High level recombinant protein expression in Ralstonia eutropha using T7 RNA polymerase based amplification

Protein Expression and Purification

Volumn 38, Issue 2, 2004, Pages 264-271

  Barnard, Gavin C ^a   Henderson, Grant E ^a   Srinivasan, Sriram ^a   Gerngross, Tillman U ^a  

^a DARTMOUTH COLLEGE   (United States)

Author keywords

Fermentation; High cell density; Organophosphate hydrolase; Parathion hydrolase; Phosphotriesterase; Recombinant protein; T7 RNA polymerase

Indexed keywords

BACTERIOPHAGE T7 RNA POLYMERASE; DNA DIRECTED RNA POLYMERASE; HYBRID PROTEIN; PHOSPHATASE; VIRUS PROTEIN;

ARTICLE; ENZYME ACTIVATION; ENZYMOLOGY; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION REGULATION; GENE VECTOR; GENETICS; METABOLISM; MOLECULAR CLONING; PROMOTER REGION; TIME; WAUTERSIA EUTROPHA;

CLONING, MOLECULAR; CUPRIAVIDUS NECATOR; DNA-DIRECTED RNA POLYMERASES; ENZYME ACTIVATION; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENETIC VECTORS; PHOSPHORIC MONOESTER HYDROLASES; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; TIME FACTORS; VIRAL PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; RALSTONIA; TRANSPOSONS; UNIDENTIFIED CLONING VECTOR; WAUTERSIA; WAUTERSIA EUTROPHA;

EID: 8844262654     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.09.001     Document Type: Article

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