Nonisotopic detection of microRNA using digoxigenin labeled RNA probes

Molecular and Cellular Probes

Volumn 20, Issue 1, 2006, Pages 1-4

  Ramkissoon, Shakti H ^a,b   Mainwaring, Lori A ^b   Sloand, Elaine M ^b   Young, Neal S ^b   Kajigaya, Sachiko ^b  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

^b NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

Author keywords

DIG; MicroRNA; Northern analysis; RNA oligonucleotide probe

Indexed keywords

DIGOXIGENIN; MICRORNA;

ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN CELL; ISOTOPE LABELING; LEUKEMIA CELL LINE; NORTHERN BLOTTING; PRIORITY JOURNAL; RNA PROBE;

BLOTTING, NORTHERN; CELL LINE; DIGOXIGENIN; HUMANS; MICRORNAS; MOLECULAR STRUCTURE; NUCLEIC ACID HYBRIDIZATION; RNA PROBES;

EID: 32844458331     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcp.2005.07.004     Document Type: Article

References (10)

1. N.C. Lau, L.P. Lim, E.G. Weinstein, and D.P. Bartel An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans Science 294 2001 858 862
2. M.T. McManus MicroRNAs and cancer Semin Cancer Biol 13 2003 253 258
3. J. Takamizawa, H. Konishi, K. Yanagisawa, S. Tomida, H. Osada, and H. Endoh Reduced expression of the let-7 microRNAs in human lung cancers in association with shortened postoperative survival Cancer Res 64 2004 3753 3756
4. J. Kim, A. Krichevsky, Y. Grad, G.D. Hayes, K.S. Kosik, and G.M. Church Identification of many microRNAs that copurify with polyribosomes in mammalian neurons Proc Natl Acad Sci USA 101 2004 360 365
5. S. Yekta, I.H. Shih, and D.P. Bartel MicroRNA-directed cleavage of HOXB8 mRNA Science 304 2004 594 596
6. G.A. Calin, C. Sevignani, C.D. Dumitru, T. Hyslop, E. Noch, and S. Yendamuri Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers Proc Natl Acad Sci USA 101 2004 2999 3004
7. G.A. Calin, C.G. Liu, C. Sevignani, M. Ferracin, N. Felli, and C.D. Dumitru MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias Proc Natl Acad Sci USA 101 2004 11755 11760
8. J. Chevalier, J. Yi, O. Michel, and X.M. Tang Biotin and Digoxigenin as labels for light and electron microscopy in situ hybridization probes: where to we stand? J Histochem Cytochem 45 1997 481 491
9. R.K. Parkin, M.J. Boeckh, V. Erard, M.L. Huang, and D. Myerson Specific delineation of BK polyomavirus in kidney tissue with a digoxigenin lableled DNA probe Molec Cell Probes 19 2005 87 92
10. M.S. McCabe, JB.. Power, A.M. de Laat, and M.R. Davey Detection of single copy genes in DNA from transgenic plants by nonradioactive Southern blot analysis Mol Biotech 7 1997 79 84