Expression of active human cytochrome P-450c21 (CYPXXIA2) in Escherichia coli and one-step purification by metal-affinity chromatography

Biokhimiya

Volumn 61, Issue 10, 1996, Pages 1758-1771

  Guzov, V M ^a   Zel'ko, I N ^a   Chudaev, M V ^a   Guzova, Yu A ^a   Chung, B Ch ^a   Usanov, S A ^a  

^a INSTITUTE OF BIOORGANIC CHEMISTRY   (Belarus)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CHROMATOGRAPHY; ENZYMES; ESCHERICHIA COLI; GENES; GENETIC ENGINEERING; HORMONES;

CYTOCHROMES; GENE EXPRESSION;

PROTEINS;

COMPLEMENTARY DNA; CYTOCHROME P450; STEROID 21 MONOOXYGENASE;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANIMAL; ARTICLE; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; MOUSE; NUCLEOTIDE SEQUENCE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; CYTOCHROME P-450 ENZYME SYSTEM; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HUMANS; MICE; MOLECULAR SEQUENCE DATA; PLASMIDS; STEROID 21-HYDROXYLASE; SUBSTRATE SPECIFICITY;

EID: 0030251511     PISSN: 03209725     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (45)