Structures, Locations, and Transfer Frequencies of Genetic Elements Conferring High-Level Gentamicin Resistance in Enterococcus faecalis Isolates in Greece

Antimicrobial Agents and Chemotherapy

Volumn 47, Issue 12, 2003, Pages 3950-3953

  Daikos, George L ^a,e   Bamias, George ^a   Kattamis, Christos ^a   Zervos, Marcus J ^b   Chow, Joseph W ^c   Christakis, George ^d   Petrikkos, George ^a   Triantafyllopoulou, Penelope ^a   Alexandrou, Helen ^a   Syriopoulou, Vassiliki ^a  

^a UNIVERSITY OF ATHENS   (Greece)

^b METAXA CANCER HOSPITAL   (Greece)

^c WILLIAM BEAUMONT HOSPITAL   (United States)

^d WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^e LAIKO GENERAL HOSPITAL   (Greece)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; GENOMIC DNA; GENTAMICIN; PLASMID DNA; RESTRICTION ENDONUCLEASE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENETICS; BACTERIAL STRAIN; BACTERIUM ISOLATE; CONTROLLED STUDY; CORRELATION ANALYSIS; ENTEROCOCCUS FAECALIS; GREECE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; TRANSPOSON;

ACETYLTRANSFERASES; ANTI-BACTERIAL AGENTS; CHROMOSOMES, BACTERIAL; CONJUGATION, GENETIC; DNA TRANSPOSABLE ELEMENTS; DNA, BACTERIAL; DRUG RESISTANCE, BACTERIAL; ELECTROPHORESIS, AGAR GEL; ENTEROCOCCUS FAECALIS; GENTAMICINS; GRAM-POSITIVE BACTERIAL INFECTIONS; GREECE; HUMANS; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PLASMIDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 10744231058     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/AAC.47.12.3950-3953.2003     Document Type: Article

References (23)

1. Byrne, M. E., M. T. Gillespie, and R. A. Skurray. 1990. Molecular analysis of a gentamicin resistance transposonlike element on plasmids isolated from North American Staphylococcus aureus strains. Antimicrob. Agents Chemother. 34:2106-2113.
2. Byrne, M. E., D. A. Rouch, and R. A. Skurray. 1989. Nucleotide sequence analysis of IS256 from the Staphylococcus aureus gentamicin-tobramycin-kanamycin-resistance transposon Tn4001. Gene 81:361-367.
3. Casetta, A., A. B. Hoi, G. D. Cespedes, and T. Horaud. 1998. Diversity of structures carrying the high-level gentamicin resistance gene (aac6-aph2) in Enterococcus faecalis strains isolated in France. Antimicrob. Agents Chemother. 42:2889-2892.
4. Clewell, D. B., P. K. Tomich, C. Gawron-Burke, A. E. Franke, Y. Yagi, and F. Y. An. 1982. Mapping of Streptococcus faecalis plasmids pAD1 and pAD2 and studies relating to transposition of Tn917. J. Bacteriol. 152:1220-1230.
5. Ferreti, J. J., K. S. Gilmore, and P. Courvalin. 1986. Nucleotide sequence analysis of the gene specifying the bifunctional 6′ -aminoglycoside acetyltransferase 2″-aminoglycoside phosphotranferase enzyme in Streptococcus faecalis and identification and cloning of gene regions specifying the two activities. J. Bacteriol. 167:631-638.
6. Forbes, B. A., and D. R. Schaberg. 1983. Transfer of resistance plasmids from Staphylococcus epidermidis to Staphylococcus aureus: evidence for conjugative exchange of resistance. J. Bacteriol. 153:627-634.
7. Hodel-Christian, S. L., and B. E. Murray. 1991. Characterization of the gentamicin resistance transposon Tn5281 from Enterococcus faecalis and comparison to staphylococcal transposons Tn4001 and Tn4031. Antimicrob. Agents Chemother. 35:1147-1152.
8. Hodel-Christian, S. L., and B. E. Murray. 1992. Comparison of the gentamicin resistance transposon Tn5281 with regions encoding gentamicin resistance in Enterococcus faecalis isolates from diverse geographic locations. Antimicrob. Agents Chemother. 36:2259-2264.
9. Hoffmann, S. A., and R. C. Moellering. 1987. The Enterococcus: "putting the bug in our ears." Ann. Intern. Med. 106:757-761.
10. Lyon, B. R., M. T. Gillespie, M. E. Byrne, J. W. May, and R. A. Skurray. 1987. Plasmid-mediated resistance to gentamicin in Staphylococcus aureus: the involvement of a transposon. J. Med. Microbiol. 23:101-110.
11. Murray, B. E. 1990. The life and times of the Enterococcus. Clin. Microbiol. Rev. 3:46-65.
12. Murray, B. E., K. V. Singh, J. D. Heath, B. R. Sharma, and G. M. Weinstock. 1990. Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases with infrequent recognition sites. J. Clin. Microbiol. 28:2059-2063.
13. National Committee for Clinical Laboratory Standards. 1999. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, 5th ed. Approved standard M7-A5. National Committee for Clinical Laboratory Standards, Wayne, Pa.
14. Patterson, J. E., and M. J. Zervos. 1990. High-level gentamicin resistance in Enterococcus: microbiology, genetic basis, and epidemiology. Rev. Infect. Dis. 12:644-652.
15. Rice L. B., and L. L. Carias. 1998. Transfer of Tn5385, a composite, multiresistance chromosomal element from Enterococcus faecalis. J. Bacteriol. 180:714-721.
16. Rice, L. B., L. L. Carias, and S. H. Marshall. 1995. Tn5384, a composite enterococcal mobile element conferring resistance to erythromycin and gentamicin whose ends are directly repeated copies of IS256. Antimicrob. Agents Chemother. 39:1147-1153.
17. Rice, L. B., and A. S. Thorisdottir. 1994. The prevalence of sequences homologous to IS256 in clinical enterococcal isolates. Plasmid 32:344-349.
18. Rouch, D. A., M. E. Byrne, Y. C. Kong, and R. A. Skurray. 1987. The aacA-aphD gentamicin and kanamycin resistance determinant of Tn4001 from Staphylococcus aureus: expression and nucleotide sequence analysis. J. Gen. Microbiol. 133:3039-3052.
19. Straut, M., G. de Cespedes, F. Delbos, and T. Horaud. 1997. Molecular typing of Enterococcus faecalis strains resistant to high levels of gentamicin isolates in Romania. J. Antimicrob. Chemother. 39:483-491.
20. Tenover, F. C., R. D. Arbeit, R. V. Goering, P. A. Mickelsen, B. E. Murray, D. H. Persing, and B. Swaminathan. 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microbiol. 33:2233-2239.
21. Thal, L. A., J. W. Chow, D. B. Clewell, and M. J. Zervos. 1994. Tn924, a chromosome-borne transposon encoding high-level gentamicin resistance in Enterococcus faecalis. Antimicrob. Agents Chemother. 38:1152-1156.
22. Thai, L. A., J. W. Chow, J. E. Patterson, M. B. Perri, S. Donabedian, D. B. Clewell, and M. J. Zervos. 1993. Molecular characterization of highly gentamicin-resistant Enterococcusfaecalis isolates lacking high-level streptomycin resistance. Antimicrob. Agents Chemother. 37:134-137.
23. Wirth, R., F. Y. An, and D. B. Clewell. 1986. Highly efficient protoplast transformation system for Streptococcusfaecalis and a new Escherichia coli-S. faecalis shuttle vector. J. Bacteriol. 165:831-836.