Construction of a reporter strain Pseudomonas putida for the detection of oxidative stress caused by environmental pollutants

Journal of Microbiology and Biotechnology

Volumn 16, Issue 3, 2006, Pages 386-390

  Lee, Yunho ^a   Ahn, Eunyoung ^b   Park, Sungsu ^b   Madsen, Eugene L ^c   Jeon, Che Ok ^d   Park, Woojun ^a,d  

^a Korea University   (South Korea)

^b Ewha Womans University   (South Korea)

^c Department of Molecular Biology and Genetics   (United States)

^d Gyeongsang National University   (South Korea)

Author keywords

Green fluorescence protein; Hydrogen peroxide; Menadione; Paint thinner; Paraquat

Indexed keywords

FERREDOXIN NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE REDUCTASE; GASOLINE; GREEN FLUORESCENT PROTEIN; HYDROGEN PEROXIDE; MENADIONE; PARAQUAT; THINNER; TOLUENE;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; CONTROLLED STUDY; ENZYME INDUCTION; GENE CONSTRUCT; GENE EXPRESSION; GENE VECTOR; HOST RANGE; NONHUMAN; NORTHERN BLOTTING; OXIDATIVE STRESS; PLASMID; POLLUTANT; PROMOTER REGION; PSEUDOMONAS PUTIDA; REPORTER GENE; WHOLE CELL;

BACTERIA (MICROORGANISMS); PSEUDOMONAS PUTIDA;

EID: 33645548032     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (28)

1. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struthl. 1999. Current Protocols in Molecular Biology. John Wiley & Sons, Inc., New York, N.Y, U.S.A.
2. Baik, S. H., P. K. Cho, M. H. Kim, and S. F. Yun. 2003. Enhanced production of benzoylformate reductase in Enterococcus faecalis under oxidative stress established by natural electron carriers. J. Microbiol. Biotechnol. 13: 104-109.
3. Belkin, S. 2003. Microbial whole-cell sensing systems of environmental pollutants. Curr. Opin. Microbiol. 6: 206-212.
4. Bianchi, V., E. Haggard-Ljungquist, E. Pontis, and P. Reichard. 1995. Interruption of the ferredoxin (flavodoxin) NADP+ oxidoreductase gene of Escherichia coli does not affect anaerobic growth but increases sensitivity to paraquat. J. Bacteriol. 177: 4528-4531.
5. Chavez, F. P., H. Lunsdorf, and C. A. Jerez. 2004. Growth of polychlorinated-biphenyl-degrading bacteria in the presence of biphenyl and chlorobiphenyls generates oxidative stress and massive accumulation of inorganic polyphosphate. Appl. Environ. Microbiol. 70: 3064-3072.
6. Halliwell, B. and J. M. C. Gutteridge. 1999. Free Radicals in Biology and Medicine. The 3rd ed. Oxford Press, Oxford.
7. Hidalgo, F., V. Leautaud, and B. Demple. 1998. The redox-regulated SoxR protein acts from a single DNA site as a repressor and an allosteric activator. EMBO J. 17: 2629-2636.
8. Imlay, J.A. 2003. Pathways of oxidative damage. Annu. Rev. Microbiol. 57: 395-418.
9. Justus, T. and S. M. Thomas. 1999. Evaluation of transcriptional fusions with green fluorescent protein versus luciferase as reporters in bacterial mutagenicity tests. Mutagenesis 14: 351-356.
10. Keane, A., P. Phoenix, S. Ghoshal, and P. C. Lau. 2002. Exposing culprit organic pollutants: A review. J. Microbiol. Methods 49: 103-119.
11. Kim, I. M., Y. C. Lee, J. S. Won, and M. H. Choe. 2003. Identification of genes for growth with oxygen in Escherichia coli by operon fusion and Southern blot techniques. J. Microbiol. Biotechnol. 13: 976-983.
12. Kim, J. S., J. H. Kim, F. K. Ryu, J. K. Kim, C. K. Kim, I. G. Hwang, and K. Lee. 2004. Versatile catabolic properties of Tn4371-encoded bph pathway in Comamonas testosteroni (formerly Pseudomonas sp.) NCIMB 10643. J. Microbiol. Biotechnol. 14: 302-311.
13. Kim, T. K., H. D. Shin, M. C. Seo, J. N. Lee, and Y. H. Lee. 2003. Molecular structure of PCR cloned PHA synthase genes of Pseudomonas putida KT2440 and its utilization for medium-chain length polyhydroxyalkanoate production. J. Microbiol. Biotechnol. 13: 182-190.
14. Kim, Y. C., C. S. Kim, B. H. Cho, and A. J. Anderson. 2004. Major Fe-superoxide dismutase (FeSOD) activity in Pseudomonas putida is essential for survival under conditions of oxidative stress during microbial challenge and nutrient limitation. J. Microbiol. Biotechnol. 14: 859-862.
15. Krapp, A. R., R. E. Rodriguez, H. O. Poli, D. H. Paladini, J. F. Palatnik, and N. Carrillo. 2002. The flavoenzyme ferredoxin (flavodoxin)-NADP(H) reductase modulates NADP(H) homeostasis during the soxRS response of Escherichia coli. J. Bacteriol. 184: 1474-1480.
16. Krayl, M., D. Benndorf, N. Loffhagen, and W. Babel. 2003. Use of proteomics and physiological characteristics to elucidate ecotoxic effects of methyl tert-butyl ether in Pseudomonas putida KT2440. Proteomics 3: 1544-1552.
17. Min J. and M. B. Ku. 2004. Adaptive responses of Escherichia coli for oxidative and protein damage using bioluminescence reporters. J. Microbiol. Biotechnol. 14: 466-469.
18. Mostertz, J., C. Scharf, M. Hecker, and G. Homuth. 2004. Transcriptome and proteome analysis of Bacillus subtilis gene expression in response to superoxide and peroxide stress. Microbiology 150: 497-512.
19. Nunoshiba, T., E. Hidalgo, C. F. Amabile Cuevas, and B. Demple. 1992. Two-stage control of an oxidative stress regulon: The Escherichia coli SoxR protein triggers redox-inducible expression of the soxS regulatory gene. J. Bacteriol. 174: 6054-6060.
20. Park, W., C. O. Jean, A. M. Hohnstock-Ashe, S. C. Winans, G. J. Zylstra, and E. L. Madsen. 2003. Identification and characterization of the conjugal transfer region of the pCg1 plasmid from naphthalene-degrading Pseudomonas putida Cg1. Appl. Environ. Microbiol. 69: 3263-3271.
21. Pomposiello, P. J. and B. Demple. 2001. Redox-operated genetic switches: The SoxR and OxyR transcription factors. Trends Biotechnol. 19: 109-114.
22. Schweigert, N., S. Belkin, P. Leong-Morgenthaler, A. J. Zehnder, and R. I. Eggen. 1999. Combinations of chlorocatechols and heavy metals cause DNA degradation in vitro but must not result in increased mutation rates in vivo. Environ. Mol. Mutagen. 33: 202-210.
23. Simon, R., U. Priefer, and A. Puhler. 1983. A broad hostrange mobilization system for in vivo genetic engineering: Transposon mutagenesis in Gram-negative bacteria. Bio/Technology 1: 784-791.
24. Smith, A. H., J. A. Imlay, and R. I. Mackie. 2003. Increasing the oxidative stress response allows Escherichia coli to overcome inhibitory effects of condensed tannins. Appl. Environ. Microbiol. 69: 3406-3411.
25. Storz, G. and J. A. Imaly. 1999. Oxidative stress. Curr. Opin. Microbiol. 2: 188-194.
26. Yin, S., M. Fuangthong, W. P. Laratta, and J. P. Shapleigh. 2003. Use of a green fluorescent protein-based reporter fusion for detection of nitric oxide produced by denitrifiers. Appl. Environ. Microbiol. 69: 3938-3944.
27. Zheng, M. and G. Storz, 2000. Redox sensing by prokaryotic transcription factors. Biochem. Pharmacol. 59: 1-6.
28. Zheng, M., X. Wang, L. J. Templeton, D. R Smulski, R. A. LaRossa, and G. Storz. 2001. DNA microarray-mediated transcriptional profiling of the Escherichia coli response to hydrogen peroxide. J. Bacteriol. 183: 4562-4570.