The antiatherogenic effect of allicin: Possible mode of action

Pathobiology

Volumn 72, Issue 6, 2006, Pages 325-334

  Gonen, Ayelet ^a,b   Harats, Dror ^a,b   Rabinkov, Aharon ^c   Miron, Talia ^c   Mirelman, David ^c   Wilchek, Meir ^c   Weiner, Lev ^c   Ulman, Esfir ^a   Levkovitz, Hana ^a   Ben Shushan, Dikla ^a,b   Shaish, Aviv ^a  

^a SHEBA MEDICAL CENTER   (Israel)

^b TEL AVIV UNIVERSITY   (Israel)

^c WEIZMANN INSTITUTE OF SCIENCE   (Israel)

Author keywords

Allicin; Apolipoprotein E deficient mice; Atherosclerosis; Lipoproteins; Low density lipoprotein receptor knockout mice

Indexed keywords

ALLICIN; APOLIPOPROTEIN E; COPPER ION; COPPER SULFATE; LIPOPROTEIN; LOW DENSITY LIPOPROTEIN; LOW DENSITY LIPOPROTEIN RECEPTOR; TRITIUM;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIOXIDANT ACTIVITY; ARTICLE; ATHEROGENESIS; ATHEROSCLEROSIS; ATHEROSCLEROTIC PLAQUE; BODY WEIGHT; CARDIOVASCULAR RISK; CONTROLLED STUDY; DIET SUPPLEMENTATION; DRUG MECHANISM; DRUG PROTEIN BINDING; ELECTRON SPIN RESONANCE; FEMALE; GARLIC; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; KNOCKOUT MOUSE; LIPID DEGRADATION; LIPID OXIDATION; LIPID TRANSPORT; MOUSE; NONHUMAN; NORMAL HUMAN; PERITONEUM MACROPHAGE; PRIORITY JOURNAL;

ANIMALS; ANTILIPEMIC AGENTS; ANTIOXIDANTS; AORTA; APOLIPOPROTEINS E; ARTERIOSCLEROSIS; CHOLESTEROL; CHROMATOGRAPHY, LIQUID; ELECTRON SPIN RESONANCE SPECTROSCOPY; HUMANS; LIPOPROTEINS, LDL; MACROPHAGES, PERITONEAL; MICE; MICE, KNOCKOUT; OXIDATION-REDUCTION; RECEPTORS, LDL; SULFINIC ACIDS;

ALLIUM SATIVUM; ANIMALIA; SYZYGIUM AROMATICUM;

EID: 33645470194     PISSN: 10152008     EISSN: None     Source Type: Journal    
DOI: 10.1159/000091330     Document Type: Article

References (51)

