Immunological and biomedical relevance of the Tn antigen

Inmunologia

Volumn 22, Issue 1, 2003, Pages 27-38

  Freire, T ^a   Osinaga, Eduardo ^a  

^a DEPARTAMENTO DE BIOQUÍMICA   (Uruguay)

Author keywords

Glycosyltransferases; O glycosylation; Tn antigen; Tumour associated antigens

Indexed keywords

EID: 33645462477     PISSN: 02139626     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (109)

1. Moreau R, Dausset, J, Bernard J, Moullec J. Anémie hémolytique acquise avec polyagglutinabilité des hématies par un nouveau facteur present dans le serum humain normal (anti-Tn). Bull Soc Méd Hôp Paris 1957; 73569-587.
2. Dausset J, Moullec J, Bernard J. Acquired hemolytic anemia with polyagglutinability of red blood cells due to a new factor present in normal human serum (anti-Tn). Blood 1959; 14: 1079-93.
3. Friedenreich V. The Thomsen hemagglutination phenomenon. Levin and Munksgaard, Copenhagen, 1930.
4. Dahr W, Uhlenbruck G, Gunson H, van der Hart M. Molecular basis of Tn-polyagglutinability. Vox Sang 1975; 29: 36-50.
5. Springer G, Tegtmeyer H. Origin of anti-Thomsen-Friedenreich (T) and Tn agglutinins in man and in White Leghorn chicks. Br J Haematol 1981; 47: 453-60. (Pubitemid 12131643)
6. Springer G. T and Tn, general carcinoma autoantigens. Science 1984; 224: 1198-206.
7. Hakomori S. Aberrant glycosylation in tumors and tumor-associated carbohydrate antigens. Adv Cancer Res 1989; 52: 257-31.
8. Danishefsky SJ, Allen JR. From the laboratory to the clinic: A retrospective on fully synthetic carbohydrate-based anticancer vaccines. Angew Chem Int Ed Engl 2000; 39: 837-63.
9. van den Steen P, Rudd P, Dwek R, Opdenakker G. Concepts and principles of O-linked glycosylation. Crit Rev Biochem Mol Biol 1998; 33: 151-208.
10. Hanisch FG. O-glycosylation of the mucin type. Biol Chem 2001; 382: 143-9.
11. Hounsell EF, Davies MJ, Renouf DV. O-linked protein glycosylation structure and function. Glycoconjugate J 1996; 13: 19-26. (Pubitemid 26074297)
12. Brockhausen I. Pathways of O-glycan biosynthesis in cancer cells. Biochim Biophys Acta 1999; 1473: 67-95.
13. Clausen H, Bennett E. A family of UDP-GalNAc:polypeptide N-acetylgalactosaminyl- transferases control the initiation of mucintype O-linked glycosylation. Glycobiology 1996; 6: 635-46. (Pubitemid 26386377)
14. Hassan H, Bennett EP, Mandel U, Hollingsworth MA, Clausen H. In: Ernst B, Hart BW, Sinay P (eds). Carbohydrates in Chemistry and Biology. Wiley-VCH, New York, 2000: 271-292.
15. Bill R, Revers L, Wilson I. And what about O-linked sugars? In Protein Glycosylation. Kluwer Academic Publishers, Massachusetts, USA. 1988:281-327.
16. Bird G, Shinton N, Wingham J. Persistent mixed-field polyagglutination. Br J Haematol 1971; 21: 443-53.
17. Berger EG. Tn-syndrome. Biochim Biophys Acta 1999; 1455: 255-68.
18. Thurnher M, Rusconi S, Berger EG. Persistent repression of a functional allele can be responsible for galactosyltransferase deficiency in Tn syndrome. J Clin Invest 1993; 91: 2103-10. (Pubitemid 23141484)
19. Felner K, Dinter A, Cartron J-P, Berger EG. Repressed b-1,3-galactosyltransferase in the Tn syndrome. Biochim Biophys Acta 1998;1406:115-25.
