Modulation of 5′ splice site selection using tailed oligonucleotides carrying splicing signals

BMC Biotechnology

Volumn 6, Issue , 2006, Pages

  Gendron, Daniel ^a   Carriero, Sandra ^b   Garneau, Daniel ^a   Villemaire, Jonathan ^a   Klinck, Roscoe ^a   Elela, Sherif Abou ^a   Damha, Masad J ^b   Chabot, Bernoit ^a  

^a UNIVERSITY OF SHERBROOKE   (Canada)

^b MCGILL UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; BIOTECHNOLOGY; HEMOGLOBIN; NUCLEIC ACID SEQUENCES;

ANTISENSE TECHNOLOGY; GLOBIN BRANCH SITE SEQUENCE; INHIBITORY ACTIVITY; OLIGONUCLEOTIDES; RNA SPLICING; SPLICING INHIBITION; TAIL STRUCTURE;

RNA;

BETA GLOBIN; MESSENGER RNA; OLIGONUCLEOTIDE; PROTEIN BCL XL; RIBONUCLEOPROTEIN;

ARTICLE; BINDING SITE; CONTROLLED STUDY; GENE TECHNOLOGY; HUMAN; HUMAN CELL; IN VITRO STUDY; MODULATION; NUCLEIC ACID PROBE; NUCLEOTIDE SEQUENCE; PROCESS DEVELOPMENT; RNA SPLICING; RNA STRUCTURE; SIGNAL TRANSDUCTION;

EID: 33644543767     PISSN: 14726750     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-6-5     Document Type: Article

References (40)