1. Lusis AJ: Atherosclerosis. Nature 2000;407:233-241.
2. Glass CK, Witztum JL: Atherosclerosis: the road ahead. Cell 2001;104:503-516.
3. Stocker R, Keaney JF: Role of oxidative modifications in atherosclerosis. Physiol Rev 2004;84:1381-1478.
4. Parthasarathy S, Santanam N, Ramachandran S, Meilhac O: Oxidants and antioxidants in atherogenesis. An appraisal. J Lipid Res 1999;40:2143-2157.
5. Diaz MN, Frei B, Vita JA, Keaney JF: Antioxidants and atherosclerotic heart disease. N Engl J Med 1997;337:408-416.
6. Ferguson E, Singh RJ, Hogg N, Kalyanaraman B: The mechanism of apolipoprotein B-100 thiol depletion during oxidative modification of low density lipoprotein. Arch Biochem Biophys 1997;341:287-294.
7. Roland A, Patterson RA, Leake DS: Measurement of copper-binding sites on low density lipoprotein. Arterioscler Thromb Vasc Biol 2001;21:594-602.
8. Jessup W, Kritharides L: Metabolism of oxidized LDL by macrophages. Curr Opin Lipidol 2000;11:473-481.
9. Wang X, Greilberger J, Ledinski G, Kager G, Jurgens G: Binding and uptake of differently oxidized low density lipoprotein in mouse peritoneal macrophages and THP-1 macrophages: involvement of negative charges as well as oxidation-specific epitopes. J Cell Biochem 2001;81:557-569.
10. Chait A, Heinecke JW: Lipoprotein modification: cellular mechanisms. Curr Opin Lipidol 1994;5:365-370.
11. Burkitt MJ: A critical overview of the chemistry of copper-dependent low density lipoprotein oxidation: roles of lipid hydroperoxides, alpha-tocopherol, thiols, and cerulo-plasmin. Arch Biochem Biophys 2001;394:117-135.
12. Ackermann R, Mulrow C, Ramirez G, Gardner C, Morbidoni L, Lawrence V: Garlic shows promise for improving some cardiovascular risk factors. Arch Intern Med 2001;161:813-824.
13. Imai J, Ide N, Nagae S, Moriguchi T, Matsuura H, Itakura Y: Antioxidant and radical scavenging effects of aged garlic extract and its constituents. Planta Med 1994;60:417-420.
14. Ali M, Al-Qattan KK, Al-Enezi F, Khanafer RM, Mustafa T: Effect of allicin from garlic powder on serum lipids and blood pressure in rats fed with a high cholesterol diet. Prostaglandins Leukot Essent Fatty Acids 2000;62:253-259.
15. Campbell JH, Efendy JL, Smith NJ, Campbell GR: Molecular basis by which garlic suppresses atherosclerosis. J Nutr 2001;131:1006S-1009S.
16. Eilat S, Oestraicher Y, Rabinkov A, Ohad D, Mirelman D, Battler A, Eldar M, Vered Z: Alteration of lipid profile in hyperlipidemic rabbits by allicin, an active constituent of garlic. Coronary Artery Dis 1995;6:985-990.
17. Kwon MJ, Song YS, Choi MS, Park SJ, Jeong KS, Song YO: Cholesteryl ester transfer protein activity and atherogenic parameters in rabbits supplemented with cholesterol and garlic powder. Life Sci 2003;72:2953-2964.
18. Munday JS, James KA, Fray LM, Kirkwood SW, Thompson KG: Daily supplementation with aged garlic extract, but not raw garlic, protects low density lipoprotein against in vitro oxidation. Atherosclerosis 1999;143:399-404.
19. Dillon SA, Burmi RS, Lowe GM, Billington D, Rahman K: Antioxidant properties of aged garlic extract: an in vitro study incorporating human low density lipoprotein. Life Sci 2003;72:2953-2964.
20. Brace LD: Cardiovascular benefits of garlic (Allium sativum L). J Cardiovasc Nurs 2002;16:33-49.
21. Orekhov AN, Grunwald J: Effects of garlic on atherosclerosis. Nutrition 1997;13:656-663.
22. Banerjee SK, Maulik SK: Effect of garlic on cardiovascular disorder: a review. Nutrition J 2002;1:1-14.
23. Efendy JL, Simmons DL, Campbell GR, Campbell JH: The effect of the aged garlic extract, 'Kyolic', on the development of experimental atherosclerosis. Atherosclerosis 1997;132:37-42.
24. Abramovitz D, Gavri S, Harats D, Levkovitz H, Mirelman D, Miron T, Eilat-Adar S, Rabinkov A, Wilchek M, Eldar M, Vered Z: Allicin-induced decrease in formation of fatty steaks (atherosclerosis) in mice fed on a cholesterol-rich diet. Coronary Artery Dis 1999;10:515-519.
25. Prasad K, Laxdal VA, Yu M, Raney BL: Evaluation of hydroxyl radical-scavenging property of garlic. Mol Cell Biochem 1996;154:55-63.
26. Espirito Santo SM, van Vlijmen BJ, Buytenhek R, van Duyvenvoorde W, Havekes LM, Arnault I, Auger J, Princen HM: Well-characterized garlic-derived materials are not hypolipidemic in APOE*3-Leiden transgenic mice. J Nutr 2004;134:1500-1503.
