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Volumn 351, Issue 1, 2006, Pages 36-43

Methacarn fixation - Effects of tissue processing and storage conditions on detection of mRNAs and proteins in paraffin-embedded tissues

Author keywords

Expression analysis; Methacarn; Molecular integrity; Paraffin embedded tissue

Indexed keywords

DEHYDRATION; GENE EXPRESSION; HISTOLOGY; PARAFFINS; PROTEINS;

EID: 33645032798     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2006.01.032     Document Type: Article
Times cited : (21)

References (25)
  • 2
    • 0031879859 scopus 로고    scopus 로고
    • Identification of expressed genes by laser-mediated manipulation of single cells
    • K. Schütze, and G. Lahr Identification of expressed genes by laser-mediated manipulation of single cells Nat. Biotechol. 16 1998 737 742
    • (1998) Nat. Biotechol. , vol.16 , pp. 737-742
    • Schütze, K.1    Lahr, G.2
  • 3
    • 0034975536 scopus 로고    scopus 로고
    • A new method for histological microdissection utilizing an ultrasonically oscillating needle: Demonstrated by differential mRNA expression in human lung carcinoma tissue
    • M. Harsch, K. Bendrat, G. Hofmeier, D. Branscheid, and A. Niendorf A new method for histological microdissection utilizing an ultrasonically oscillating needle: demonstrated by differential mRNA expression in human lung carcinoma tissue Am. J. Pathol. 158 2001 1985 1990
    • (2001) Am. J. Pathol. , vol.158 , pp. 1985-1990
    • Harsch, M.1    Bendrat, K.2    Hofmeier, G.3    Branscheid, D.4    Niendorf, A.5
  • 4
    • 0014713238 scopus 로고
    • Methacarn (Methanol-Carnoy) fixation. Practical and theoretical considerations
    • H. Puchtler, F.S. Waldrop, S.N. Meloan, M.S. Terry, and H.M. Conner Methacarn (Methanol-Carnoy) fixation. Practical and theoretical considerations Histochemie 21 1970 97 116
    • (1970) Histochemie , vol.21 , pp. 97-116
    • Puchtler, H.1    Waldrop, F.S.2    Meloan, S.N.3    Terry, M.S.4    Conner, H.M.5
  • 5
    • 0034049097 scopus 로고    scopus 로고
    • Methacarn fixation: A novel tool for analysis of gene expressions in paraffin-embedded tissue specimens
    • M. Shibutani, C. Uneyama, K. Miyazaki, K. Toyoda, and M. Hirose Methacarn fixation: a novel tool for analysis of gene expressions in paraffin-embedded tissue specimens Lab. Invest. 80 2000 199 208
    • (2000) Lab. Invest. , vol.80 , pp. 199-208
    • Shibutani, M.1    Uneyama, C.2    Miyazaki, K.3    Toyoda, K.4    Hirose, M.5
  • 6
    • 0001349119 scopus 로고    scopus 로고
    • Methacarn, a fixation tool for multipurpose gene expression analysis from paraffin-embedded tissue materials
    • C. Uneyama, M. Shibutani, K. Nakagawa, N. Masutomi, and M. Hirose Methacarn, a fixation tool for multipurpose gene expression analysis from paraffin-embedded tissue materials Curr. Topics Biochem. Res. 3 2000 237 242
    • (2000) Curr. Topics Biochem. Res. , vol.3 , pp. 237-242
    • Uneyama, C.1    Shibutani, M.2    Nakagawa, K.3    Masutomi, N.4    Hirose, M.5
  • 7
    • 0036407162 scopus 로고    scopus 로고
    • Methacarn: A fixation tool for multipurpose genetic analysis from paraffin-embedded tissues
    • P.M. Conn Academic Press London
    • M. Shibutani, and C. Uneyama Methacarn: a fixation tool for multipurpose genetic analysis from paraffin-embedded tissues P.M. Conn Laser Capture Microscopy and Microdissection, Methods Enzymol vol. 356 2002 Academic Press London 114 125
    • (2002) Laser Capture Microscopy and Microdissection, Methods Enzymol , vol.356 , pp. 114-125
    • Shibutani, M.1    Uneyama, C.2
  • 8
    • 0036735310 scopus 로고    scopus 로고
    • Methacarn fixation for genomic DNA analysis in microdissected, paraffin-embedded tissue specimens
    • C. Uneyama, M. Shibutani, N. Masutomi, H. Takagi, and M. Hirose Methacarn fixation for genomic DNA analysis in microdissected, paraffin-embedded tissue specimens J. Histochem. Cytochem. 50 2002 1237 1245
    • (2002) J. Histochem. Cytochem. , vol.50 , pp. 1237-1245
    • Uneyama, C.1    Shibutani, M.2    Masutomi, N.3    Takagi, H.4    Hirose, M.5
  • 10
    • 33746678640 scopus 로고    scopus 로고
    • Methacarn fixation for genomic DNA analysis in microdissected cells
    • G.I. Murray, S. Curran (Eds.), Laser Capture Microdissection and its Applications, Humana Press, Totowa
    • M. Shibutani, C. Uneyama, Methacarn fixation for genomic DNA analysis in microdissected cells, in: G.I. Murray, S. Curran (Eds.), Laser Capture Microdissection and its Applications, Humana Press, Totowa, Methods Mol. Biol. 293 (2004) 11-25.
    • (2004) Methods Mol. Biol. , vol.293 , pp. 11-25
    • Shibutani, M.1    Uneyama, C.2
  • 12