20. Kim YS, Gum J, Brockhausen I. Mucin glycoproteins in neoplasia. Glicoconj J 1996; 13: 693-707.
21. Moniaux N, Escande F, Porchet N, Aubert JP, Batra SK. Structural organization and classification of the human mucin genes. Front Biosci 2001; 6: D1192-206.
22. Brockhausen I, Khun W. Glycosylation in leukemias and blood-related disorders. In: Brockhausen I, Khun W (eds). Glycoproteins and human disease. Springer-Verlag, Heidelberg, Germany, 1998:109-120.
23. Medina M, Velez D, Asenjo JA, Egea G, Real FX, Gil J, Subiza JL. Human colon adenocarcinomas express a MUC1-associated novel carbohydrate epitope on core mucin glycans defined by a monoclonal antibody (A10) raised against murine Ehrlich tumor cells. Cancer Res 1999; 59: 1061-70.
24. Meichenin M, Rocher J, Galanina O, Bovin N, Nifantev N, Sherman A, et al. Tk, a new colon tumor-associated antigen resulting from altered O-glycosylation. Cancer Res 2000; 60: 5499-507.
25. King MJ. Blood group antigens on human erythrocytes . Distribution, structure and possible functions. Biochim Biophys Acta 1994, 1197: 15-44.
26. Doinel C, Andrew G, Cartron JP, Salmon C, Fukuda M. Tk polyagglutination produced in vitro by an endo-b-galactosidase. Vox Sang 1980; 38: 94-8.
27. Terasawa K, Furumoto H, Kamada M, Aono T. Expression of Tn and sialyl-Tn antigens in neoplastic transformation of uterine cervical epithelial cells. Cancer Res 1996; 56: 2229-32. (Pubitemid 26119930)
28. Itzkowitz SH, Bloom EJ, Lau TS, Kim YS. Mucin associated Tn and sialosyl-Tn antigen expression in colorectal polyps. Gut 1992; 33: 518-23.
29. Babino A, Oppezzo P, Bianco S, Barrios E, Berois N, Navarrete H, Osinaga E. Tn antigen is a pre-cancerous biomarker in breast tissue and serum in N-nitrosomethylurea-induced rat mammary carcinogenesis. Int J Cancer 2000; 86:753-9. (Pubitemid 30350188)
30. Hirao T, Sakamoto Y, Kamada M, Hamada S-I, Aono T. Tn antigen, a marker of potential for metastasis of uterine cervix cancer cells. Cancer 1993; 72: 154-9. (Pubitemid 23182066)
31. Springer G. Immunoreactive T and Tn epitopes in cancer diagnosis, prognosis, and immunotherapy. J Mol Med 1997; 75: 594-602. (Pubitemid 27373602)
32. Tsuchiya A, Kanno M, Kawaguchi T, Endo Y, Zhang GJ, Ohtake T, Kimijima I. Prognostic relevance of Tn expression in breast cancer. Breast Cancer 1999; 6: 175-80.
33. Orntoft T, Vestergaard EM. Clinical aspects of altered glycosylation of glycoproteinsin cancer. Electrophoresis 1999; 20: 362-71. (Pubitemid 29125024)
34. Schlepper-Schäfer J, Springer G. Carcinoma auto-antigens T and Tn and their cleavage products interact with Gal/GalNAc-specific receptors on rat Kupffer cells and hepatocytes. Biochem Biophys Acta 1989; 1013: 266-72. (Pubitemid 19240322)
35. Susuki N, Yamamoto K, Toyoshima S, Osawa T, Irimura T. Molecular cloning and expression of cDNA encoding human macrophage C-type lectin. Its unique binding specificity for Tn antigen. J Immunol 1996; 156: 12835.
36. Springer G, Cheingsong-Popov R, Schirrmacher V, Desai P, Tegtmeyer H. Proposed molecular basis of murine tumor cell-hepatocyte interaction. J Biol Chem 1983; 258: 5702-6.
