Fluorescence anisotropy: A method for early detection of Alzheimer β-peptide (Aβ) aggregation

Biochemical and Biophysical Research Communications

Volumn 285, Issue 1, 2001, Pages 58-63

  Allsop, David ^a   Swanson, Linda ^b   Moore, Susan ^a   Davies, Yvonne ^a   York, Amber ^a   El Agnaf, Omar M A ^a   Soutar, Ian ^b  

^a LANCASTER UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

Alzheimer's disease; Amyloid; A ; Fluorescein; Time resolved fluorescence anisotropy

Indexed keywords

AMYLOID BETA PROTEIN; PEPTIDE; THIOFLAVINE;

ALZHEIMER DISEASE; ANISOTROPY; ARTICLE; COMPLEX FORMATION; CONCENTRATION RESPONSE; EARLY DIAGNOSIS; FLUORESCENCE ANALYSIS; MEASUREMENT; PATHOGENESIS; PRIORITY JOURNAL; PROTEIN AGGREGATION; SCREENING;

EID: 0034789110     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2001.5123     Document Type: Article

References (24)

1. Amyloid deposition as the central event in the aetiology of Alzheimer's disease
2. Translating cell biology into therapeutic advances in Alzheimer's disease
3. The Aβ peptide of Alzheimer's disease directly produces hydrogen peroxide through metal ion reduction
4. α-synuclein implicated in Parkinson's disease catalyses the formation of hydrogen peroxide in vitro
5. Morphology and toxicity of Aβ-(1-42) dimer derived from neuritic and vascular amyloid deposits of Alzheimer's disease
6. Amyloid β-protein fibrillogenesis; structure and biological function of protofibrillar intermediates
7. Protofibrillar intermediates of amyloid β-protein induce acute electrophysiological changes and progressive neurotoxicity in cortical neurons
8. The influence of the central region containing residues 19-25 on the aggregation properties and secondary structure of Alzheimer's β-amyloid peptide
9. The N-terminal region of non-Aβ component of Alzheimer's disease amyloid is responsible for its tendency to assume β-sheet and aggregate to form fibrils
10. Prolines and amyloidogenicity in fragments of the Alzheimer's peptide Aβ/A4
11. Aggregation and metal-binding properties of mutant forms of the amyloid Aβ peptide of Alzheimer's disease
12. Detection of single amyloid β-protein aggregates in the cerebrospinal fluid of Alzheimer's patients by fluorescence correlation spectroscopy
13. Sensitive detection of β-amyloid aggregation by means of time-resolved fluorescence anisotropy
14. Modulation of β-amyloid production and fibrillisation
15. Improved solid-phase syntheses of amyloid proteins associated with neurodegenerative diseases
16. Fluorometric determination of amyloid fibrils in vitro using the fluorescent dye, thioflavin T
17. An FT-IR study of the β-amyloid conformation: Standardization of aggregation grade
18. Models of amyloid seeding in Alzheimer's disease and scrapie: Mechanistic truths and physiological consequences of time-dependent solubility of amyloid proteins
19. Soluble amyloid Aβ-(1-40) exists as a stable dimer at low concentrations
20. Amyloid β-protein fibrillogenesis: Detection of a protofibrillar intermediate
21. Fluorescence anisotropy studies of polyelectrolyte mobility and interpolyelectrolyte complexation in aqueous solution
22. Fluorescence studies of the dynamic behaviour of poly(dimethylacrylamide) and its complex with poly(methacrylic acid) in dilute solution
23. An ultrastructural study of amyloid intermediates in Aβ 1-42 fibrillogenesis
24. Kinetic theory of fibrillogenesis of amyloid β-protein