Method for detection and enumeration of Cryptosporidium parvum oocysts in feces, manures, and soils

Applied and Environmental Microbiology

Volumn 65, Issue 7, 1999, Pages 2820-2826

  Kuczynska, Ewa ^a   Shelton, Daniel R ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CRYPTOSPORIDIUM PARVUM; FECES ANALYSIS; MANURE; NONHUMAN; OOCYST; PARASITE IDENTIFICATION; SOIL;

ANIMALIA; BOVINAE; CERVIDAE; CRYPTOSPORIDIUM PARVUM; EQUUS CABALLUS; GALLUS GALLUS; OVIS ARIES; PROTOZOA; SUS SCROFA;

EID: 0033019980     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.65.7.2820-2826.1999     Document Type: Article

References (32)

1. Anguish, L. J., and W. C. Ghiorse. 1997. Computer-assisted laser scanning and video microscopy for analysis of Cryptosporidium parvum oocysts in soil, sediment, and feces. Appl. Environ. Microbiol. 63:724-733.
2. Arrowood, M. J., and C. R. Sterling. 1987. Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic percoll gradients. J. Parasitol. 73:314-319.
3. Atwill, E. R., R. A. Sweitzer, M. Perira, I. Gardiner, D. van Vuren, and W. Boyce. 1997. Prevalence of and associated risk factors for shedding Cryptosporidium oocysts and Giardia cysts within feral pig populations in California. Appl. Environ. Microbiol. 63:3946-3949.
4. Bloem, J., P. R. Bolhuis, M. R. Veninga, and J. Wieringa. 1995. Microscopic methods for counting bacteria and fungi in soil, p. 162-173. In A. Kassem and P. Nannipieri (ed.), Methods in applied soil microbiology and biochemistry. Academic Press, New York, N.Y.
5. Bukhari, Z., and H. U. Smith. 1995. Effect of three concentration techniques on viability of Cryptosporidium parvum oocysts recovered from bovine feces. J. Clin. Microbiol. 33:2592-2595.
6. Chichino, G., A. Bruno, C. Cevini, C. Atzori, S. Gatti, and M. Scaglia. 1991. New rapid staining methods of Cryptosporidium oocysts in stools. J. Protozool. 38:212S-214S.
7. Environmental Protection Agency. 1995. Proposed general NPDES permit for concentrated animal feeding operations (CAFO). Fed. Reg. 60:44489-44498.
8. Fayer, R., T. K. Graczyk, E. J. Lewis, J. M. Trout, and C. A. Farley. 1998. Survival of infectious Cryptosporidium parvum oocysts in seawater and eastern oysters (Crassostrea virginica) in the Chesapeake Bay. Appl. Environ. Microbiol. 64:1070-1074.
9. Fayer, R., and W. Ellis. 1993. Paromomycin is effective as prophylaxis for cryptosporidiosis in dairy calves. J. Parasitol. 79:771-774.
10. Garber, L. P., M. D. Salman, H. S. Hurd, T. Keefe, and J. L. Schlater. 1994. Potential risk factors for Cryptosporidium infection in dairy calves. J. Am. Vet. Med. Assoc. 205:86-91.
11. Hansen, J. S., and J. E. Ongerth. 1991. Effects of time and watershed characteristics on the concentration of Cryptosporidium oocysts in river water. Appl. Environ. Microbiol. 57:2790-2795.
12. Kilani, R., and L. Sekla. 1987. Purification of Cryptosporidium oocysts and sporozoites by cesium chloride and Percoll gradients. Am. J. Trop. Med. Hyg. 36:505-508.
13. MacKenzie, W. R., N. J. Hoxie, M. E. Proctor, M. S. Gradus, K. A. Blair, D. E. Peterson, J. J. Kazmierczak, D. A. Addiss, K. R. Fox, J. R. Rose, and J. P. Davis. 1994. A massive outbreak of Cryptosporidium infection transmitted through the public water supply. N. Engl. J. Med. 331:161-167.
14. Mawdsley, J. L., A. E. Brooks, and R. J. Merry. 1996. Movement of the protozoan pathogen Cryptosporidium parvum through three contrasting soil types. Biol. Fertil. Soils 21:30-36.