37. Glinsky VV, Glinsky GV, Rittenhouse-Olson K, Huflejt ME, Glinskii OV, Deutscher SL, Quinn TP. The role of Thomsen-Friedenreich antigen in adhesion of human breast and prostate cancer cells to the endothelium. Cancer Res 2001; 61: 48517. (Pubitemid 32691901)
38. Beuzelin-Yvraut M, Bourguignat A, Phippips E, Roseto A, Osinaga E. Immunocytological analysis of the Tn-associated antigen (83D4) in serous effusions from patients with cancer: comparison with Tn soluble glycoprotein. J Clin Pathol 1995; 48: 433-7.
39. Davidson B, Berner A, Nesland J, Risberg B, Kristensen G, Tropé C, Bryne M. Carbohydrate antigen expression in primary tumors, metastatic lesions, and serous effusions from patients diagnosed with epithelial ovarian carcinoma: evidence of upregulated Tn and sialyl-Tn antigen expression in effusions. Hum Pathol 2000; 31: 1081-7.
40. Osinaga E, Pancino G, Beuzelin M, Babino A, Rodriguez D, Robello C, et al. Detection of a soluble antigen defined by monoclonal antibody 83D4 in serous effusions associated with breast carcinoma. Cancer 1992; 69: 1745-9.
41. Osinaga E, Babino A, Grosclaude J, Cairoli E, Batthyany C, Bianchi S, et al. Development of an immuno-lectin-enzymatic assay for the detection of serum carcinoma-associated glycoproteins bearing Tn determinant. Int J Oncol 1996; 8: 401-6. (Pubitemid 26047274)
42. Charpin C, Pancino G, Osinaga E, Bonnier P, Lavaut MN, Allasia C, Roseto A. Monoclonal antibody 83D4 immunoreactivity in human tissues: cellular distribution, and microcytophotometric analysis of immunoprecipitates on tissue sections. Anticancer Res 1992 ; 12: 209-24.
43. Springer G, Taylor C, Howard D, Tegtmeyer H, Desai P, Murthy S, et al. Tn, a carcinoma-associated antigen, reacts with anti-Tn of normal human sera. Cancer 1985; 55: 561-9, (Pubitemid 15156024)
44. Yao Z, Sakahara H, Zhang M, Kobayashi H, Nakada H, Yamashina I, Konishi J. Radioimmunoimaging of colon cancer xenografts with anti-Tn monoclonal antibody. Nucl Med Biol 1995; 22:199-203.
45. Zhang M, Yao Z, Sakahara H, Nakamoto Y, Sato N, Zhao S, et al. Effect of administration route and dose of streptavidin or biotin on the tumor uptake or radioactivity in intraperitoneal tumor with multistep targeting. Nucl Med Biol 1998; 25:101-5. (Pubitemid 28032161)
46. Avichezer D, Springer G, Schechter B, Arnon R. Immunoreactivities of polyclonal and monoclonal anti-T and anti-Tn antibodies with human carcinoma cells, grown in vitro and in a xenograft model. Int J Cancer 1997; 72: 119-27. (Pubitemid 27314648)
47. Babino A, Pritsch O, Oppezzo A, Du Pasquier R, Roseto A, Osinaga E, Alzari P. Molecular cloning of a monoclonal anti-tumor antibody specific for the Tn antigen and expression of an active single-chain Fv fragment. Hybridoma 1997; 16: 317-24. (Pubitemid 27410600)
48. Oppezzo P, Osinaga E, Tello D, Bay S, Cantacuzene D, Irigoin F, et al. Production and functional characterization of two mouse/human chimeric antibodies with specificity for the tumor-associated Tn antigen. Hybridoma 2000; 19: 229-39 . (Pubitemid 30622624)
49. Allen T. Ligand-targeted therapeutics in anticancer therapy. Nature Rev. Cancer 2002; 2: 750-63.
50. Brockhausen I. Biosynthesis and functions of O-glycans and regulation of mucin antigen expression in cancer. Biochem Soc Trans 1997, 25: 871-4. (Pubitemid 27416955)
51. Ogata S, Ho I, Chen A, Dubois D, Maklansky J, Singhal A, et al. Tumor-associates sialylated antigens are constitutively expressed in normal human colonic mucosa. Cancer Res 1995; 55: 1869-74.
