Binding characteristics of an anti-Siaα2-6GalNAcα-Ser/Thr (sialyl Tn) monoclonal antibody (MLS 132)

European Journal of Biochemistry

Volumn 263, Issue 1, 1999, Pages 27-31

  Tanaka, Nobuhiro ^a   Nakada, Hiroshi ^a   Inoue, Mizue ^a   Yamashina, Ikuo ^a  

^a KYOTO SANGYO UNIVERSITY   (Japan)

Author keywords

Carbohydrate antigen; Epitope; Monoclonal antibody; Mucins; O linked oligosaccharides

Indexed keywords

EPITOPE; MONOCLONAL ANTIBODY; MUCIN; SIALOPROTEIN;

AMINO ACID SEQUENCE; ANTIGEN BINDING; ARTICLE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; SHEEP; SIALYLATION; SUBMANDIBULAR GLAND;

EID: 0033167940     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00401.x     Document Type: Article

References (17)

1. 1. Hakomori, S.-I. (1985) Aberrant glycosylation in cancer cell membranes as focused on glycolipids. Cancer Res. 45, 2405-2414.
2. 2. Feizi, T. (1985) Demonstration by monoclonal antibodies that carbohydrate structures of glycoproteins are onco-developmental antigens. Nature 314, 53-57.
3. 3. Larkin, M., Knapp, W., Stoll, M.S., Mehmet, H. & Feizi, T. (1991) Monoclonal antibodies VIB-E3, IB5 and HB9 to the leucocyte/ epithelial antigen CD24 resemble BA-1 in recognizing sialic acid-dependent epitope (s). Evidence that VIB-E3 recognizes NeuAc alpha 2-6GalNAc and NeuAc alpha 2-6Gal sequences. Clin. Exp. Immunol. 85, 536-541.
4. 4. Stein, R., Chen, S., Grossman, W. & Goldenberg, D.M. (1989) Human lung carcinoma monoclonal antibody specific for the Thomsen-Friedenreich antigen. Cancer Res. 49, 32-37.
5. 5. Hirohashi, S., Clausen, H., Yamada, T., Shimosato, Y. & Hakomori, S.-I. (1985) Blood group A crossreacting epitope defined by monoclonal antibodies NCC-LU-35 and -81 expressed in cancer of Blood group O or B individuals: Its identification as Tn antigen. Proc. Natl Acad. Sci. USA 82, 7039-7043.
6. 6. Takahashi, H., Metoki, R. & Hakomori, S.-I. (1988) Immunoglobulin G3 monoclonal antibody directed to Tn antigen (tumor associated α-N-Acetylgalactosaminyl epitope) that does not cross-react with blood group A antigen. Cancer Res. 48, 4361-4367.
7. 7. Kurosaka, A., Kitagawa, H., Fukui, S., Numata, Y., Nakada, H., Funakoshi, I., Kawasaki, T., Ogawa, T., Iijima, H. & Yamashina, I. (1988) A monoclonal antibody that recognizes a cluster of a disaccharide, NeuAcα2-6Ga1NAc, in mucin-type glycoproteins. J. Biol. Chem. 263, 8724-8726.
8. 8. Kurosaka, A., Fukui, S., Kitagawa, H., Nakada, H., Numata, Y., Funakoshi, I., Kawasaki, T. & Yamashina, I. (1987) Mucin-carbohydrate directed monoclonal antibody. FEBS Lett. 215, 137-139.
9. 9. Kjeldsen, T., Clausen, H., Hirohashi, S., Ogawa, T., Iijima, H. & Hakomori, S.-I. (1988) Preparation and characterization of monoclonal antibodies directed to the tumor associated O-linked sialosylα2-6N-acetylgalactosaminyl (sialosyl-Tn) epitope. Cancer Res. 48, 2214-2220.
10. 10. Fukui, S., Numata, Y., Kurosaka, A., Kitagawa, H., Nakada, H., Funakoshi, I., Kawasaki, T., Takahashi, Y., Hayashi, K. & Yamashina, I. (1988) Production of monoclonal antibodies directed against carbohydrate moieties of cell surface glycoproteins. Jpn. J. Cancer Res. 79, 1119-1129.
11. 11. Numata, Y., Nakada, H., Fukui, S., Kitagawa, H., Ozaki, K., Inoue, M., Kawasaki, T., Funakoshi, I. & Yamashina, I. (1990) A monoclonal antibody directed to Tn antigen. Biochem. Biophys. Res. Commun. 170, 981-985.
12. 12. Nakada, H., Numata, Y., Inoue, M., Tanaka, N., Kitagawa, H., Funakoshi, I., Fukui, S. & Yamashina, I. (1991) Elucidation of an essential structure recognized by an anti-GalNAcα-Ser (Thr) monoclonal antibody (MLS 128). J. Biol. Chem. 266, 12402-12405.
13. 13. Nakada, H., Inoue, M., Numata, Y., Tanaka, N., Funakoshi, I., Fukui, S., Mellors, A. & Yamashina, I. (1993) Epitopic structure of Tn glycophorin A for an anti-Tn antibody (MLS 128). Proc. Natl Acad. Sci. USA 90, 2495-2499.
14. 14. Tettamanti, G. & Pigman, W. (1968) Isolation and characterization of glycoproteins from ovine submaxillary mucin: purification and characterization of bovine and ovine submaxillary mucins. Arch. Biochem. Biophys. 124, 45-50.
15. 15. Schneider, C., Newman, R.A., Sutherland, D.R., Assev, V. & Greves, M.F. (1982) A one-step purification of membrane proteins using a high efficiency immunomatrix. J. Biol. Chem. 243, 616-626.
16. 16. Hara, S., Yamaguchi, M., Takemori, Y., Fukuhata, K., Ogura, H. & Nakamura, M. (1989) Determination of mono-O-acetylneuraminic acids in human and rat sera by fluorometric high-performance liquid chromatography. Anal. Biochem. 179, 162-166.
17. 17. Hill, H.D. Jr, Schwyzer, M., Steinman, H. & Hill, R.L. (1977) Ovine submaxillary mucin: primary structure and peptide substrates of UDP-N-acetylgalactosamine: mucin transferase. J. Biol. Chem. 252, 3799-3804.