Production and purification of self-assembling peptides in Ralstonia eutropha

Protein Expression and Purification

Volumn 46, Issue 2, 2006, Pages 179-188

  Reed, David C ^a   Barnard, Gavin C ^a   Anderson, Errik B ^a   Klein, Linden T ^a   Gerngross, Tillman U ^a  

^a DARTMOUTH COLLEGE   (United States)

Author keywords

Carbohydrate binding modules; Cellulose binding domains; Enzymatic cleavage; Fermentation; High cell density; Multimeric fusion proteins; Multimerization; Recombinant peptides; Sapeptides; Self assembling peptides; Self assembly; Tissue engineering

Indexed keywords

CUPRIAVIDUS NECATOR;

EID: 33645416315     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.08.023     Document Type: Article

References (59)

1. T.C. Holmes Extensive neurite outgrowth and active synapse formation on self-assembling peptide scaffolds Proc. Natl. Acad. Sci. USA 97 2000 6728 6733
2. J. Kisiday Self-assembling peptide hydrogel fosters chondrocyte extracellular matrix production and cell division: implications for cartilage tissue repair Proc. Natl. Acad. Sci. USA 99 2002 9996 10001
3. C.E. Semino, J.R. Merok, G.G. Crane, G. Panagiotakos, and S. Zhang Functional differentiation of hepatocyte-like spheroid structures from putative liver progenitor cells in three-dimensional peptide scaffolds Differentiation 71 2003 262 270
4. S. Zhang, T. Holmes, C. Lockshin, and A. Rich Spontaneous assembly of a self-complementary oligopeptide to form a stable macroscopic membrane Proc. Natl. Acad. Sci. USA 90 1993 3334 3338
5. S. Zhang Self-complementary oligopeptide matrices support mammalian cell attachment Biomaterials 16 1995 1385 1393
6. M.R. Caplan, P.N. Moore, S. Zhang, R.D. Kamm, and D.A. Lauffenburger Self-assembly of a beta-sheet protein governed by relief of electrostatic repulsion relative to van der Waals attraction Biomacromolecules 1 2000 627 631
7. T.C. Holmes Novel peptide-based biomaterial scaffolds for tissue engineering Trends Biotechnol. 20 2002 16 21
8. M.R. Caplan, E.M. Schwartzfarb, S. Zhang, R.D. Kamm, and D.A. Lauffenburger Control of self-assembling oligopeptide matrix formation through systematic variation of amino acid sequence Biomaterials 23 2002 219 227
9. S. Srinivasan, G. Barnard, and T.U. Gerngross A novel high-cell-density protein expression system based on Ralstonia eutropha Appl. Environ. Microbiol. 68 2002 5925 5932
10. S. Srinivasan, G. Barnard, and T.U. Gerngross Production of recombinant proteins using multiple-copy gene integration in high-cell-density fermentations of Ralstonia eutropha Biotechnol. Bioeng. 84 2003 114 120
11. G. Barnard, G.E. Henderson, S. Srinivasan, and T.U. Gerngross High level recombinant protein expression in Ralstonia eutropha using T7 RNA polymerase based amplification Protein Express. Purif. 38 2004 264 271
12. G.C. Barnard, J. McCool, D.W. Wood, T.U. Gerngross, An integrated recombinant protein expression and purification platform based on Ralstonia eutropha, Appl. Environ. Microbiol. (2005) in press.
13. K. Itakura Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin Science 198 1977 1056 1063
14. D.V. Goeddel Expression in Escherichia coli of chemically synthesized genes for human insulin Proc. Natl. Acad. Sci. USA 76 1979 106 110
15. R. Wetzel Production of biologically active N alpha-desacetylthymosin alpha 1 in Escherichia coli through expression of a chemically synthesized gene Biochemistry 19 1980 6096 6104
16. J. Shine, I. Fettes, N.C. Lan, J.L. Roberts, and J.D. Baxter Expression of cloned beta-endorphin gene sequences by Escherichia coli Nature 285 1980 456 463