52. Brockhausen T, Yang J, Dickinson N, Ogata S, Itzkowitz SH. Enzymatic basis for sialyl-Tn expression in human colon cancer cells. Glycoconj J 1998; 15: 595-603.
53. Vavasseur F, Yang JM, Dole K, Paulsen H, Brockhausen I. Synthesis of O-glycan core3: characterization of UDP-GlcNAc:GalNAc-R beta 3-N-acetyl-glucosaminyltrasnferase activity from colonic mucosal tissues and lack of the activity in human cancer cell lines. Glycobiology 1995; 5: 351-7.
54. Granovsky M, Bielfeldt T, Peters S, Paulsen H, Meldal M, Brockhausen J, Brockhausen I. UDPgalactose:glycoprotein-N-acetyl-D-galactosamine 3-b-D-galactosyltransferase activity synthesizing O-glycan core 1 is controlled by the amino acid sequence and glycosylation of glycopeptide substrates. Eur J Biochem 1994; 221: 1039-46.
55. Xu Y, Kimura N, Yoshida R, Lin H, Yoshinaga K. Immunohistochemical study of Muc1, Muc2 and human gastric mucin in breast carcinoma: relationship with prognostic factors. Oncol Rep 2001; 8: 1177-82.
56. Berois N, Varangot M, Sóñora C, Zarantonelli L, Pressa C, Laviña R, et al. Detection of bone marrow-disseminated breast cancer cells using a RT-PCR assay of MUC5B mRNA. Int J Cancer 2003; 103: 550-5.
57. De Bolos C, Guma M, Barranco C, Garrido M, Kim Y, Real FX. MUC6 expression in breast tissues and cultured cells: abnormal expression in tumors and regulation by steroid hormones. Int J Cancer 1998; 77: 193-9.
58. Freire T, Medeiros A, Reis C, Real FX, Osinaga E. Biochemical characterization of soluble Tn glycoproteins from malignant effusions of patients with carcinomas. Oncol Reports (in press).
59. Garraty G. Blood group antigens as tumor markers, parasitic/bacterial/ viral receptors, and their association with immunologically important proteins. Immunol Invest 1995, 24: 213-32.
60. Damian RT. Parasite immune evasion and exploitation: reflections and projections. Parasitology 1997; 115: S169-S175.
61. Persat F, Vincent C, Schmitt D, Mojon M. Inhibition of human peripheral blood mononuclear cell proliferative response by glycosphyngolipids from metacestode of Echinococcus multilocularis. Infect Immun 1996, 64: 3682-7.
62. Ellis LA, Reason AJ, Morris HR, Dell A, Iglesias R, Ubeira FM, Appleton JA. Glycans as targets for monoclonal antibodies that protect rats against Trichinella spiralis. Glycobiology 1994; 4: 585-92.
63. Cummings RD, Nyame D. Schistosome glycoconjugates. Biochim Biophys Acta 1999, 1455: 363-74.
64. Nyame K, Cummings RD, Damian RT. Schistosoma mansoni synthesizes glycoproteins containing terminal O-linked N-acetylglucosamine residues. J Biol Chem 1987; 262: 7990-5.
65. Alvarez Errico D, Medeiros A, Miguez M, Casaravilla C, Malgor R, Carmona C, Nieto A, Osinaga E. O-glycosylation in Echinococcus granulosus: identification and characterization of the carcinoma associated Tn antigen. Exp Parasitol 2001; 98: 100-9.
66. Freire T, Casaravilla C, Carmona C, Osinaga E. Mucin type O-glycosylation in Fasciola hepatica: characterization of carcinoma associated Tn and sialyl-Tn antigens and evaluation of UDP-GalNAc: polypeptide N- acetylgalactosaminyltransferase activity. Int J Parasitol 2003; 33: 47-56.
