High-level autoenhanced expression of a single-copy gene in Escherichia coli: Overproduction of bacteriophage T7 protein kinase directed by T7 late genetic elements

Gene

Volumn 262, Issue 1-2, 2001, Pages 231-238

  Marchand, Isabelle ^a   Nicholson, Allen W ^b   Dreyfus, Marc ^a  

^a ECOLE NORMALE SUPÉRIEURE   (France)

^b WAYNE STATE UNIVERSITY   (United States)

Author keywords

E. coli expression system; mRNA Stability; Promoter strength; Protein phosphorylation; Translation

Indexed keywords

DNA; MESSENGER RNA; PROTEIN KINASE; RNA POLYMERASE; TRANSFER RNA;

ALLELE; ARTICLE; BACTERIOPHAGE; CARBOXY TERMINAL SEQUENCE; CELL LABELING; CHROMOSOME INSERTION; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; GENE MUTATION; GENE OVEREXPRESSION; GENETIC STABILITY; GENETIC TRANSCRIPTION; MOLECULAR CLONING; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEIN SYNTHESIS; RNA TRANSLATION; TRANSLATION INITIATION;

BACTERIOPHAGE T7; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; GENE DOSAGE; GENE EXPRESSION REGULATION, BACTERIAL; POINT MUTATION; PROMOTER REGIONS (GENETICS); PROTEIN-SERINE-THREONINE KINASES; RNA PROCESSING, POST-TRANSCRIPTIONAL; TRANSCRIPTION, GENETIC; VIRAL PROTEINS;

ESCHERICHIA COLI;

EID: 0035834929     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(00)00526-6     Document Type: Article

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