Differential regulation of Na+/H+ exchange isoform activities by enteropathogenic E. coli in human intestinal epithelial cells

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 287, Issue 2 50-2, 2004, Pages

  Hecht, Gail ^b   Hodges, Kim ^b   Gill, Ravinder K ^b   Kear, Fely ^b   Tyagi, Sangeeta ^b   Malakooti, Jaleh ^b   Ramaswamy, Krishnamurthy ^b   Dudeja, Pradeep K ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

^b NONE

Author keywords

Diarrhea; Enteric pathogens; Ion transport; Na+ absorption

Indexed keywords

5 (N ETHYL N ISOPROPYL)AMILORIDE; AMILORIDE; I KAPPA B ALPHA; SODIUM PROTON EXCHANGE PROTEIN 1; SODIUM PROTON EXCHANGE PROTEIN 2; SODIUM PROTON EXCHANGE PROTEIN 3; SODIUM PROTON EXCHANGE PROTEIN INHIBITOR; UNCLASSIFIED DRUG;

ARTICLE; CELL SPECIFICITY; CONTROLLED STUDY; DIARRHEA; DISEASE ASSOCIATION; DISEASE COURSE; EARLY DIAGNOSIS; ESCHERICHIA COLI; GENE DISRUPTION; GENE MUTATION; HUMAN; HUMAN CELL; INFLAMMATION; INTESTINE ABSORPTION; INTESTINE EPITHELIUM CELL; MICROVILLUS; PATHOGENESIS; PATHOPHYSIOLOGY; PRIORITY JOURNAL; PROTON SODIUM EXCHANGE; STATISTICAL SIGNIFICANCE; TIME;

AMILORIDE; CACO-2 CELLS; CELL MEMBRANE; ENTERITIS; ESCHERICHIA COLI INFECTIONS; ESCHERICHIA COLI PROTEINS; HUMANS; INTESTINAL ABSORPTION; INTESTINAL MUCOSA; INTRACELLULAR MEMBRANES; OSMOTIC PRESSURE; PROTEIN ISOFORMS; SODIUM; SODIUM-HYDROGEN ANTIPORTER; TIME FACTORS;

EID: 3242658157     PISSN: 01931857     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpgi.00432.2003     Document Type: Article

References (43)

