Enteropathogenic Escherichia coli inhibits butyrate uptake in Caco-2 cells by altering the apical membrane MCT1 level

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 290, Issue 1, 2006, Pages

  Borthakur, Alip ^a   Gill, Ravinder K ^a   Hodges, Kim ^a   Ramaswamy, Krishnamurthy ^a   Hecht, Gail ^a   Dudeja, Pradeep K ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

Author keywords

Diarrhea; Ion transport; Monocarboxylate transporter 1; Short chain fatty acids

Indexed keywords

ADENOSINE TRIPHOSPHATASE; BACTERIAL PROTEIN; BUTYRIC ACID; CARRIER PROTEINS AND BINDING PROTEINS; MONOCARBOXYLATE TRANSPORTER; MONOCARBOXYLATE TRANSPORTER 1; STRUCTURAL PROTEIN; UNCLASSIFIED DRUG; BUTYRIC ACID DERIVATIVE; COTRANSPORTER; ESCHERICHIA COLI PROTEIN; MONOCARBOXYLATE TRANSPORT PROTEIN 1;

APICAL MEMBRANE; ARTICLE; BACTERIAL GENE; BACTERIAL MUTATION; BACTERIAL STRAIN; BACTERIUM MUTANT; CELL STRAIN CACO 2; CONTROLLED STUDY; ENTEROPATHOGENIC ESCHERICHIA COLI; GENE FUNCTION; HUMAN; HUMAN CELL; INFANTILE DIARRHEA; INTESTINE EPITHELIUM CELL; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN SYNTHESIS INHIBITION; WILD TYPE; CELL MEMBRANE; CELL POLARITY; ESCHERICHIA COLI; METABOLISM; PHYSIOLOGY; TIME; TRANSPORT AT THE CELLULAR LEVEL;

BIOLOGICAL TRANSPORT; BUTYRATES; CACO-2 CELLS; CELL MEMBRANE; CELL POLARITY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HUMANS; MONOCARBOXYLIC ACID TRANSPORTERS; SYMPORTERS; TIME FACTORS;

EID: 33644802277     PISSN: 01931857     EISSN: 15221547     Source Type: Journal    
DOI: 10.1152/ajpgi.00302.2005     Document Type: Article

References (32)

