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33644889669
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note
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The increase in conductance in the presence of HCI is due to the higher permeability of protons through gramicidin channels compared to potassium ions.
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37
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33644908505
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note
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Over the course of the reaction, we observed an intermediate current state of 17.9pA, which presumably arose from the conversion of 1 to an intermediate of the reaction (e.g., the carbamic acid) that converts more slowly to product 2. The observed current at 17.9 pA might arise from a combination of indistinguishable conductance states generated from this intermediate of the reaction and/or asymmetric gramicidin channels formed from this intermediate and 2. Indistinguishable conductance states between symmetric and asymmetric gramicidin A channels have been previously reported in ref. [1d].
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40
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33644887473
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note
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For both of the reactions we describe in this work, the final products are the same regardless of whether the synthetic transformations occur while the peptides are in the membrane or in the aqueous phase. Although the order in which the reagents are added to the reaction vessels will not change the final products of the reactions, it is possible that the reaction rates are influenced by the local environment of the peptide. Therefore the observed kinetics of the reactions might depend on the order in which the reagents are added to the bilayer setup.
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