    • 0031663606 scopus 로고    scopus 로고
    • Regulation of rat hepatic cytochrome P450 expression by sterol biosynthesis inhibition: Inhibitors of squalene synthase are potent inducers of CYP2B expression in primary cultured rat hepatocytes and rat liver
    • T.A. Kocarek, J.M. Kraniak, and A.B. Reddy Regulation of rat hepatic cytochrome P450 expression by sterol biosynthesis inhibition: inhibitors of squalene synthase are potent inducers of CYP2B expression in primary cultured rat hepatocytes and rat liver Mol. Pharmacol. 54 1998 474 484
    • (1998) Mol. Pharmacol. , vol.54 , pp. 474-484
    • Kocarek, T.A.1    Kraniak, J.M.2    Reddy, A.B.3
  • 13
    • 0034668118 scopus 로고    scopus 로고
    • Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: Comparison of endpoint and real-time methods
    • T.D. Schmittgen, B.A. Zakrajsek, A.G. Mills, V. Gorn, M.J. Singer, and M.W. Reed Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods Anal. Biochem. 285 2000 194 204
    • (2000) Anal. Biochem. , vol.285 , pp. 194-204
    • Schmittgen, T.D.1    Zakrajsek, B.A.2    Mills, A.G.3    Gorn, V.4    Singer, M.J.5    Reed, M.W.6
  • 14
    • 0036898005 scopus 로고    scopus 로고
    • Effect of fixatives and tissue processing on the content and integrity of nucleic acids
    • M. Srinivasan, D. Sedmak, and S. Jewell Effect of fixatives and tissue processing on the content and integrity of nucleic acids Am. J. Pathol. 161 2002 1961 1971
    • (2002) Am. J. Pathol. , vol.161 , pp. 1961-1971
    • Srinivasan, M.1    Sedmak, D.2    Jewell, S.3
  • 16
    • 12244292719 scopus 로고    scopus 로고
    • HOPE-a new fixing technique enables preservation and extraction of high molecular weight DNA and RNA of >20 kb from paraffin-embedded tissues. Hepes-glutamic acid buffer mediated organic solvent protection effect
    • K.H. Wiedorn, J. Olert, R.A. Stacy, T. Goldmann, H. Kühl, J. Matthus, E. Vollmer, and A. Bosse HOPE-a new fixing technique enables preservation and extraction of high molecular weight DNA and RNA of >20 kb from paraffin-embedded tissues. Hepes-glutamic acid buffer mediated organic solvent protection effect Pathol. Res. Pract. 198 2002 735 740
    • (2002) Pathol. Res. Pract. , vol.198 , pp. 735-740
    • Wiedorn, K.H.1    Olert, J.2    Stacy, R.A.3    Goldmann, T.4    Kühl, H.5    Matthus, J.6    Vollmer, E.7    Bosse, A.8
  • 19
    • 0026535112 scopus 로고
    • The AMeX method: A multipurpose tissue-processing and paraffin-embedding method. Extraction of protein and application to immunoblotting
    • Y. Sato, K. Mukai, S. Furuya, T. Kameya, and S. Hirohashi The AMeX method: a multipurpose tissue-processing and paraffin-embedding method. Extraction of protein and application to immunoblotting Am. J. Pathol. 140 1992 775 779
    • (1992) Am. J. Pathol. , vol.140 , pp. 775-779
    • Sato, Y.1    Mukai, K.2    Furuya, S.3    Kameya, T.4    Hirohashi, S.5
  • 21
    • 0142106515 scopus 로고    scopus 로고
    • A tissue fixative that protects macromolecules (DNA, RNA, and protein) and histomorphology in clinical samples
    • V. Vincek, M. Nassiri, M. Nadji, and A.R. Morales A tissue fixative that protects macromolecules (DNA, RNA, and protein) and histomorphology in clinical samples Lab. Invest. 83 2003 1427 1435
    • (2003) Lab. Invest. , vol.83 , pp. 1427-1435
    • Vincek, V.1    Nassiri, M.2    Nadji, M.3    Morales, A.R.4
  • 22
  • 23
    • 0020422396 scopus 로고
    • Tissue preparation for the demonstration of surface antigens by immunoperoxidase techniques
    • M.A. Judd, and K.J. Britten Tissue preparation for the demonstration of surface antigens by immunoperoxidase techniques Histochem. J. 14 1982 747 753
    • (1982) Histochem. J. , vol.14 , pp. 747-753
    • Judd, M.A.1    Britten, K.J.2
  • 24
    • 0019518689 scopus 로고
    • Inhibition of endogenous tissue alkaline phosphatase with the use of alkaline phosphatase conjugates in immunohistochemistry
    • B.A. Ponder, and M.M. Wilkinson Inhibition of endogenous tissue alkaline phosphatase with the use of alkaline phosphatase conjugates in immunohistochemistry J. Histochem. Cytochem. 29 1981 981 984
    • (1981) J. Histochem. Cytochem. , vol.29 , pp. 981-984
    • Ponder, B.A.1    Wilkinson, M.M.2
  • 25
    • 3543141103 scopus 로고    scopus 로고
    • Long-term preservation of antigenicity on tissue microarrays
    • K.A. DiVito, L.A. Charette, D.L. Rimm, and R.L. Camp Long-term preservation of antigenicity on tissue microarrays Lab. Invest. 84 2004 1071 1078
    • (2004) Lab. Invest. , vol.84 , pp. 1071-1078
    • Divito, K.A.1    Charette, L.A.2    Rimm, D.L.3    Camp, R.L.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.