A modified method for purifying amelanotic melanocytes from human hair follicles

Journal of Dermatology

Volumn 33, Issue 4, 2006, Pages 239-248

  Ma, Hui Jun ^a   Yue, Xue Zhuang ^a   Wang, Da Guang ^a   Li, Cheng Rang ^a   Zhu, Wen Yuan ^a,b  

^a NANJING MEDICAL UNIVERSITY   (China)

^b NONE   (China)

Author keywords

Amelanotic melanocyte; Cell culture; Modified; Outer root sheath

Indexed keywords

ANTIGEN; GLYCOPROTEIN GP 100; LEVODOPA; MITOGENIC AGENT; MONOPHENOL MONOOXYGENASE; POLYCLONAL ANTIBODY; TRYPSIN;

ADULT; ARTICLE; CADAVER; CARBOXY TERMINAL SEQUENCE; CELL CULTURE; CELL PROLIFERATION; CONTROLLED STUDY; CULTURE MEDIUM; CYTOPLASM; DERMIS; ENZYME THERAPY; EPIDERMIS; FEMALE; HAIR FOLLICLE; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; KERATINOCYTE; MALE; MELANOCYTE; MELANOSOME; SCALP; TECHNIQUE; TRANSMISSION ELECTRON MICROSCOPY;

ADULT; CELL CULTURE TECHNIQUES; CELL SEPARATION; FEMALE; HAIR FOLLICLE; HUMANS; MALE; MELANOCYTES;

EID: 33645878895     PISSN: 03852407     EISSN: 13468138     Source Type: Journal    
DOI: 10.1111/j.1346-8138.2006.00059.x     Document Type: Article

References (26)

1. Tobin DJ Bystryn JC Different populations of melanocytes are present in hair follicles and epidermis Pigment Cell Res 1996 9 304 310
2. Arrunategui A Arroyo C Garcia L et al. Melanocyte reservoir in vitiligo Int J Dermatol 1994 33 484 487
3. Takada K Sugiyama K Yamamoto I et al. Presence of amelanotic melanocytes within the outer root sheath in senile white hair J Invest Dermatol 1992 99 629 633
4. Tobin DJ Colen SR Bystryn JC Isolation and long-term culture of human hair follicle melanocytes J Invest Dermatol 1995 104 86 89
5. Zhu WY Zhang RZ Ma HJ et al. Isolation and culture of amelanotic melanocytes from human hair follicle Pigment Cell Res 2004 17 668 673
6. De Luca M Siegrist W Bondanza S et al. Alpha melanocyte stimulating hormone (alpha MSH) stimulates normal human melanocyte growth by binding to high-affinity receptors J Cell Sci 1993 105 1079 1084
7. Krasagakis K Garbe C Kruger-Krasagakes S et al. 12-O-tetradecanoylphorbol-13-acetate not only modulates proliferation rates, but also alters antigen expression and LAK-cell susceptibility of normal human melanocytes in vitro J Invest Dermatol 1993 100 653 659
8. White R Hu F Roman NA False dopa reaction in studies of mammalian tyrosinase: some characteristics and precautions Stain Technol 1983 58 13 19
9. Adema GJ Bakker AB de Boer AJ et al. pMel17 is recognized by monoclonal antibodies NKI-beteb, HMB-45 and HMB-50 and by anti-melanoma CTL Br J Cancer 1996 73 1044 1048
10. Kushimoto T Basrur V Valencia J et al. A model for melanosome biogenesis based on the purification and analysis of early melanosomes Proc Natl Acad Sci U S A 2001 98 10698 10703
11. Raposo G Fevrier B Stoorvogel W et al. Lysosome-related organelles: a view from immunity and pigmentation Cell Struct Funct 2002 27 443 456
12. Cui J Shen LY Wang GC Role of the hair follicles in the repigmentation of vitiligo J Invest Dermatol 1991 97 410 416
13. Horikawa T Norris DA Johnson TW et al. DOPA-negative melanocytes in the outer root sheath of human hair follicles express premelanosomal antigens but not a melanosomal antigen or the melanosome-associated glycoproteins tyrosinase, TRP-1, and TRP-2 J Invest Dermatol 1996 106 28 35
14. Ugele B Lange F Isolation of endothelial cells from human placental microvessels: effect of different proteolytic enzymes on releasing endothelial cells from villous tissue In Vitro Cell Dev Biol Anim 2001 37 408 413
15. Belles-Isles M Roy R Dansereau G et al. Rapid selection of donor myoblast clones for muscular dystrophy therapy using cell surface expression of NCAM Eur J Histochem 1993 37 375 380
16. Hu XT Xiong LJ Lao J The methodological study of schwann cell culture from sciatic nerver of adult SD rat Zhonghua xian wei wai ke za zhi 2002 25 197 200 Chinese
17. Le Poole IC Boyce ST Keratinocytes suppress transforming growth factor-beta1 expression by fibroblasts in cultured skin substitutes Br J Dermatol 1999 140 409 416
18. Cook AL Donatien PD Smith AG et al. Human melanoblasts in culture: expression of BRN2 and synergistic regulation by fibroblast growth factor-2, stem cell factor, and endothelin-3 J Invest Dermatol 2003 121 1150 1159
19. Ha laban R Langdon R Birchall N Basic fibroblast growth factor from human keratinocytes is a natural mitogen for melanocytes J Cell Biol 1988 107 1611 1619
20. Sviderskaya EV Wakeling WF Bennett DC A cloned, immortal line of murine melanoblasts inducible to differentiate to melanocytes Development 1995 121 1547 1557
21. Yaar M Eller MS DiBenedetto P et al. The trk family of receptors mediates nerve growth factor and neurotrophin-3 effects in melanocytes J Clin Invest 1994 94 1550 1562
22. Sieber-Blum M Growth factor synergism and antagonism in early neural crest development Biochem Cell Biol 1998 76 1039 1050
23. Hirobe T Endothelins are involved in regulating the proliferation and differentiation of mouse epidermal melanocytes in serum-free primary culture J Investig Dermatol Symp Proc 2001 6 25 31
24. Ya da Y Higuchi K Imokawa G Effects of endothelins on signal transduction and proliferation in human melanocytes J Biol Chem 1991 266 18352 18357
25. Morrison SJ Csete M Groves AK et al. Culture in reduced levels of oxygen promotes clonogenic sympathoadrenal differentiation by isolated neural crest stem cells J Neurosci 2001 20 7370 7376
26. Blancher C Harris AL The molecular basis of the hypoxia response pathway: tumour hypoxia as a therapy target Cancer Metastasis Rev 1998 17 187 194