17. S. Sumi, F. Nagawa, T. Hayashi, H. Amagase, and M. Suzuki Overproduction of a gastrointestinal hormone, secretin, in Escherichia colicells and its chemical characterization Gene 29 1984 125 134
18. J. Nilsson, S. Stahl, J. Lundeberg, M. Uhlen, and P.A. Nygren Affinity fusion strategies for detection, purification, and immobilization of recombinant proteins Protein Express. Purif. 11 1997 1 16
19. M.T. Doel The expression in E. coli of synthetic repeating polymeric genes coding for poly(L-aspartyl-L-phenylalanine) Nucleic Acids Res. 8 1980 4575 4592
20. S.H. Shen Multiple joined genes prevent product degradation in Escherichia coli Proc. Natl. Acad. Sci. USA 81 1984 4627 4631
21. T. Kempe Multiple-copy genes: production and modification of monomeric peptides from large multimeric fusion proteins Gene 39 1985 239 245
22. M.F. Schultz, G. Buell, E. Schmid, R. Movva, and G. Selzer Increased expression in Escherichia coli of a synthetic gene encoding human somatomedin C after gene duplication and fusion J. Bacteriol. 169 1987 5385 5392
23. M. Lennick, J.R. Haynes, and S.H. Shen High-level expression of alpha-human atrial natriuretic peptide from multiple joined genes in Escherichia coli Gene 61 1987 103 112
24. T. Murata, S. Horinouchi, and T. Beppu Production of poly(l-aspartyl-l- phenylalanine) in Escherichia coli J. Biotechnol. 28 1993 301 312
25. A. Kuliopulos, and C.T. Walsh Production, purification, and cleavage of tandem repeats of recombinant peptides J. Am. Chem. Soc. 116 1994 4599 4607
26. J. Raingeaud Production, analysis and bioactivity of recombinant vasoactive intestinal peptide analogs Biochimie 78 1996 14 25
27. H. Ishikawa, and H. Tamaoki Production of human calcitonin in Escherichia coli from multimeric fusion protein J. Ferm. Bioeng. 82 1996 140 144
28. J.H. Lee, I. Minn, C.B. Park, and S.C. Kim Acidic peptide-mediated expression of the antimicrobial peptide buforin II as tandem repeats in Escherichia coli Protein Express. Purif. 12 1998 53 60
29. C.J. Park, J.H. Lee, S.S. Hong, H.S. Lee, and S.C. Kim High-level expression of the angiotensin-converting-enzyme-inhibiting peptide, YG-1, as tandem multimers in Escherichia coli Appl. Microbiol. Biotechnol. 50 1998 71 76
30. P. Jonasson Integrated bioprocess for production of human proinsulin C-peptide via heat release of an intracellular heptameric fusion protein J. Biotechnol. 76 2000 215 226
31. S.J. Lee A novel technique for the effective production of short peptide analogs from concatameric short peptide multimers Mol. Cell 10 2000 236 240
32. S. Dilsen Fed-batch production of recombinant human calcitonin precursor fusion protein using Staphylococcus carnosus as an expression-secretion system Appl. Microbiol. Biotechnol. 54 2000 361 369
33. J.H. Lee, M.S. Kim, J.H. Cho, and S.C. Kim Enhanced expression of tandem multimers of the antimicrobial peptide buforin II in Escherichia coli by the DEAD-box protein and trxB mutant Appl. Microbiol. Biotechnol. 58 2002 790 796
34. K.S. Oh, D.K. Na, M.H. Kweon, and H.C. Sung Expression and purification of the anticomplementary peptide Sh-CRIT-ed1 (formerly Sh-TOR-ed1) as a tetramultimer in Escherichia coli Protein Express. Purif. 27 2003 202 209
35. C. Li, M.L. Ng, Y. Zhu, B. Ho, and J.L. Ding Tandem repeats of Sushi3 peptide with enhanced LPS-binding and -neutralizing activities Protein Eng. 16 2003 629 635
36. P. Tomme Characterization and affinity applications of cellulose-binding domains J. Chrom. B 715 1998 283 296
37. I. Levy, and O. Shoseyov Cellulose-binding domains: biotechnological applications Biotechnol. Adv. 20 2002 191 213
38. G.R. Drapeau, Y. Boily, and J. Houmard Purification and properties of an extracellular protease of Staphylococcus aureus J. Biol. Chem. 247 1972 6720 6726
