Slow aggregation of lysozyme in alkaline pH monitored in real time employing the fluorescence anisotropy of covalently labelled dansyl probe

FEBS Letters

Volumn 580, Issue 8, 2006, Pages 2097-2101

  Homchaudhuri, Lopamudra ^a   Kumar, Satish ^a   Swaminathan, Rajaram ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY GUWAHATI   (India)

Author keywords

Arginine; Dansyl; Fluorescence anisotropy; Lysozyme aggregation

Indexed keywords

ARGININE; DANSYL CHLORIDE; EGG WHITE; GUANIDINE; LYSOZYME;

ALKALINITY; ANISOTROPY; ARTICLE; CHEMICAL LABELING; FLUORESCENCE ANALYSIS; PRIORITY JOURNAL; PROTEIN AGGREGATION; STEADY STATE;

ANIMALS; ANISOTROPY; DANSYL COMPOUNDS; FLUORESCENCE POLARIZATION; HYDROGEN-ION CONCENTRATION; MURAMIDASE; PROTEIN STRUCTURE, QUATERNARY; TIME FACTORS;

EID: 33645211553     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2006.03.012     Document Type: Article

References (18)

1. D. Thirumalai, D.K. Klimov, and R.I. Dima Emerging ideas on the molecular basis of protein and peptide aggregation Curr. Opin. Struct. Biol. 13 2003 146 159
2. D.M. Walsh, I. Klyubin, J.V. Fadeeva, W.K. Cullen, R. Anwyl, M.S. Wolfe, M.J. Rowan, and D.J. Selkoe Naturally secreted oligomers of amyloid beta protein potently inhibit hippocampal long term potentiation in vivo Nature 416 2002 535 539
3. M. Bucciantini, E. Giannoni, F. Chiti, F. Baroni, L. Formigli, J. Zurdo, N. Taddei, G. Ramponi, C.M. Dobson, and M. Stefani Inherent toxicity of aggregates implies a common mechanism for misfolding diseases Nature 416 2002 507 511
4. M.P. Lambert, A.K. Barlow, B.A. Chromy, C. Edwards, R. Freed, M. Liosatos, T.E. Morgan, I. Rozovsky, B. Trommer, and K.L. Viola Diffusible, nonfibrillar ligands from Abeta 1-42 are potent central nervous system neurotoxins Proc. Natl. Acad. Sci. USA 95 1998 6448 6453
5. B. Caughey, and P.T. Lansbury Jr. Protofibrils, pores, fibrils and neurodegeneration: Separating the responsible protein aggregates from the innocent bystanders Annu. Rev. Neurosci. 26 2003 267 298
6. R. Swaminathan, N. Periasamy, J.B. Udgaonkar, and G. Krishnamoorthy Molten globule like conformation of barstar: a study by fluorescence dynamics J. Phys. Chem. 98 1994 9270 9278
7. J.R. Lakowicz Principles of Fluorescence Spectroscopy 1999 Kluwer Academic/Plenum Press New York
8. A.J. Sophianopoulos, and K.E. van Holde Evidence for dimerization of lysozyme in alkaline solution J. Biol. Chem. 236 1961 PC82 PC83
9. A.J. Sophianopoulos, and K.E. van Holde Physical studies of muramidase (lysozyme): pH dependent dimerization J. Biol. Chem. 239 1964 2516 2524
10. L.N. Arnaudov, and R. de Vries Thermally induced fibrillar aggregation of hen egg white lysozyme Biophys. J. 88 2005 515 526
11. M.R.H. Krebs, D.K. Wilkins, E.W. Chung, M.C. Pitkeathly, A.K. Chamberlain, J. Zurdo, C.V. Robinson, and C.M. Dobson Formation and seeding of amyloid fibrils from wild-type hen lysozyme and a peptide fragment from the beta domain J. Mol. Biol. 300 2000 541 549
12. K. Vos, A. van Hoek, and A.J. Visser Application of a reference convoloution method to tryptophan fluorescence in proteins. A refined description of rotational dynamics Eur. J. Biochem. 165 1987 55 63
13. R.C.K. Reddy, H. Lilie, R. Rudolph, and C. Lange l-Arginine increases the solubility of unfolded species of hen egg white lysozyme Protein Sci. 14 2005 929 935
14. V. Levi, and F.L.G. Flecha Labeling of proteins with fluorescent probes. Photophysical characterization of dansylated bovine serum albumin Biochem. Mol. Biol. Educ. 31 2003 333 336
15. R.F. Chen Dansyl labeled proteins: determination of extinction coefficient and number of bound residues with radioactive dansyl chloride Anal. Biochem. 25 1968 412 416
16. D. Allsop, L. Swanson, S. Moore, Y. Davies, A. York, O.M.A. El-Agnaf, and I. Soutar Fluorescence anisotropy: a method for early detection of Alzheimer beta-peptide(Aβ) aggregation Biochem. Biophys. Res. Commun. 285 2001 58 63
17. E. Feinstein, G. Deikus, E. Rusinova, E.L. Rachofsky, J.B.A. Ross, and W.R. Laws Constrained analysis of fluorescence anisotropy decay: application to experimental protein dynamics Biophys. J. 84 2003 599 611
18. B.M. Baynes, D.I.C. Wang, and B.L. Trout Role of arginine in stabilisation of proteins against aggregation Biochemistry 44 2005 4919 4925