Lentiviral short hairpin ribonucleic acid-mediated knockdown of GLUT4 in 3T3-L1 adipocytes

Endocrinology

Volumn 147, Issue 5, 2006, Pages 2245-2252

  Liao, Wei ^a   Audrey Nguyen, M T ^a   Imamura, Takeshi ^a   Singer, Oded ^b   Verma, Inder M ^b   Olefsky, Jerrold M ^a  

^a UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

^b SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINOPEPTIDASE; GLUCOSE TRANSPORTER; GLUCOSE TRANSPORTER 1; GLUCOSE TRANSPORTER 4; HYBRID PROTEIN; INSULIN; SHORT HAIRPIN RNA; SMALL INTERFERING RNA; VIRUS RNA;

ADIPOCYTE; ADIPOGENESIS; ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL LINE; CONTROLLED STUDY; CYTOLOGY; EMBRYO; FIBROBLAST; GENE CONSTRUCT; GENE SEQUENCE; GENE TARGETING; GENETIC TRANSFECTION; GLUCOSE TRANSPORT; HUMAN; HUMAN CELL; LENTIVIRINAE; MOLECULAR DYNAMICS; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEFICIENCY; PROTEIN EXPRESSION; PROTEIN TARGETING; SIGNAL TRANSDUCTION; TRANSGENE; VALIDATION PROCESS;

3T3-L1 CELLS; ADIPOCYTES; ANIMALS; AZO COMPOUNDS; BIOLOGICAL TRANSPORT; CELL DIFFERENTIATION; CELL LINE; CLONING, MOLECULAR; ELECTROPORATION; FLUORESCENT DYES; GENE SILENCING; GLUCOSE; GLUCOSE TRANSPORTER TYPE 4; GREEN FLUORESCENT PROTEINS; HUMANS; IMMUNOBLOTTING; INSULIN; LENTIVIRUS; LUMINESCENT PROTEINS; MICE; MODELS, BIOLOGICAL; PLASMIDS; RNA, SMALL INTERFERING; SIGNAL TRANSDUCTION; TIME FACTORS; TRANSFECTION;

EID: 33645885646     PISSN: 00137227     EISSN: 00137227     Source Type: Journal    
DOI: 10.1210/en.2005-1638     Document Type: Article

References (28)

