Spectral characterization of Dictyostelium autofluorescence

Microscopy Research and Technique

Volumn 69, Issue 3, 2006, Pages 168-174

  Engel, Ruchira ^a   Van Haastert, Peter J M ^b   Visser, Antonie J W G ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

Chemotaxis; Confocal microscopy; Fluorescence fluctuation spectroscopy; Spectral imaging

Indexed keywords

BIOCHEMISTRY; CELLS; CONFOCAL MICROSCOPY; CYTOLOGY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; LUMINANCE; PHOTOBLEACHING; PROTEINS; SPECTRUM ANALYZERS;

AUTOFLUORESCENCE; CELL-BE; CELL/B.E; CELL/BE; CELLULARS; CHEMOTAXI; DICTYOSTELIUM; FLUORESCENCE FLUCTUATION SPECTROSCOPY; SPECTRAL CHARACTERIZATION; SPECTRAL IMAGING;

SPATIAL DISTRIBUTION;

BUFFER; ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN;

ARTICLE; AUTOFLUORESCENCE; BRIGHTNESS; CHEMOTAXIS; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DICTYOSTELIUM DISCOIDEUM; FLUORESCENCE; FLUORESCENCE FLUCTUATION SPECTROSCOPY; FLUORESCENCE MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; SPECTRAL SENSITIVITY; TECHNIQUE;

DICTYOSTELIUM; DICTYOSTELIUM DISCOIDEUM;

EID: 33645508585     PISSN: 1059910X     EISSN: 10970029     Source Type: Journal    
DOI: 10.1002/jemt.20282     Document Type: Article

References (31)