67. Casaravilla C, Freire T, Malgor R, Medeiros A, Osinaga E, Carmona C. Mucin-type O-glycosylation in helminth parasites from major taxonomic groups: evidence for widespread distribution of the Tn antigen (GalNAc-Ser/Thr) and identification of UDP-GalNAc: polypeptide N-acetylgalactosaminyltransferase activity. J Parasitol (in press).
68. Clapham PR, McKnight A. Cell surface receptors, virus entry and tropism of primate lentiviruses. J Gen Virol 2002; 83:1809-29.
69. Bernstein H, Tucker S, Hunter H, Schutzbach J, Compans R. Human immunodeficiency virus type 1 envelope glycoprotein is modified by O-linked oligosaccharides. J Virology 1994; 68: 463-8.
70. Hansen JES, Nielsen C, Arendrup M, Olofsson S, Mathiesen L, Nielsen J, Clausen H. Broadly neutralizing antibodies targeted to mucin-type carbohydrate epitopes of human immunodeficiency virus. J Virology 1991; 65: 6461-7.
71. Hansen, JE, Jansson B, Gram G, Clausen H, Nielsen J, Olofsson S. Sensitivity of HIV-1 to neutralization by antibodies against O-linked carbohydrate epitopes despite delection of O-glycosylation signals in the V3 loop. Arch Virology 1996; 141: 291-300.
72. Puri K, Gopalakrishnan B, Surolia A. Carbohydrate binding specificity of the Tn-antigen binding lectin from Vicia villosa seeds (VVLB4) FEBS Letters 1992; 312: 208-12.
73. Medeiros A, Bianchi S, Calvete J.J., Balter, H., Bay, S., Robles, A., et al. Biochemical and functional characterization of the Tn-specific lectin from Salvia sclarea seeds. Eur J Biochemistry 2000; 267: 1434-40.
74. Wu A, Wu J, Chen YY, Song SC, Kabat, E. Further characterization of the combining sites of Bandeirae (Griffonia) simplicifolia lectin-I, isolectin A4. Glycobiology 1999; 9:1161-70.
75. Reis C, Sorensen T, Mandel U, David L, Mirgorodskaya K, Roepstorff P, et al. Development and characterization of an antibody directed to an a-N-acetyl-galactosamine glycosylated MUC2 peptide. Glycoconj J 1998; 15: 51-62.
76. Nakada H, Inoue M, Numata Y, Tanaka N, Funakoshi I, Fukui S, et al. Epitopic structure of Tn glycophorin A for an anti-Tn antibody (MLS 128). Proc Natl Acad Sci (USA) 1993; 90: 2495-99.
77. Osinaga E, Bay S, Tello D, Babino, A, Pritsch O, Assemat K, et al. Analysis of the fine specificity of Tn-binding proteins using synthetic glycopeptide epitopes and a biosensor based on surface plasmon resonance spectroscopy. FEBS Letters 2000; 469: 24-8
78. Schlepper-Schäfer J, Springer G, Holl N, Kolb H, Kolb-Bachofen V. Macrophages from rat liver bind breast carcinoma cells and ingest tumor associated T and Tn antigens. Immunobiol Suppl. 1987; 3:180.
79. Iida S, Yamamoto K and Irimura T. Interaction of human macrophage C-type lectin with O-linked N-acetylgalactosamine residues on mucin glycopeptides. J Biol Chem 1999; 274: 10697-705.
80. Reddish M, Jackson L, Koganty R, Qiu D, Hong W, Longenecker BM. Specificities of anti-sialyl-Tn and anti-Tn monoclonal antibodies generated using novel clustered synthetic glycopeptide epitopes. Glicoconj J 1997; 14: 549-60.
81. Tanaka N, Nakada H, Inoue M, Yamashina I. Binding characteristics of an anti-Siaalpha2-6GalNAcalpha-Ser/Thr (sialylTn) monoclonal antibody (MLS132). Eur J Biochem 1999; 263:27-31.