39. S.B. Sorensen, T.L. Sorensen, and K. Breddam Fragmentation of proteins by S. aureus strain V8 protease. Ammonium bicarbonate strongly inhibits the enzyme but does not improve the selectivity for glutamic acid FEBS Lett. 294 1991 195 197
40. G. Carrard, A. Koivula, H. Soderlund, and P. Beguin Cellulose-binding domains promote hydrolysis of different sites on crystalline cellulose Proc. Natl. Acad. Sci. USA 97 2000 10342 10347
41. J.B. Owolabi, P. Beguin, D.G. Kilburn, R.C. Miller Jr., and A.J. Warren Expression in Eschericia coli of the Cellulomonas fimi Structural Gene for Endoglucanase B Appl. Environ. Microbiol. 54 1988 518 523
42. Y. Berdichevsky Matrix-assisted refolding of single-chain Fv-cellulose binding domain fusion proteins Protein Express. Purif. 17 1999 249 259
43. J.B. Coutinho, B. Moser, D.G. Kilburn, R.A. Warren, and R.C. Miller Jr. Nucleotide sequence of the endoglucanase C gene (cenC) of Cellulomonas fimi, its high-level expression in Escherichia coli, and characterization of its products Mol. Microbiol. 5 1991 1221 1233
44. K.P. Fuchs, V.V. Zverlov, G.A. Velikodvorskaya, F. Lottspeich, and W.H. Schwartz Lic16A of Clostridium thermocellum, a non-cellulosomal, highly complex endo-beta-1,3-glucanase bound to the outer cell surface Microbiology 149 2003 1021 1031
45. H. Simpson, and F. Barras Functional analysis of the carbohydrate-binding domains of Erwinia chrysanthemi Cel5 (Endoglucanase Z) and an Escherichia coli putative chitinase J. Bacteriol. 181 1999 4611 4616
46. A.C. Fernandes Homologous xylanases from Clostridium thermocellum: evidence for bi-functional activity, synergism between xylanase catalytic modules and the presence of xylan-binding domains in enzyme complexes Biochem. J. 342 1999 105 110
47. O. Grepinet, M.C. Chebrou, and P. Beguin Nucleotide sequence and deletion analysis of the xylanase gene (xynZ) of Clostridium thermocellum J. Bacteriol. 170 1988 4582 4588
48. M. Kavoosi Inexpensive one-step purification of polypeptides expressed in Escherichia coli as fusions with the family 9 carbohydrate-binding module of xylanase 10A from T. maritima J. Chrom. B 807 2004 87 94
49. J.S. Park, H.C. Park, T.L. Huh, and Y.H. Lee Production of poly-beta-hydroxybutyrate by Alcigenes eutrophus transformants harbouring cloned phbCAB genes Biotechnol. Lett. 17 1995 735 740
50. J. Sambrook, and D. Russel Molecular Cloning: A Laboratory Manual 2001 Cold Springs Harbor Laboratory Press Cold Springs Harbor, NY
51. G.M. York, B.H. Junker, J. Stubbe, and A.J. Sinskey Accumulation of the PhaP phasin of Ralstonia eutropha is dependent on production of polyhydroxybutyrate in cells J. Bacteriol. 183 2001 4217 4226
52. R. Simon, U. Priefer, and A. Pfihler A broad host-range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram-negative bacteria Biotechnology 1 1983 784 791
53. V. de Lorenzo, M. Herrero, U. Jakubzik, and K.N. Timmis Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in Gram-negative eubacteria J. Bacteriol. 172 1990 6568 6572
54. J. Quandt, and M.F. Hynes Versatile suicide vectors which allow direct selection for gene replacement in Gram-negative bacteria Gene 127 1993 15 21
55. M.F. Alexeyev The pKnock series of broad-host-range mobilizable suicide vectors for gene knockout and targeted DNA insertions into the chromosome of Gram-negative bacteria BioTechniques 26 1999 824 826
56. R. Skeoch, Synpep Co., personal communication, 2005.
57. J.E. Bailey, and D.F. Ollis Biochemical Engineering Fundamentals 1986 McGraw-Hill New York
58. S. Zhang Fabrication of novel biomaterials through molecular self-assembly Nat. Biotechnol. 21 2003 1171 1178
59. P.W. Latham Therapeutic peptides revisited Nat. Biotechnol. 17 1999 755 757