXURIDINE; FLUORESCENT DYE; PARAFORMALDEHYDE; SAPONIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; B LYMPHOCYTE; CELL MATURATION; CELL POPULATION; CELL PROLIFERATION; CELL SURFACE; CONTROLLED STUDY; EVALUATION; FEMALE; FLOW CYTOMETRY; LYMPHOCYTE SUBPOPULATION; MEASUREMENT; MOUSE; NONHUMAN; PRIORITY JOURNAL; QUALITY CONTROL; SPLEEN CELL; STAINING;

ANIMALS; B-LYMPHOCYTE SUBSETS; BROMODEOXYURIDINE; CELL NUCLEUS; CELL PROLIFERATION; FEMALE; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; FORMALDEHYDE; INDICATORS AND REAGENTS; MICE; MICE, INBRED BALB C; POLYMERS; SAPONINS; SPLEEN; STAINING AND LABELING; THYMUS GLAND;

EID: 33645956193     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20252     Document Type: Article

References (36)

1. Perfetto SP, Chattopadhyay PK, Roederer M. Seventeen-colour flow cytometry: Unravelling the immune system. Nat Rev Immunol 2004;4:648-655.
2. Baumgarth N, Roederer M. A practical approach to multicolor flow cytometry for immunophenotyping. J Immunol Methods 2000;243(1/2):77-97.
3. Baumgarth N. B-cell immunophenotyping. Methods Cell Biol 2004;75:643-662.
4. Loder F, Mutschler B, Ray RJ, Paige CJ, Sideras P, Torres R, Lamers MC, Carsetti R. B cell development in the spleen takes place in discrete steps and is determined by the quality of B cell receptor-derived signals. J Exp Med 1999;190:75-89.
5. Kantor AB. Herzenberg LA. Origin of murine B cell lineages. Annu Rev Immunol 1993;11:501-538.
6. Berland R, Wortis HH. Origins and functions of B-1 cells with notes on the role of CD5. Annu Rev Immunol 2002;20:253-300.
7. Won WJ, Kearney JF. CD9 is a unique marker for marginal zone B cells, B1 cells, and plasma cells in mice. J Immunol 2002;168:5605-5611.
8. Wells SM, Kantor AB, Stall AM. CD43 (S7) expression identifies peripheral B cell subsets. J Immunol 1994;153:5503-5515.
9. Ochsenbein AF, Fehr T, Lutz C, Suter M, Brombacher F, Hengartner H, Zinkernagel RM. Control of early viral and bacterial distribution and disease by natural antibodies. Science 1999;286(5447):2156-2159.
10. Baumgarth N, Herman OC, Jager GC, Brown LE, Herzenberg LA, Chen J. B-1 and B-2 cell-derived immunoglobulin M antibodies are nonredundant components of the protective response to influenza virus infection. J Exp Med 2000;192:271-280.
11. Herzenberg LA. B-1 cells: The lineage question revisited. Immunol Rev 2000;175:9-22.
12. Deenen GJ, Kroese FG. Murine peritoneal Ly-1 B cells do not turn over rapidly. Ann N Y Acad Sci 1992;651:70-71.
13. Forster I, Vieira P, Rajewsky K. Flow cytometric analysis of cell proliferation dynamics in the B cell compartment of the mouse. Int Immunol 1989;1:321-331.
14. Forster I, Rajewsky K. The bulk of the peripheral B-cell pool in mice is stable and not rapidly renewed from the bone marrow. Proc Natl Acad Sci USA 1990;87:4781-4784.
15. Schittek B, Rajewsky K. Maintenance of B-cell memory by long-lived cells generated from proliferating precursors. Nature 1990;346:749-751.
16. Schittek B, Rajewsky K, Forster I. Dividing cells in bone marrow and spleen incorporate bromodeoxyuridine with high efficiency. Eur J Immunol 1991;21(1):235-238.
17. Carayon P, Bord A. Identification of DNA-replicating lymphocyte subsets using a new method to label the bromo-deoxyuridine incorporated into the DNA. J Immunol Methods 1992;147:225-230.
18. Penit C, Vasseur F. Phenotype analysis of cycling and postcycling thymocytes: Evaluation of detection methods for BrdUrd and surface proteins. Cytometry 1993;14:757-763.
19. Leif RC, Stein JH, Zucker RM. A short history of the initial application of anti-5-BrdU to the detection and measurement of S phase. Cytometry A 2004;58(1):45-52.
20. Sander B, Andersson J, Andersson IJ. Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure. Immunol Rev 1991;119:65-93.
21. Willingham MC, Pastan I. Ultrastructural immunocytochemical localization of the transferrin receptor using a monoclonal antibody in human KB cells. J Histochem Cytochem 1985;33:59-64.
22. BrDU Flow Kit Manual, BD Biosciences Pharmingen, 2004.
23. Tough DF, Sprent J. Measurement of T and B cell turnover with BrdU. In: Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W, editors. Current Protocols in Immunology. Vol. 1. New York: Wiley; 1996. p 4.7.1-4.7.6.
24. Maecker HT, Frey T, Nomura LK, Trotter J. Selecting fluorochrome conjugates for maximum sensitivity. Cytometry A 2004;62:169-173.
25. Osmond DG. Population dynamic: of bone marrow B lymphocytes. Immunol Rev 1986;93:103-124.
26. Roederer M. Spectral compensation for flow cytometry: Visualization artifacts, limitations, and caveats. Cytometry 2001;45:194-205.
27. Leduc EH, Scott GB, Avrameas S. Ultrastructural localization of intracellular immune globulins in plasma cells and lymphoblasts by enzyme-labeled antibodies. J Histochem Cytochem 1969;17:211-224.
28. McLean IW, Nakane PK. Periodate-lysine-paraformaldehyde fixative. A new fixation for immunoelectron microscopy. J Histochem Cytochem 1974;22:1077-1083.
29. Andersson U, Hallden G, Persson U, Hed J, Moller G, DeLey M. Enumeration of IFN-γ-producing cells by flow cytometry. Comparison with fluorescence microscopy. J Immunol Methods 1988;112(1):139-142.
30. Hayakawa K, Hardy RR, Herzenberg LA. Peritoneal Ly-1 B cells: Genetic control, autoantibody production. Increased λ light chain expression. Eur J Immunol 1986;16:450-456.
31. Baumgarth N, Bigos M. Optimization of emission optics for multicolor flow cytometry. Methods Cell Biol 2004;75:3-22.
32. Klinman DM, Holmes KL. Differences in the repertoire expressed by peritoneal and splenic Ly-1 (CD5)+ B cells. J Immunol 1990;144:4520-4525.
33. Tumang JR, Hastings WD, Bai C, Rothstein TL. Peritoneal and splenic B-1 cells are separable by phenotypic, functional, and transcriptomic characteristics. Eur J Immunol 2004;34:2158-2167.
34. Kretschmer K, Jungehloud A, Stopkowicz J, Stoermann B, Hoffmann R, Weiss S. Antibody repertoire and gene expression profile: Implications for different developmental and functional traits of splenic and peritoneal B-1 lymphocytes. J Immunol 2003;171:1192-1201.
35. Wardemann H, Boehm T, Dear N, Carsetti R. B-1a B cells that link the innate and adaptive immune responses are lacking in the absence of the spleen. J Exp Med 2002;195:771-780.
36. Kretschmer K, Stopkowicz J, Scheffer S, Greten TF, Weiss S. Maintenance of peritoneal B-1a lymphocytes in the absence of the spleen. J Immunol 2004;173(1):197-204.