Antibiotic resistance and mechanisms implicated in clinical enterococci in a Tunisian Hospital

Journal of Chemotherapy

Volumn 18, Issue 1, 2006, Pages 20-26

  Klibi, N ^a,b   Gharbi, S ^a   Masmoudi, A ^c   Ben Slama, K ^a   Poeta, P ^b   Zarazaga, M ^b   Fendri, C ^c   Boudabous, A ^a   Torres, Carmen ^b  

^a FACULTÉ DES SCIENCES DE TUNIS   (Tunisia)

^b UNIVERSITY OF LA RIOJA   (Spain)

^c LA RABTA HOSPITAL   (Tunisia)

Author keywords

Antibiotic resistance; Enterococci; Resistance genes; Tunisia

Indexed keywords

AMINOGLYCOSIDE ANTIBIOTIC AGENT; AMOXICILLIN; ANTIBIOTIC AGENT; CHLORAMPHENICOL; COTRIMOXAZOLE; ERYTHROMYCIN; GENTAMICIN; IMIPENEM; KANAMYCIN; PENICILLIN G; PRISTINAMYCIN; RIFAMPICIN; SPIRAMYCIN; STREPTOMYCIN; TEICOPLANIN; TETRACYCLINE; VANCOMYCIN;

AAC(6') APH(2'') GENE; ANT(6) IA GENE; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; APH(3') IIIA GENE; ARTICLE; BACTERIAL GENE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CAT(A) GENE; ENTEROCOCCUS; ENTEROCOCCUS AVIUM; ENTEROCOCCUS CASSELIFLAVUS; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; ENTEROCOCCUS GALLINARUM; ENTEROCOCCUS HIRAE; ERM(A) GENE; ERM(B) GENE; ERM(C) GENE; HOSPITAL INFECTION; MEFA E GENE; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; POLYMERASE CHAIN REACTION; PULSED FIELD GEL ELECTROPHORESIS; TET(L) GENE; TET(M) GENE; TUNISIA;

EID: 33645095990     PISSN: 1120009X     EISSN: None     Source Type: Journal    
DOI: 10.1179/joc.2006.18.1.20     Document Type: Article

References (39)