82. Ogata S, Koganty R, Reddish M, Longenecker BM, Chen A, Perez C, Itzkowitz S. Different modes of sialyl-Tn expression during malignant transformation of human colonic mucosa Glicoconj J 1998; 15: 29-35.
83. Osinaga E, Tello D, Batthyany C, Bianchet M, Tavares G, Durán R, et al. Amino acid sequence and three-dimensional structure of the Tn-specific isolectin B4 from Vicia villosa. FEBS Letters 1997, 412:190-6.
84. Babino A, Tello D, Rojas A, Bay S, Osinaga E and Alzari P. The crystal structure of a plant lectin in complex with the Tn antigen. FEBS Lett 2003; 536: 106-110.
85. Singhal A, Fohn M, Hakomori S. Induction of N-acetylgalactosamine- O-serine/threonine (Tn) antigen-mediated cellular immune response for active immunotherapy in mice. Cancer Res 1991; 51: 1406-11.
86. Toyokuni T, Hakomori S, Singhal AK. Synthetic carbohydrate vaccines: synthesis and immunogenicity of Tn antigen conjugates. Bioorg Med Chem 1994;, 2: 1119-32.
87. Jensen T, Galli-Stampino L, Mouritsen S, Frische K, Peters S, Meldal M, Werdelin O. T cell recognition of Tn-glycosylated peptide antigens. Eur J Immunol 1996; 26: 1342-9.
88. Galli-Stampino L, Meinjohanns E, Frische K, Meldal M, Jensen T, Werdelin O, Mouritsen S. T-cell recognition of tumor-associated carbohydrates: the nature of the glycan moiety plays a decisive role in determining glycopeptide immunogenicity. Cancer Res 1997; 57: 3214-22.
89. Kuduk S, Schwarz J, Chen XT, Glunz P, Sames D, Ragupathi G, et al. Synthetic and immunological studies of clustered modes of mucin-related Tn and TF O-linked antigens: the preparation of a glycopeptide-based vaccines for clinical trials against prostate cancer. J Am Chem Soc 1998; 120: 12474-85.
90. Bay S, Lo-Man R, Osinaga E, Nakada H, Leclerc C, Cantacuzène D. Preparation of a multiple antigen glycopeptide (MAG) carrying the Tn antigen. A possible approach to a synthetic carbohydrate vaccine. J Peptide Res 1997; 49:620-5.
91. Lo-Man R, Bay S, Vichier-Guerre S, Deriaud E, Cantacuzene D, Leclerc C. A fully synthetic immunogen carrying a carcinoma-associated carbohydrate for active specific immunotherapy. Cancer Res 1999; 59: 1520-4.
92. Lo-Man R, Vichier-Guerre S, Bay S, Deriaud E, Cantacuzene D, Leclerc C. Anti-tumor immunity provided by a synthetic multiple antigenic glycopeptide displaying a tri-Tn glycotope. J Immunol 2001; 166: 2849-54.
93. Oliveira EC, Leite MS, Miranda JA, Andrade AL, Garcia SB, Luquetti AO, Moreira H. Chronic Trypanosoma cruzi infection associated with low incidence of 1,2-dimethylhydrazine-induced colon cancer in rats. Carcinogenesis 2001; 22: 737-40.
94. Shibao K, Izumi H, Nakayama Y, Ohta R, Nagata N, Nomoto M, et al. Expression of UDP-N-acetyl-a-D-galactosamine-polypeptide GalNAc N-acetylgalactosaminyl transferase-3 in relation to differentiation and prognosis in patients with colorectal carcinoma. Cancer 2002; 94:1939-46.
95. Dosaka-Akita H, Kinoshita I, Yamazaki K, Izumi H, Itoh T, Katoh H, et al. N-acetylgalactosaminyltransferase-3 is a potential new marker for non-small cell lung cancers. Br J Cancer 2002; 87: 751-5.