1. + exchanger 3 is in large complexes in the center of the apical surface of proximal tubule-derived OK cells. Am J Physiol Cell Physiol 283: C927-C940, 2002.
2. Alrefai WA, Tyagi S, Gill R, Saksena S, Hadjiagapiou C, Mansour F, Ramaswamy K, and Dudeja PK. Regulation of butyrate uptake in Caco2 cells by phorbol 12-myristate 13-acetate. Am J Physiol Gastrointest Liver Physiol 286: G197-G203, 2004.
3. Binder HJ and Mehta P. Short chain fatty acids stimulate Na and Cl absorption in vitro in the rat distal colon. Gastroenterology 96: 989-996, 1989.
4. Bradford M. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254, 1976.
5. Buyse M, Sitaraman SV, Liu X, Bado A, and Merlin D. Luminal leptin enhances CD147/MCT1 mediated uptake of butyrate in the human intestinal cell line Caco2-BBE. J Biol Chem 277: 28182-28190, 2002.
6. Cook SI and Sellin JH. Review article: short chain fatty acids in health and disease. Pharmacol Ther 12: 499-507, 1998.
7. Donnenberg MS, Yu J, and Kaper JB. A second chromosomal gene necessary for intimate attachment of enteropathogenic E. coli to epithelial cells. J Bacteriol 175: 4670-4680, 1993.
8. Elliott SJ, Krejany EO, Mellies JL, Robins-Browne RM, Sasakawa C, and Kaper JB. EspG, a novel type III system-secreted protein from enteropathogenic E. coli with similarities to VirA of Shigella flexneri. Infect Immun 69: 4027-4033, 2001.
9. Gauthier A, Puente JL, and Finlay BB. Secretion of the enteropathogenic E. coli type III secretion system requires components of the type III apparatus for assembly and localization. Infect Immun 71: 3310-3319, 2003.
10. - exchange activity in Caco-2 cells (Abstract). Gastroenterology 126: A-95, 2004.
11. Hadjiagapiou C, Schmidt L, Dudeja PK, Lyden TJ, and Ramaswamy K. Mechanism(s) of butyrate uptake in Caco2 cells: Role of monocarboxylate transporter 1. Am J Physiol Gastrointest Liver Physiol 279: G775-G780, 2000.
12. Harig JM, Ng EK, Dudeja PK, Brasitus TA, and Ramaswamy K. Transport of n-butyrate into human colonic luminal membrane vesicles. Am J Physiol Gastrointest Liver Physiol 271: G415-G422, 1996.
13. - exchange activity in Caco2 cells (Abstract). Gastroenterology 124: A482, 2003.
14. + exchange isoform activities by enteropathogenic E. coli in human intestinal epithelial cells. Am J Physiol Gastrointest Liver Physiol 287: G370-G378, 2004.
15. - secretion. Am J Physiol Gastrointest Liver Physiol 276: G781-G788, 1999.
16. Inan MS, Rasoulpour RJ, Yin L, Habbard AK, Rosenberg DW, and Giardena C. The luminal short chain fatty acid butyrate modulate NFkappaB activity in a human colonic epithelial cell line. Gastroenterology 118: 724-734, 2000.
17. Jarvis KG, Giron JA, Jerse AE, McDaniel TK, Donnenberg MS, and Kaper JB. Enteropathogenic E. coli contains a putative type III secretion system necessary for the export of proteins involved in attaching and effacing lesion formation. Proc Natl Acad Sci USA 92: 7996-8000, 1995.
18. Kenny B and Jepson M. Targeting of an EPEC effector protein to host mitochondria. Cell Microbiol 2: 579-590, 2000.
19. Kiela PR, Hines ER, Collins JF, and Ghisan FK. Regulation of the rat NHE3 gene promoter by sodium butyrate. Am J Physiol Gastrointest Liver Physiol 281: G947-G956, 2001.
20. Lai LC, Wainwright LA, Stone KD, and Donnenberg MS. A third secreted protein that is encoded by the enteropathogenic E. coli pathogenicity island is required for transduction of signals and for attaching and effacing activities in host cells. Infect Immun 65: 2211-2217, 1997.
21. Matsuzawa T, Kuwae A, Yoshida S, sasakawa C, and Abe A. Enteropathogenic E. coli activates the RhoA signalling pathway via the stimulation of GEF-H1. EMBO J 23: 3570-3582, 2004.
22. McDaniel TK and Kaper JB. A cloned pathogenicity island from enteropathogenic E. coli confers the attaching and effacing phenotype on E coli k-12. Mol Microbiol 23: 399-407, 1997.
23. McNamara BP, Koutsouris A, O'conell CB, Nougayrede JP, Donnenberg MS, and Hecht G. Translocated EspF protein from enteropathogenic E. coli disrupts host intestinal barrier function. J Clin Invest 107:621- 629, 2001.
24. Montrose MH and Kere J. Anion absorption in the intestine: anion transporters, short chain fatty acids, and role of the DRA gene product. Curr Top Membr Transp 50: 301-928, 2001.
25. Musch MW, Bookstein C, Xie Y, Sellin JH, and Chang EB. Short chain fatty acids increase intestinal Na absorption by induction of NHE3 in rat colon and human intestinal C2/bbe cells. Am J Physiol Gastrointest Liver Physiol 280: G687-G693, 2001.
26. Muza-Moons MM, Schneeberger EE, and Hecht GA. Enteropathogenic E. coli infection leads to appearance of aberrant tight junction strands in the lateral membrane of intestinal epithelial cells. Cell Microbiol 6: 783-793, 2004.
27. Resta-Lenert S, Trung F, Barrett KE, and Eckmann L. Inhibition of epithelial chloride secretion by butyrate: role of reduced adenylyl cyclase expression and activity. Am J Physiol Cell Physiol 281: C1837-C1849, 2001.
28. Ritzhaupt A, Wood IS, Ellis A, Hosie KB, and Shirazi-Beechey SP. Identification and characterization of a monocarboxylate transporter. J Physiol 513: 719-732, 1998.
29. Savkovic SV, Koutsouris A, and Hecht A. Activation of NF-kappaB in intestinal epithelial cells by enteropathogenic E. coli. Am J Physiol Cell Physiol 273: C1160-C1167, 1997.
30. Tomson FL, Viswanathan VK, Kanack KJ, Kanteti RP, Straub KV, Menet M, Kaper JB, and Hecht G. Enteropathogenic E. coli EspG disrupts microtubules and in conjunction with Orf3 enhances perturbation of the tight junction barrier. Mol Microbiol 56: 447-464, 2005.
31. Tu X, Nisan I, Yona C, Hanski E, and Rosenshine I. EspH, a new cytoskeleton-modulating effector of enterohaemorrhagic and enteropathogenic E. coli. Mol Microbiol 47: 595-606, 2003.
32. Vallance BA and Finlay BB. Exploitation of host cells by enteropathogenic E. coli. Proc Natl Acad Sci USA 97: 8799-8806, 2000.