15. Mtambo, M. M. A., A. S. Nash, D. A. Blewett, and S. Wright 1992. Comparison of staining and concentration techniques for detection of Cryptosporidium oocysts in cat faecal specimens. Vet. Parasitol. 45:49-57.
16. Ong, C., W. Moorehead, A. Ross, and J. Isaac-Renton. 1996. Studies of Giardia spp. and Cryptosporidium spp. in two adjacent watersheds. Appl. Environ. Microbiol. 62:2798-2805.
17. Peeters, J., and I. Villacarta. 1995. Crypiosporidium, p. 220. In J. Eckert, R. Braun, M. W. Shirley, and P. Coudert (ed.), Guidelines on techniques in coccidiosis research. European Commission Directorate-General XII, Science, Research and Development Environment Research Program.
18. Scott, C. A., H. V. Smith, and H. A. Gibbs, 1994. Excretion of Crypiosporidium parvum oocysts by a herd of beef suckler cows. Vet. Rec. 134:172.
19. States, S., K. Stadterman, L. Ammon, P. Vogel, J. Baldizar, D. Wright, L. Conley, and J. Sykora. 1997. Protozoa in river water: sources, occurrence, and treatment. J. Am. Water Works Assoc. 74:75-83.
20. Suresh, P., and R. Jerold. 1996. Comparative evaluation of several techniques for purification of Cryptosporidium parvum oocysts from rat feces. J. Clin. Microbiol. 34:38-40.
21. Tacal, V., Jr., M. Sobieh, and A. El-ahraf. 1987. Cryptosporidium in market pigs in southern California, USA. Vet. Rec. 120:615-617.
22. Vetterling, J. 1969. Continuous-flow differential density flotation of coccidial oocysts and a comparison with other methods. J. Parasitol. 55:412-417.
23. Walker, M. J., C. Montemagno, J. C. Bryant, and W. Ghiorse. 1998. Method detection limits of PCR and immunofluorescence assay for Cryptosporidium parvum in soil. Appl. Environ. Microbiol. 64:2281-2283.
24. Weber, R., B. Ralph, H. Bishop, S. Wahlquist, J. Sullivan, and D. Juranek. 1991. Threshold of detection of Cryptosporidium oocysts in human stool specimens: evidence for low sensitivity of current diagnostic methods. J. Clin. Microbiol. 29:1323-1327.
25. Weber, R., R. T. Bryan, and D. D. Juranek, 1992. Improved stool concentration procedure for detection of Cryptosporidium oocysts in fecal specimens. J. Clin. Microbiol. 30:2869-2873.
26. Webster, K. A., H. V. Smith, M. Giles, L. Dawson, and L. J. Robertson. 1996. Detection of Cryptosporidium parvum oocysts in feces: comparison of conventional coproscopical methods and the polymerase chain reaction. Vet. Parasitol. 61:5-13.
27. Weil, R. R. 1989. A laboratory manual for general soils, 4th ed., p. 23-32. Ginn Press, Needham Heights, Mass.
28. Xiao, L., and R. P. Herd. 1993. Quantitation of Giardia and Cryptosporidium oocysts in fecal samples by direct immunonuorescence assay. J. Clin. Microbiol. 31:2944-2956.
29. Xiao, L., and R. P. Herd. 1994. Review of equine Cryptosporidium infection. Equine Vet. J. 26:9-13.
30. Xiao, L., R. P. Herd, and K. E. McClure. 1994. Periparturient rise in the excretion of Giardia sp. cysts and Cryptosporidium parvum oocysts as a source of infection for lambs. J. Parasitol. 80:55-59.
31. Young, K. H., S. L. Bullock, D. M. Melvin, and C. L. Sprivil. 1979. Ethylacetate as a substitute for diethylether in the formalin-ether sedimentation technique. J. Clin. Microbiol. 10:852-853.
32. Zlerdt, W. 1984. Concentration and identification of Cryptosporidium sp. by use of a parasite concentrator. J. Clin. Microbiol. 20:860-861.