1. + exchanger isoform NHE3 by PKC-α in Caco-2 cells. Am J Physiol Cell Physiol 281: C1551-C1558, 2001.
2. Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254, 1976.
3. + exchange near the base of mouse colonic crypts. Am J Physiol Cell Physiol 283: C358-C372, 2002.
4. Collington GK, Booth IW, Donnenberg MS, Kaper JB, and Knutton S. Enteropathogenic Escherichia coli virulence genes encoding secreted signalling proteins are essential for modulation of Caco-2 cell electrolyte transport. Infect Immun 66: 6049-6053, 1998.
5. Collington GK, Booth IW, and Knutton S. Rapid modulation of electrolyte transport in Caco-2 cell monolayers by enteropathogenic Escherichia coli (EPEC) infection. Gut 42: 200-207, 1998.
6. Daniell SJ, Takahashi N, Wilson R, Friedberg D, Rosenshine I, Booy FP, Shaw RK, Knutton S, Frankel G, and Aizawa S. The filamentous type III secretion translocon of enteropathogenic Escherichia coli. Cell Microbiol 3: 865-871, 2001.
7. Donnenberg MS, Yu J, and Kaper JB. A second chromosomal gene necessary for intimate attachment of enteropathogenic Escherichia coli to epithelial cells. J Bacteriol 175: 4670-4680, 1993.
8. + exchanger isoforms NHE-1, NHE-2, and NHE-3 mRNA. Am J Physiol Gastrointest Liver Physiol 271: G483-G493, 1996.
9. Gauthier A, Puente JL, and Finlay BB. Secretin of the enteropathogenic Escherichia coli type III secretion system requires components of the type III apparatus for assembly and localization. Infect Immun 71: 3310-3319, 2003.
10. + exchange by serotonin in Caco-2 cells (Abstract). Gastroenterology 122: W950, 2002.
11. Gill RK, Saksena S, Syed IA, Tyagi S, Alrefai WA, Malakooti J, Ramaswamy K, and Dudeja PK. Regulation of NHE3 by nitric oxide in Caco-2 cells. Am J Physiol Gastrointest Liver Physiol 283: G747-G756, 2002.
12. Gonda T, Maouyo D, Rees SE, and Montrose MH. Regulation of intracellular pH gradients by identified Na/H exchanger isoforms and a short-chain fatty acid. Am J Physiol Gastrointest Liver Physiol 276: G259-G270, 1999.
13. Goosney DL, Gruenheid S, and Finlay BB. Gut feelings: enteropathogenic E. coli (EPEC) interactions with the host. Annu Rev Cell Dev Biol 16: 173-189, 2000.
14. Hecht G. Microbes and Microbial Toxins: Paradigms for Microbial-Mucosal Interactions. VII. Enteropathogenic Escherichia coli: physiological alterations from an extracellular position. Am J Physiol Gastrointest Liver Physiol 281: G1-G7, 2001.
15. -) exchange activity in Caco-2 cells (Abstract). Gastroenterology 124: A482, 2003.
16. - secretion. Am J Physiol Gastrointest Liver Physiol 276: G781-G788, 1999.
17. - secretion from intestinal epithelia by upregulating galanin-1 receptor expression. J Clin Invest 104: 253-262, 1999.
18. Ide T, Laarmann S, Greune L, Schillers H, Oberleithner H, and Schmidt MA. Characterization of translocation pores inserted into plasma membranes by type III-secreted Esp proteins of enteropathogenic Escherichia coli. Cell Microbiol 3: 669-679, 2001.
19. + exchanger isoform 3 in human colon adenocarcinoma cell line Caco-2. Protein kinase C-mediated inhibition of the exchanger. J Biol Chem 273: 8790-8798, 1998.
20. Jarvis KG, Giron JA, Jerse AE, McDaniel TK, Donnenberg MS, and Kaper JB. Enteropathogenic Escherichia coli contains a putative type III secretion system necessary for the export of proteins involved in attaching and effacing lesion formation. Proc Natl Acad Sci USA 92: 7996-8000, 1995.
21. Kenny B. Mechanism of action of EPEC type III effector molecules. Int J Med Microbiol 291: 469-477, 2002.
22. Kenny B and Jepson M. Targeting of an enteropathogenic Escherichia coli (EPEC) effector protein to host mitochondria. Cell Microbiol 2: 579-590, 2000.
23. Lai LC, Wainwright LA, Stone KD, and Donnenberg MS. A third secreted protein that is encoded by the enteropathogenic Escherichia coli pathogenicity island is required for transduction of signals and for attaching and effacing activities in host cells. Infect Immun 65: 2211-2217, 1997.
24. + exchanger has no apparent effect on diarrheal state of NHE3-deficient mice. Am J Physiol Gastrointest Liver Physiol 281: G1385-G1396, 2001.
25. Madara JL, Patapoff TW, Gillece-Castro B, Colgan SP, Parkos CA, Delp C, and Mrsny RJ. 5′-Adenosine monophosphate is the neutrophil-derived paracrine factor that elicits chloride secretion from T84 intestinal epithelial cell monolayers. J Clin Invest 91: 2320-2325, 1993.
26. Matkowskyj KA, Danilkovich A, Marrero J, Savkovic SD, Hecht G, and Benya RV. Galanin-1 receptor up-regulation mediates the excess colonic fluid production caused by infection with enteric pathogens. Nat Med 6: 1048-1051, 2000.
27. McNamara BP, Koutsouris A, O'Connell CB, Nougayrede JP, Donnenberg MS, and Hecht G. Translocated EspF protein from enteropathogenic Escherichia coli disrupts host intestinal barrier function. J Clin Invest 107: 621-629, 2001.
28. + exchangers NHE2 and NHE3 in intestinal epithelial cell line C2/bbe. Am J Physiol Cell Physiol 275: C693-C701, 1998.
29. Menestrina G, Moser C, Pellet S, and Welch R. Pore formation by Escherichia coli hemolysin (HlyA) and other members of the RTX toxins family. Toxicology 87: 249-267, 1994.
30. Musch MW, Bookstein C, Xie Y, Sellin JH, and Chang EB. SCFA increase intestinal Na absorption by induction of NHE3 in rat colon and human intestinal C2/bbe cells. Am J Physiol Gastrointest Liver Physiol 280: G687-G693, 2001.
31. Nemeth ZH, Deitch EA, Lu Q, Szabo C, and Hasko G. NHE blockade inhibits chemokine production and NF-κB activation in immunostimulated endothelial cells. Am J Physiol Cell Physiol 283: C396-C403, 2002.
32. + exchanger blockade inhibits enterocyte inflammatory response and protects against colitis. Am J Physiol Gastrointest Liver Physiol 283: G122-G132, 2002.
33. + exchangers NHE2 and NHE3 in rat intestine and human Caco-2/bbe cells. Am J Physiol Cell Physiol 280: C1224-C1232, 2001.
34. Savkovic SD, Koutsouris A, and Hecht G. Attachment of a noninvasive enteric pathogen, enteropathogenic Escherichia coli, to cultured human intestinal epithelial monolayers induces transmigration of neutrophils. Infect Immun 64: 4480-4487, 1996.
35. Savkovic SD, Koutsouris A, and Hecht G. Activation of NF-κB in intestinal epithelial cells by enteropathogenic Escherichia coli. Am J Physiol Cell Physiol 273: C1160-C1167, 1997.
36. Savkovic SD, Ramaswamy A, Koutsouris A, and Hecht G. EPEC-activated ERK1/2 participate in inflammatory response but not tight junction barrier disruption. Am J Physiol Gastrointest Liver Physiol 281: G890-G898, 2001.
37. Shull GE, Miller ML, and Schultheis PJ. Lessons from Genetically Engineered Animal Models. VIII. Absorption and secretion of ions in the gastrointestinal tract. Am J Physiol Gastrointest Liver Physiol 278: G185-G190, 2000.
38. Spitz J, Yuhan R, Koutsouris A, Blatt C, Alverdy J, and Hecht G. Enteropathogenic Escherichia coli adherence to intestinal epithelial monolayers diminishes barrier function. Am J Physiol Gastrointest Liver Physiol 268: G374-G379, 1995.
39. Stein MA, Mathers DA, Yan H, Baimbridge KG, and Finlay BB. Enteropathogenic Escherichia coli markedly decreases the resting membrane potential of Caco-2 and HeLa human epithelial cells. Infect Immun 64: 4820-4825, 1996.
40. + exchanger isoform by the cytoskeleton. Cell Physiol Biochem 10: 265-272, 2000.
41. Tu X, Nisan I, Yona C, Hanski E, and Rosenshine I. EspH, a new cytoskeleton-modulating effector of enterohaemorrhagic and enteropathogenic Escherichia coli. Mol Microbiol 47: 595-606, 2003.
42. +-glucose cotransport in intestinal epithelia. Am J Physiol Cell Physiol 281: C1533-C1541, 2001.
43. + exchanger gene family: structure and function studies. Am J Physiol Gastrointest Liver Physiol 269: G1-G11, 1995.