27. Espirito Santo SM, van Vlijmen BJ, van Duyvenvoorde W, Offerman EH, Havekes LM, Arnault I, Auger J, Princen HM: Absence of an atheroprotective effect of the garlic powder printanor in APOE*3-Leiden transgenic mice. Atherosclerosis 2004;177:291-297.
28. Yeh YY, Liu L: Cholesterol-lowering effect of garlic extracts and organosulfur compounds: human and animal studies. J Nutr 2001;131:989S-993S.
29. Lawson LD, Gardner CD: Composition, stability, and bioavailability of garlic products used in a clinical trail. J Agric Food Chem 2005;53:6254-6261.
30. Hsu CC, Huang CN, Hung YC, Yin MC: Five cysteine-containing compounds have antioxidative activity in Balb/cA mice. J Nutr 2004;134:149-152.
31. Rabinkov A, Miron T, Konstantinovski L, Wilchek M, Mirelman D, Weiner L: The mode of action of allicin: trapping of radicals and interaction with thiol containing proteins. Biochim Biophys Acta 1998;1379:233-244.
32. Miron T, Bercovici T, Rabinkov A, Wilchek M, Mirelman D: [3H]Allicin: preparation and applications. Anal Biochem 2004;331:364-369.
33. Hatch FT: Practical methods for plasma lipoprotein analysis. Adv Lipid Res 1968;6:1-68.
34. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with Folin phenol reagent. J Biol Chem 1951;193:265-275.
35. Gieseg SP, Esterbauer H: Low density lipoprotein is saturable by pro-oxidant copper. FEBS Lett 1994;343:188-194.
36. Salacinski PR, McLean C, Sykes JE, Clement-Jones W, Lowry PJ: Iodination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent 1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenyl glycoluril (Iodogen). Anal Biochem 1981;117:136-146.
37. Bellosta S, Mahley RW, Sanan DA, Murata J, Newland DL, Taylor JM, Pitas RE: Macrophage-specific expression of human apolipoprotein E reduces atherosclerosis in hypercholesterolemic apolipoprotein E-null mice. J Clin Invest 1995;96:2170-2179.
38. Goldstein JL, Basu SK, Brown MS: Receptor-mediated endocytosis of low density lipoprotein in cultured cells. Methods Enzymol 1983;98:241-260.
39. Ishibashi S, Herz J, Maeda N, Goldstein JL, Brown MS: The two-receptor model of lipoprotein clearance: tests of the hypothesis in 'knock-out' mice lacking the low density lipoprotein receptor, apolipoprotein E, or both proteins. Proc Natl Acad Sci USA 1994;91:4431-4435.
40. 2+): a study by electron spin resonance (ESR) spectroscopy and viscosimetry. Polym Mat Sci Eng 1999;79:475-476.
41. Millard CB, Shnyrov VL, Newstead S, Shin I, Roth E, Silman I, Weiner L: Stabilization of a metastable state of Torpedo californica acetylcholinesterase by chemical chaperones. Protein Sci 2003;12:2337-2347.
42. Miron T, Rabinkov A, Mirelman D, Wilchek M, Weiner L: The mode of action of allicin: its ready permeability through phospholipid membranes may contribute to its biological activity. Biochim Biophys Acta 2000;1463:20-30.
43. Eliash T, Weiner L, Ottolenghi M, Sheves M: Specific binding sites for cations in bacteriorhodopsin. Biophys J 2001;81:1155-1162.
44. Aharoni A, Weiner L, Ottolenghi M, Sheves M: Bacteriorhodopsin experiences light-induced conformational alterations in nonisomerizable C(13) = C(14) pigments. A study with EPR. J Biol Chem 2000;275:21010-21016.
45. Veniant MM, Withycombe S, Young SG: Lipoprotein size and atherosclerosis susceptibility in Apoe(-/-) and LDL-R(-/-) mice. Arterioscler Thromb Vasc Biol 2001;21:1567-1570.
46. Lawson DL, Wang ZJ: Allicin release under simulated gastrointestinal conditions from garlic powder tablets employed in clinical trials on serum cholesterol. Planta Med 2001;67:13-18.
47. Lebedev VS, Volodina LA, Fedorov I: Analysis of the binding site of copper with intact and Escherichia coli bacteria modified by N-ethylmaleimide by ESR. Biofizika 1991;36:91-96.
48. Hong SY, Yang JO, Lee EY, Lee ZW: Effects of N-acetyl-L-cystein and glutathion on antioxidant status of human serum and 3T3 fibroblasts. J Korean Med Sci 2003;18:649-654.
49. Gruarin P, Sitia R, Alessio M: Formation of one or more intrachain disulphide bonds is required for the intracellular processing and transport of CD36. Biochem J 1997;328:635-642.
50. Lawson LD, Wang ZJ: Allicin and allicin-derived garlic compounds increase breath acetone through allyl methyl sulfide: use in measuring allicin bioavailability. J Agric Food Chem 2005;53:1974-1983.
51. Rosen RT, Hiserodt RD, Fukuda EK, Ruiz RJ, Zhou Z, Lech J, Rosen SL, Hartman TG: Determination of allicin, S-allylcysteine and volatile metabolites of garlic in breath, plasma or simulated gastric fluids. J Nutr 2001;131:968S-971S.