Purification of His6-organophosphate hydrolase using monolithic supermacroporous polyacrylamide cryogels developed for immobilized metal affinity chromatography

Applied Microbiology and Biotechnology

Volumn 70, Issue 5, 2006, Pages 558-563

  Efremenko, E ^a   Votchitseva, Y ^a   Plieva, F ^b,c   Galaev, I ^c   Mattiasson, B ^c  

^a MOSCOW STATE UNIVERSITY   (Russian Federation)

^b Ideon Science Park   (Sweden)

^c LUND UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

ADSORBENTS; AMINATION; CELLS; CHROMATOGRAPHY; ENZYMES; ESCHERICHIA COLI; ORGANIC ACIDS; ORGANIC COMPOUNDS; PROTEINS;

ETHYLENDIAMINE; IMINODIACETIC ACID; METAL AFFINITY CHROMATOGRAPHY; MONOLITHIC SUPERMACROPOROUS POLYACRYLAMIDE CRYOGELS;

PHOSPHATES;

AGAROSE; BUFFER; DIGLYCINE; ENZYME; HISTIDINE; HYDROLASE; IMIDAZOLE; METAL; METAL ION; NITRILOTRIACETIC ACID; ORGANOPHOSPHATE HYDROLASE; POLYACRYLAMIDE; UNCLASSIFIED DRUG; ACRYLIC ACID RESIN; CRYOGEL; FIBRONECTIN; IMINO ACID; PHOSPHATASE; PHOSPHORYLPHOSPHATASE; PLASMA PROTEIN; POLYACRYLAMIDE GELS; RECOMBINANT PROTEIN;

ACETIC ACID; CHROMATOGRAPHY; ENZYME; ENZYME ACTIVITY; REGENERATION; TAGGING;

AFFINITY CHROMATOGRAPHY; ARTICLE; CELL FREE SYSTEM; COMPARATIVE ANATOMY; ENZYME PURIFICATION; ESCHERICHIA COLI; FLOW RATE; NONHUMAN; POROSITY; PROTEIN EXPRESSION; PURIFICATION; ULTRASOUND; BINDING SITE; CHEMISTRY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY;

ESCHERICHIA COLI;

ACRYLIC RESINS; BINDING SITES; BLOOD PROTEINS; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; FIBRONECTINS; IMINO ACIDS; METALS; PHOSPHORIC MONOESTER HYDROLASES; RECOMBINANT PROTEINS;

EID: 33645708122     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-005-0103-x     Document Type: Article

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