1. Johnson JM, Castle J, Garrett-Engele P, Kan Z, Loerch PM, Armour CD, Santos R, Schadt EE, Stoughton R, Shoemaker DD: Genomewide survey of human alternative pre-mRNA splicing with exon junction microarrays. Science 2003, 302:2141-2144.
2. Wu JY, Tang H, Havlioglu N: Alternative pre-mRNA splicing and regulation of programmed cell death. Prog Mol Subcell Biol 2003, 31:153-185.
3. Jiang ZH, Wu JY: Alternative splicing and programmed cell death. Proc Soc Exp Biol Med 1999, 220:64-72.
4. Boise LH, Gonzalez-Garcia M, Postema CE, Ding L, Lindsten T, Turka LA, Mao X, Nunez G, Thompson CB: bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell death. Cell 1993, 74:597-608.
5. Tu Y, Renner S, Xu F, Fleishman A, Taylor J, Weisz J, Vescio R, Rettig M, Berenson J, Krajewski S, Reed JC, Lichtenstein A: BCL-X expression in multiple myeloma: possible indicator of chemoresistance. Cancer Res 1998, 58:256-262.
6. Reeve JG, Xiong J, Morgan J, Bleehen NM: Expression of apoptosis-regulatory genes in lung tumour cell lines: relationship to p53 expression and relevance to acquired drug resistance. Br J Cancer 1996, 73:1193-1200.
7. Olopade OI, Adeyanju MO, Safa AR, Hagos F, Mick R, Thompson CB, Recant WM: Overexpression of BCL-x protein in primary breast cancer is associated with high tumor grade and nodal metastases. Cancer J Sci Am 1997, 3:230-237.
8. Krajewska M, Krajewski S, Epstein JI, Shabaik A, Sauvageot J, Song K, Kitada S, Reed JC: Immunohistochemical analysis of bcl-2, bax, bcl-X, and mcl-1 expression in prostate cancers. Am J Pathol 1996, 148:1567-1576.
9. Clarke MF, Apel IJ, Benedict MA, Eipers PG, Sumantran V, Gonzalez-Garcia M, Doedens M, Fukunaga N, Davidson B, Dick JE, et al.: A recombinant bcl-x s adenovirus selectively induces apoptosis in cancer cells but not in normal bone marrow cells. Proc Natl Acad Sci U S A 1995, 92:11024-11028.
10. Ealovega MW, McGinnis PK, Sumantran VN, Clarke MF, Wicha MS: bcl-xs gene therapy induces apoptosis of human mammary tumors in nude mice. Cancer Res 1996, 56:1965-1969.
11. Mercatante DR, Bortner CD, Cidlowski JA, Kole R: Modification of alternative splicing of Bcl-x pre-mRNA in prostate and breast cancer cells. analysis of apoptosis and cell death. J Biol Chem 2001, 276:16411-16417.
12. Taylor JK, Zhang QQ, Monia BP, Marcusson EG, Dean NM: Inhibition of Bcl-xL expression sensitizes normal human keratinocytes and epithelial cells to apoptotic stimuli. Oncogene 1999, 18:4495-4504.
13. Sumantran VN, Ealovega MW, Nunez G, Clarke MF, Wicha MS: Overexpression of Bcl-XS sensitizes MCF-7 cells to chemotherapy-induced apoptosis. Cancer Res 1995, 55:2507-2510.
14. Sazani P, Kole R: Modulation of alternative splicing by antisense oligonucleotides. Prog Mol Subcell Biol 2003, 31:217-239.
15. Sazani P, Gemignani F, Kang SH, Maier MA, Manoharan M, Persmark M, Bortner D, Kole R: Systemically delivered antisense oligomers upregulate gene expression in mouse tissues. Nat Biotechnol 2002, 20:1228-1233.
16. Scaffidi P, Misteli T: Reversal of the cellular phenotype in the premature aging disease Hutchinson-Gilford progeria syndrome. Nat Med 2005, 11:440-445.
17. Villemaire J, Dion I, Elela SA, Chabot B: Reprogramming alternative pre-messenger RNA splicing through the use of protein-binding antisense oligonucleoticies. J Biol Chem 2003, 278:50031-50039.
18. Patry C, Bouchard L, Labrecque P, Gendron D, Lemieux B, Toutant J, Lapointe E, Wellinger R, Chabot B: Small interfering RNA-mediated reduction in heterogeneous nuclear ribonucleoparticule A1/A2 proteins induces apoptosis in human cancer cells but not in normal mortal cell lines. Cancer Res 2003, 63:7679-7688.
19. Kamma H, Portman DS, Dreyfuss G: Cell type-specific expression of hnRNP proteins. Exp Cell Res 1995, 221:187-196.
20. Kramer A: Purification of splicing factor SFI, a heat-stable protein that functions in the assembly of a presplicing complex. Mol Cell Biol 1992, 12:4545-4552.
21. Chabot B, Steitz JA: Multiple interactions between the splicing substrate and small nuclear ribonucleoproteins in spliceosomes. Mol Cell Biol 1987, 7:281-293.
22. Carriero S, Damha MJ: Inhibition of pre-mRNA splicing by synthetic branched nucleic acids. Nucleic Acids Res 2003, 31:6157-6167.
23. Barabino SM, Blencowe BJ, Ryder U, Sproat BS, Lamond AI: Targeted snRNP depletion reveals an additional role for mammalian UI snRNP in spliceosome assembly. Cell 1990, 63:293-302.
24. Barabino SM, Sproat BS, Ryder U, Blencowe BJ, Lamond AI: Mapping U2 snRNP--pre-mRNA interactions using biotinylated oligonucleotides made of 2′-OMe RNA. EMBO J 1989, 8:4171-4178.
25. Ruskin B, Green MR: An RNA processing activity that debranches RNA lariats. Science 1985, 229:135-140.
26. Pebernard S, Iggo RD: Determinants of interferon-stimulated gene induction by RNAi vectors. Differentiation 2004, 72:103-111.
27. Sledz CA, Holko M, de Veer MJ, Silverman RH, Williams BR. Activation of the interferon system by short-interfering RNAs. Nat Cell Biol 2003, 5:834-839.
28. Persengiev SP, Zhu X, Green MR: Nonspecific, concentration-dependent stimulation and repression of mammalian gene expression by small interfering RNAs (siRNAs). RNA 2004, 10:12-18.
29. Rossi F, Labourier E, Forne T, Divita G, Derancourt J, Riou JF, Antoine E, Cathala G, Brunel C, Tazi J: Specific phosphorylation of SR proteins by mammalian DNA topoisomerase I. Nature 1996, 381:80-82.
30. Skordis LA, Dunckley MG, Yue B, Eperon IC, Muntoni F: Bifunctional antisense oligonucleotides provide a trans-acting splicing enhancer that stimulates SMN2 gene expression in patient fibroblasts. Proc Natl Acad Sci U S A 2003, 100:4114-4119.
31. Cartegni L, Krainer AR: Correction of disease-associated exon skipping by synthetic exon-specific activators. Nat Struct Biol 2003, 10:120-125.
32. Usman N, Ogilvie KK, Jiang MY, Cedergren RG: Automated chemical synthesis of long oligoribonucleotides using 2′-Osilylated ribonucleoside 3′-O-phosphoramidites on a controlled-pore glass support: Synthesis of a 43-nucleotide sequence similar to the 3′-half molecule of Escherichia coli formylmethionine tRNA. J Am Chem Soc 1987,109:7845-7854.
33. Carriero S, Damha MJ: Solid-Phase Synthesis of Branched Oilgonucleotides. In Current Protocols in Nucleic Acids Chemistry Volume 4.14. Edited by: Beaucage SL, Glick GD, Bergstrom DE and Jones RA. New York, John Wiley & Sons; 2002.
34. Damha MJ, Zabarylo SV: Automated Solid-Phase Synthesis of Branched Oligonucleotides. Tetrahedron Letters 1989, 30:6295-6298.
35. Damha MJ, Ganeshan K, Hudson RH, Zabarylo SV. Solid-phase synthesis of branched oligoribonucleotides related to messenger RNA splicing intermediates. Nucleic Acids Res 1992, 20:6565-6573.
36. Damha MJ, Giannaris PA, Marfey P: Antisense L/D-oligodeoxynucleotide chimeras: nuclease stability, base-pairing properties, and activity at directing ribonuclease H. Biochemistry 1994, 33:7877-7885.
37. Garneau D, Revil T, Fisette JF, Chabot B: hnRNP F/H proteins modulate the alternative splicing of the apoptotic mediator Bcl-x. J Biol Chem 2005, 280:22641-22650.
38. Chabot B: Synthesis and purification of RNA substrates. In RNA processing, A practical approach Volume I. Edited by: Higgins SJHBD. Oxford, IRL Press; 1994:1-29.
39. Dignam JD, Lebovitz RM, Roeder RG: Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res 1983, 11:1475-1489.
40. Krainer AR, Maniatis T, Ruskin B, Green MR: Normal and mutant human beta-globin pre-mRNAs are faithfully and efficiently spliced in vitro. Cell 1984,36:993-1005.