1. Aguado-Velasco C, Bretscher MS. 1999. Circulation of the plasma membrane in Dictyostelium. Mol Biol Cell 10:4419-4427.
2. Aubin J. 1979. Autofluorescence of viable cultured mammalian cells. J Histochem Cytochem 27:36-43.
3. Benson R, Meyer R, Zaruba M, McKhann G. 1979. Cellular autofluorescence-is it due to flavins? J Histochem Cytochem 27:44-48.
4. Blaauw M, Knol JC, Kortholt A, Roelofs J, Ruchira, Postma M, Visser AJWG, Van Haastert PJM. 2003. Phosducin-like proteins in Dictyostelium discoideum: implications for the phosducin family of proteins. EMBO J 22:5047-5057.
5. Bretschneider T, Jonkman J, Kohler J, Medalia O, Barisic K, Weber I, Stelzer EH, Baumeister W, Gerisch G. 2002. Dynamic organization of the actin system in the motile cells of Dictyostelium. J Muscle Res Cell Motil 23:639-649.
6. Brock R, Hink MA, Jovin TM. 1998. Fluorescence correlation microscopy of cells in the presence of autofluorescence. Biophys J 75:2547-2557.
7. Brock R, Vamosi G, Vereb G, Jovin TM. 1999. Rapid characterization of green fluorescent protein fusion proteins on the molecular and cellular level by fluorescence correlation microscopy. Proc Natl Acad Sci USA 96:10123-10128.
8. Chen Y, Müller JD, So PT, Gratton E. 1999. The photon counting histogram in fluorescence fluctuation spectroscopy. Biophys J 77:553-567.
9. Chen Y, Müller JD, Ruan Q, Gratton E. 2002. Molecular brightness characterization of eGFP in vivo by fluorescence fluctuation spectroscopy. Biophys J 82:133-144.
10. Fischer M, Haase I, Simmeth E, Gerisch G, Muller-Taubenberger A. 2004. A brilliant monomeric red fluorescent protein to visualize cytoskeleton dynamics in Dictyostelium. FEBS Lett 577:227-232.
11. Haustein E, Schwille P. 2003. Ultrasensitive investigations of biological systems by fluorescence correlation spectroscopy. Methods 29:153-166.
12. Hess ST, Huang S, Heikal AA, Webb WW. 2002. Biological and chemical applications of fluorescence correlation spectroscopy: a review. Biochemistry 41:697-705.
13. Hink MA, Borst JW, Visser AJ. 2003. Fluorescence correlation spectroscopy of GFP fusion proteins in living plant cells. Methods Enzymol 361:93-112.
14. Janetopoulos C, Jin T, Devreotes PN. 2001. Receptor-mediated activation of heterotrimeric G-proteins in living cells. Science 291:2408-2411.
15. Jin T, Zhang N, Long Y, Parent CA, Devreotes PN. 2000. Localization of the G protein βγ complex in living cells during chemotaxis. Science 287:1034-1036.
16. Kayman S, Clarke M. 1983. Relationship between axenic growth of Dictyostelium discoideum strains and their track morphology on substrates coated with gold particles. J Cell Biol 97:1001-1010.
17. Kriebel PW, Barr VA, Parent CA. 2003. Adenylyl cyclase localization regulates streaming during chemotaxis. Cell 112:549-560.
18. Levin MK, Carson JH. 2004. Fluorescence correlation spectroscopy and quantitative cell biology. Differentiation 72:1-10.
19. Parent CA, Blacklock BJ, Froehlich WM, Murphy DB, Devreotes PN. 1998. G-protein signaling events are activated at the leading edge of chemotactic cells. Cell 95:81-91.
20. Potma EO, de Boeij WP, Bosgraaf L, Roelofs J, Van Haastert PJ, Wiersma DA. 2001. Reduced protein diffusion rate by cytoskeleton in vegetative and polarized Dictyostelium cells. Biophys J 81:2010-2019.
21. Ruchira , Hink MA, Bosgraaf L, Van Haastert PJM, Visser AJWG. 2004. Pleckstrin homology domain diffusion in Dictyostelium cytoplasm studied using fluorescence correlation spectroscopy. J Biol Chem 279:10013-10019.
22. Schwille P, Meyer-Almes FJ, Rigler R. 1997. Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution. Biophys J 72:1878-1886.
23. Shaner NC, Campbell RE, Steinbach PA, Giepmans BNG, Palmer AE, Tsien RY. 2004. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat Biotechnol 22:1567-1572.
24. Skakun VV, Hink MA, Digris AV, Engel R, Novikov EG, Apanasovich W, Visser AJ. 2005. Global analysis of fluorescence fluctuation data. Eur Biophys J 34:323-334.
25. Tsien RY. 1998. The green fluorescent protein. Annu Rev Biochem 67:509-544.
26. Uchida KSK, Yumura S. 2004. Dynamics of novel feet of Dictyostelium cells during migration. J Cell Sci 117:1443-1455.
27. Veltman DM, Roelofs J, Engel R, Visser AJWG, Van Haastert PJM. 2005. Activation of soluble guanylyl cyclase at the leading edge during Dictyostelium chemotaxis. Mol Biol Cell 16:976-983.
28. Visser AJWG, vdBerg PAW, Hink MA, Petushkov VN. 2001. Fluorescence correlation spectroscopy of flavins and flavoproteins. In: Rigler R, Elson ES, editors. Fluorescence correlation spectroscopy: theory and applications. Berlin: Springer-Verlag, pp 9-24.
29. Vukojevic V, Pramanik A, Yakovleva T, Rigler R, Terenius L, Bakalkin G. 2005. Study of molecular events in cells by fluorescence correlation spectroscopy. Cell Mol Life Sci 62:535-550.
30. Widengren J. 2001. Photophysical aspects of FCS measurements. In: Rigler R, Elson ES, editors. Fluorescence correlation spectroscopy: theory and applications. Berlin: Springer-Verlag, pp 276-301.
31. Xu X, Meier-Schellersheim M, Jiao X, Nelson LE, Jin T. 2005. Quantitative imaging of single live cells reveals spatiotemporal dynamics of multistep signaling events of chemoattractant gradient sensing in Dictyostelium. Mol Biol Cell 16:676-688.