1. Myers Jr MG, White MF 1996 Insulin signal transduction and the IRS proteins. Annu Rev Pharmacol Toxicol 36:615-658
2. Watson RT, Kanzaki M, Pessin JE 2004 Regulated membrane trafficking of the insulin-responsive glucose transporter 4 in adipocytes. Endocr Rev 25:177-204
3. Ross SA, Gulve EA, Wang M 2004 Chemistry and biochemistry of type 2 diabetes. Chem Rev 104:1255-1282
4. Green H, Meuth M 1974 An established pre-adipose cell line and its differentiation in culture. Cell 3:127-133
5. Green H, Kehinde O 1975 An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion. Cell 5:19-27
6. Green H, Kehinde O 1976 Spontaneous heritable changes leading to increased adipose conversion in 3T3 cells. Cell 7:105-113
7. Kurreck J 2003 Antisense technologies. Improvement through novel chemical modifications. Eur J Biochem 270:1628-1644
8. Imamura T, Huang J, Dalle S, Ugi S, Usui I, Luttrell LM, Miller WE, Lefkowitz RJ, Olefsky JM 2001 β-Arrestin-mediated recruitment of the Src family kinase Yes mediates endothelin-1-stimulated glucose transport. J Biol Chem 276:43663-43667
9. Ishibashi KI, Imamura T, Sharma PM, Huang J, Ugi S, Olefsky JM 2001 Chronic endothelin-1 treatment leads to heterologous desensitization of insulin signaling in 3T3-L1 adipocytes. J Clin Invest 107:1193-1202
10. Rubinson DA, Dillon CP, Kwiatkowski AV, Sievers C, Yang L, Kopinja J, Rooney DL, Ihrig MM, McManus MT, Gertler FB, Scott ML, Van Parijs L 2003 A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Nat Genet 33:401-406
11. Tiscornia G, Singer O, Ikawa M, Verma IM 2003 A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA. Proc Natl Acad Sci USA 100:1844-1848
12. Naldini L, Blomer U, Gallay P, Ory D, Mulligan R, Gage FH, Verma IM, Trono D 1996 In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 272:263-267
13. Dull T, Zufferey R, Kelly M, Mandel RJ, Nguyen M, Trono D, Naldini L 1998 A third-generation lentivirus vector with a conditional packaging system. J Virol 72:8463-6471
14. Pfeifer A, Kessler T, Silletti S, Cheresh DA, Verma IM 2000 Suppression of angiogenesis by lentiviral delivery of PEX, a noncatalytic fragment of matrix metalloproteinase 2. Proc Natl Acad Sci USA 97:12227-12232
15. Naldini L, Blomer U, Gage FH, Trono D, Verma IM 1996 Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector. Proc Natl Acad Sci USA 93:11382-11388
16. Imamura T, Vollenweider P, Egawa K, Clodi M, Ishibashi K, Nakashima N, Ugi S, Adams JW, Brown JH, Olefsky JM 1999 Gα-q/11 protein plays a key role in insulin-induced glucose transport in 3T3-L1 adipocytes. Mol Cell Biol 19:6765-6774
17. Hansen JB, Petersen RK, Larsen BM, Bartkova J, Alsner J, Kristiansen K 1999 Activation of peroxisome proliferator-activated receptor gamma bypasses the function of the retinoblastoma protein in adipocyte differentiation. J Biol Chem 274:2386-2393
18. Ding ST, McNeel RL, Mersmann HJ 2002 Modulation of adipocyte determination and differentiation-dependent factor 1 by selected polyunsaturated fatty acids. In Vitro Cell Dev Biol Anim 38:352-357
19. Jiang H, Li J, Katz EB, Charron MJ 2001 GLUT4 ablation in mice results in redistribution of IRAP to the plasma membrane. Biochem Biophys Res Commun 284:519-525
20. Abel ED, Graveleau C, Betuing S, Pham M, Reay PA, Kandror V, Kupriyanova T, Xu Z, Kandror KV 2004 Regulation of insulin-responsive aminopeptidase expression and targeting in the insulin-responsive vesicle compartment of glucose transporter isoform 4-deficient cardiomyocytes. Mol Endocrinol 18:2491-2501
21. Carvalho E, Schellhorn SE, Zabolotny JM, Martin S, Tozzo E, Peroni OD, Houseknecht KL, Mundt A, James DE, Kahn BB 2004 GLUT4 overexpression or deficiency in adipocytes of transgenic mice alters the composition of GLUT4 vesicles and the subcellular localization of GLUT4 and insulin-responsive aminopeptidase. J Biol Chem 279:21598-21605
22. Elbashir SM, Harborth J, Weber K, Tuschl T 2002 Analysis of gene function in somatic mammalian cells using small interfering RNAs. Methods 26:199-213
23. Hannon GJ, Rossi JJ 2004 Unlocking the potential of the human genome with RNA interference. Nature 431:371-378
24. Usui I, Imamura T, Huang J, Satoh H, Olefsky JM 2003 Cdc42 is a Rho GTPase family member that can mediate insulin signaling to glucose transport in 3T3-L1 adipocytes. J Biol Chem 278:13765-13774
25. Jiang ZY, Zhou QL, Coleman KA, Chouinard M, Boese Q, Czech MP 2003 Insulin signaling through Akt/protein kinase B analyzed by small interfering RNA-mediated gene silencing. Proc Natl Acad Sci USA 100:7569-7574
26. Mitra P, Zheng X, Czech MP 2004 RNAi-based analysis of CAP, Cbl, and CrkII function in the regulation of GLUT4 by insulin. J Biol Chem 279:37431-37435
27. Nguyen MT, Satoh H, Favelyukis S, Babendure JL, Imamura T, Sbodio JI, Zalevsky J, Dahiyat BI, Chi NW, Olefsky JM 2005 JNK and tumor necrosis factor-α mediate free fatty acid-induced insulin resistance in 3T3-L1 adipocytes. J Biol Chem 280:35361-35371
28. Calderhead DM, Kitagawa K, Tanner LI, Holman GD, Lienhard GE 1990 Insulin regulation of the two glucose transporters in 3T3-L1 adipocytes. J Biol Chem 265:13801-13808