1. Low D, Keller N, Barth A, Jones, N. Clinical prevalence, antimicrobial susceptibility, and geographic resistance patterns of enterococci: Results from the SENTRY antimicrobial surveillance program, 1997-1999. Clin Infect Dis 2001; 32 (suppl 2): S133-S144.
2. Murray BE. Diversity among multidrug-resistant enterococci. Emerg Infect Dis 1998; 4 (1): 37-47.
3. Udo E, Al-Sweih N, Phillips OA, Chugh TD. Species prevalence and antibacterial resistance of enterococci isolated in Kuwait hospitals. J Med Microbiol 2003; 52 (2): 163-168.
4. Casetta A, Buu Hoy A, Cespedes G, Horaud T. Diversity of structures carrying the high-level gentamicin resistance gene (aac6-aph2) in Enterococcus faecalis strains isolated in France. Antimicrob Agents Chemother 1998; 42 (11): 2889-2892.
5. Daikos GL, Bamias G, Kattamis C, et al. Structures, locations, and transfer frequencies of genetic elements conferring high-level gentamicin resistance in Enterococcus faecalis isolates in Greece. Antimicrob Agents Chemother 2003; 47 (12): 3950-3953.
6. Pournaras S, Tsakris A, Palepou M, et al. Pheromone responses and high-level aminoglycoside resistance of conjugative plasmids of Enterococcus faecalis from Greece. J Antimicrob Chemother 2003; 46 (6): 1013.
7. Del Campo R, Tenorio C, Rubio C, Castillo J, Torres C, Gómez-Lus R. Aminoglycoside-modifying enzymes in high-level streptomycin and gentamicin resistant Enterococcus spp in Spain. Int J Antimicrob Agents 2000; 15 (3): 221-226.
8. Portillo A, Ruiz-Larrea F, Zarazaga M, Alonso A, Martinez JL, Torres C. Macrolide resistance genes in Enterococcus spp. Antimicrob Agents Chemother 2000; 44 (4): 967-971.
9. Mutnick A, Biedenbach D, Jones R. Geographic variations and trends in antimicrobial resistance among Enterococcus faecalis and Enterococcus faecium in the SENTRY antimicrobial surveillance program (1997-2000). Diagn Microbiol Infect Dis 2003; 46 (1): 63-68.
10. Kobayashi N, Alam M, Nishimoto Y, Urasawa S, Uehara N, Watanabe N. Distribution of aminoglycoside resistance genes in recent clinical isolates of Enterococcus faecalis, Enterococcus faecium and Enterococcus avium. Epidemiol Infect 2001; 126 (2): 197-204.
11. Chopra I, Roberts M. Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance. Microbiol Mol Biol Rev 2001; 65 (2): 232-260.
12. Roberts MC. Epidemiology of tetracycline-resistant determinants. Trends Microbiol 1994; 2 (10): 353-357.
13. Aarestrup FM, Agerso Y, Gerner-Smidt P, Madsen M, Jensen L. Comparison of antimicrobial resistance phenotypes and resistance genes in Enterococcus faecalis and Enterococcus faecium from humans in the community, broilers, and pigs in Danmark. Diagn Microbiol Infect Dis 2000; 37 (2): 127-137.
14. Del Campo R, Ruiz-Garbajosa P, Sanchez-Moreno MP, et al. Antimicrobial resistance in recent fecal enterococci from healthy volunteers and food handlers in Spain: genes and phenotypes. Microb Drug Res 2003; 9 (1): 47-60.
15. Cetinkaya Y, Falk P, Mayhall C. Vancomycin-resistant enterococci. Clin Microbiol Rev 2000; 13 (4): 686-707.
16. Depardieu F, Bonora MG, Reynolds PE, Courvalin P. The vanG glycopeptide resistance operon from Enterococcus faecalis revisited. Mol Microbiol 2003; 50 (3): 931-948.
17. Abassi MS, Achour W, Ben Hassan A. Characteristics of Enterococcus strains isolated from neutropenic patients at the National Bone-Marrow Transplantation Center of Tunis. Bull Soc Pathol Exot 2004; 97: 91-94.
18. Kechrid A, Ben Rejeb S, Gargouri J, Fendri C, Ben Hassan E, Ben Boujnah A. Group D streptococci and enterococci: identification, sensitivity to antibiotics and a study of the high level resistance to aminosides (Charles Nicolle Hospital in Tunis). Med Trop 1991; 51 (2): 177-180.
19. Kechrid A, Barguellil F, Slim L, Amor A, Horaud T. Biochemical identification of species of the Enterococcus genus isolated from a hospital environment in Tunis, antibiotic sensitivity. Tunis Med 1996; 74 (12): 545-548.
20. Dutka-Malen S, Evers S, Courvalin P. Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J Clin Microbiol 1995; 33 (5): 24-27.
21. th InterScience Conference of Antimicrobial Agents and Chemotheraphy, abstr. 1576, San Francisco, 1999.
22. Miele A, Bandera M, Goldstein BP. Use of primers selective for vancomycin resistance genes to determine van genotype in enterococci and to study gene organization in vanA isolates. Antimicrob Agents Chemother 1995; 39 (8): 1772-1778.
23. Members of the SFM Antibiogram Committee. Comité de l'antibiogramme de la Société Française de Microbiologie. Report 2003. Int J Antimicrob Agents 2003; 21 (4): 364-391.
24. Turabelidze D, Kotetishvili M, Kreger A, Morris J, Sulakvelidze A. Improved pulsed -field gel electrophoresis for typing vancomycin-resistant enterococci. J Clin Microbiol 2000; 38 (11): 4242-4245.
25. Tenover FC, Arbeit RD, Goering RV, et al. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 1995; 33 (9): 2233-2239.
26. Dicuonzo G, Gherardi G, Lorino G, et al. Antibiotic resistance and genotypic characterization by PFGE of clinical and environmental isolates of enterococci. FEMS Microbiol Lett 2001; 201 (2): 205-211.
27. Zouain M, Araj G. Antimicrobial resistance of enterococci in Lebanon. Int J Antimicrob Agents 2000; 17 (3): 209-213.
28. Fontana R, Ligozzi M, Pittaluga F, Satta G. Intrinsic penicillin resistance in enterococci. Microb Drug Resist 1996; 2 (2): 209-213.
29. Schmitz F-J, Verhoef J, Fluit A, and the SENTRY Participants Group. Prevalence of aminoglycoside resistance in 20 European university hospitals participating in the European SENTRY antimicrobial surveillance programme. Eur J Clin Microbiol Infect. Dis 1999; 18 (6): 414-421.
30. Schouten M, Voss A, Hoogkamp-Korstanje J, and the European VRE study group. Antimicrobial susceptibility patterns of enterococci causing infections in Europe. Antimicrob Agents Chemother 1999; 43 (10): 2542-2546.
31. Leclercq R, Derlot E, Duval J, Courvalin P. Plasmid-mediated resistance to vancomycin and teicoplanin in Enterococcus faecium. New Engl J Med 1988; 319 (3): 157-161.
32. Uttley AHC, Collins CH, Naidoo J, George RC. Vancomycin-resistant enterococci. Lancet 1988; 1 (8575-6): 57-58.
33. Vandamme P, Vercauteren E, Lammens C, et al. Survey of enterococcal susceptibility patterns in Belgium. J Clin Microbiol 1996; 34 (10): 2572-2576.
34. Wu J, Hsueh P, Lu J, Teng L, Luh K. Antimicrobial susceptibilities of clinical isolates of VRE in Taiwan. Clin Invest Med 1999; 22: 256-264.
35. von Gottberg A, van Nierop W, Duse A, et al. Epidemiology of glycopeptide-resistant enterococci colonizing high-risk patients in hospitals in Johannesburg, Republic of South Africa. J Clin Microbiol 2000; 38 (2): 905-909.
36. Schmitz F, Sadurski R, Kray A, et al. Prevalence of macrolide-resistance genes in Staphylococcus aureus and Enterococcus faecium isolates from 24 European university hospitals. J Antimicrob Chemother 2000; 45 (6): 891-894.
37. Donabedian S, Thai L, Hershberger E, et al. Molecular characterization of gentamicin-resistant enterococci in the United States: Evidence of spread from animals to humans through food. J Clin Microbiol 2003; 41 (3): 1109-1113.
38. Van de Klundert J, Vliegenthart J. PCR detection of genes coding for aminoglycoside-modifying enzymes. In D.H. Persing (eds), Diagnostic Molecular Microbiology. ASM. 1993.
39. Sutcliffe J, Grebe T, Tait-Kamradt A, Wondrack L. Detection of erythromycin-resistant determinants by PCR. Antimicrob Agents Chemother 1996; 40 (11): 2562-2566.