96. Schwientek T, Bennett E, Flores C, Thacker J, Hollmann M, Reis CA, et al. Functional Conservation of Subfamilies of Putative UDPN-acetylgalactosamine: Polypeptide N-Acetylgalactosaminyltransferases in Drosophila, Caenorhabditis elegans, and Mammals. One subfamily composed of l(2)35Aa is essential in Drosophila. J Biol Chem 2002; 277:22623-38.
97. Hirohashi S, Clausen H, Yamada T, Shimosato I, Hakomori S. Blood-group A crossreacting epitope defined by monoclonal antibodies NCC-LU-35 and -81 expressed in cancer of blood group O or B individuals. Its identification as Tn-antigen. Proc Natl Acad Sci (USA) 1985; 82: 7039-43.
98. Roxby D, Skinner J, Morley A, Weeks S, Burpee M. Expression of a Tn-like epitope by carcinoma cells. Br J Cancer 1987; 56: 734-7.
99. Longenecker M, Willans D, MacLean G, Selvaraj S, Suresh M, Noujaim A. Monoclonal antibodies and synthetic tumor-associated glycoconjugates in the study of the expression of Thompsen-Friedenreich-like and Tn-like antigens on human cancers. J Natl Cancer Inst 1987, 78:489-96.
100. Takahashi H, Metoki R, Hakomori S. Immunoglobulin G3 monoclonal antibody directed to Tn antigen (tumor-associated --N-acetylgalactosaminyl epitope) that does not cross-react with blood group A antigen. Cancer Res 1988; 48: 4361-7.
101. Springer G, Chandrasekaran EV, Desai P and Tegtmeyer H. Blood group Tn-active macromolecules from human carcinomas and erythrocytes: characterization of and specific reactivity with mono- and poly-clonal anti-Tn antibodies induced by various immunogens. Carbohydr Res 1988; 178:271-92.
102. Itzkowitz S, Yuan M, Montgomery C, Kjeldsen T, Takahashi H, Bigbee W, Kim Y. Expression of Tn, sialosyl-Tn, and T antigens in human colon cancer. Cancer Res 1989; 49:197-204.
103. Nakada H, Inoue M, Numata Y, Tanaka N, Funakoshi I, Fukui S, and Yamashina I. Cancer-associated glycoproteins defined by a monoclonal antibody, MLS128, recognizingnthe Tn antigen. Biochem Biophys Res Commun 1992; 187:217-24.
104. Huang J, Byrd J, Siddiki B, Yuan M, Lau E and Kim YS. Monoclonal antibodies against partially deglycosylated colon cancer mucin that recognize Tn antigen. Disease Markers 1992; 10: 81-94.
105. Thurnher M, Clausen H, Sharon N, Berger EG. Use of O-glycosylation- defective human lymphoid cell lines and flow cytometry to delineate the specificity of Moluccella laevis lectin and monoclonal 5F4 for the Tn-antigen (GalNAc alpha 1O-Ser/Thr). Immunol Lett 1993; 36: 239-43.
106. Osinaga E, Pancino G, Porchet N, Berois N, De Cremoux P, Mistro D, et al. Analysis of a heterogeneous group of breast carcinoma associated glycoproteins bearing Tn determinant. Breast Cancer Res Treat 1994; 32: 139-52.
107. Tollefsen S, Kornfeld R. The B4 lectin from Vicia villosa seeds interacts with N-acetylgalactosamine residues alpha-linked to serine or threonine residues in cell surface glycoproteins. J Biol Chem 1983; 258: 5172-6.
108. Piller V, Piller F, Cartron JP. Isolation and characterization of an N-acetylgalactosamine specific lectin from Salvia sclarea seeds. J Biol Chem 1986; 261: 14069-75.
109. Duk M, Mitra D, Lisowska E, Kabat E, Sharon N, Lis H. Immunochemical studies on the combining site of the A+N blood type specific Moluccella laevis lectin. Carbohydr Res 1992; 236: 245-58.