Expression of functionally active cytochrome b5 in Escherichia coli: Isolation, purification, and use of the immobilized recombinant heme protein for affinity chromatography of electron-transfer proteins

Biochemistry (Moscow)

Volumn 62, Issue 4, 1997, Pages 401-411

  Chudaev, M V ^a   Usanov, S A ^a  

^a INSTITUTE OF BIOORGANIC CHEMISTRY   (Belarus)

Author keywords

Affinity chromatography; Cytochrome b5; Expression in Escherichia coli; Ion exchange chromatographic purification

Indexed keywords

ESCHERICHIA COLI;

AMINOLEVULINIC ACID; CYTOCHROME B5; CYTOCHROME P450; MEMBRANE PROTEIN; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; BIOSYNTHESIS; CONFORMATION; ELECTRON TRANSPORT; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETICS; IMMUNOBLOTTING; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MUTAGENESIS; OXIDATION REDUCTION REACTION; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; RAT; SEQUENCE ANALYSIS; SPECTROPHOTOMETRY;

AMINOLEVULINIC ACID; ANIMALS; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; CYTOCHROME P-450 ENZYME SYSTEM; CYTOCHROMES B5; ELECTRON TRANSPORT; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; IMMUNOBLOTTING; MEMBRANE PROTEINS; MUTAGENESIS; NUCLEIC ACID CONFORMATION; OXIDATION-REDUCTION; PLASMIDS; RATS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS; SPECTROPHOTOMETRY;

EID: 0031110163     PISSN: 00062979     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (66)

1. Usanov, S. A., Chashchin, V. L., and Akhrem, A. A. (1990) in Molecular Mechanisms of Adrenal Steroidogenesis and Aspects of Regulation and Application (Ruckpaul, K., and Rein, H., eds.) Frontiers in Biotransformation, Vol. 3, Akademie-Verlag, Berlin, pp. 1-57.
2. Schenkman, J. B., and Greim, H. (eds.) (1993) Cytochrome P-450, Springer-Verlag.
3. Oshino, N. (1980) in Hepatic Cytochrome P450 Monooxygenase System (Schenkman, J. B., and Kupfer, D., eds.) Pergamon Press, New York, pp. 407-447.
4. Peterson, J. A., and Prough, R. A. (1987) in Cytochrome P-450. Structure, Mechanism, and Biochemistry (Ortiz Montellano, P. R., ed.) Plenum Press, New-York, London, pp. 89-117.
5. Ozols, J. (1976) Annals of Clin. Res., 8, 182-192.
6. Kozutsumi, Y., Kawano, T., Yamakawa, T., and Suzuki, A. (1990) J. Biochem., 108, 704-706.
7. Shaw, L., Schneckenburger, P., Schlenzka, W., Carlsen, J., Christiansen, K., Jurgensen, D., and Schauer, R. (1994) Eur. J. Biochem., 219, 1001-1011.
8. Takematsu, H., Kawano, T., Koyama, S., Kozutsumi, Y., Suzuki, A., and Kawasaki, T. (1994) J. Biochem., 115, 381-386.
9. Abe, K., and Sugita, Y. (1979) Eur. J. Biochem., 101, 423-428.
10. Enoch, H. G., and Strittmatter, P. (1979) J. Biol. Chem., 254, 8976-8981.
11. Tamburini, P. P., MacFarqubar, S., and Schenkman, J. B. (1986) Biochem. Biophys. Res. Commun., 134, 519-526.
12. Nisimoto, Y., and Otsuka-Murakami, H. (1988) Biochemistry, 27, 5869-5876.
13. Nishimoto, Y., and Lambeth, J. D. (1985) Arch. Biochem. Biophys., 241, 386-396.
14. Chiang, J. Y. L. (1981) Arch. Biochem. Biophys., 211, 622-673.
15. Miki, N., Sugiyama, T., and Yamano, T. (1980) J. Biochem., 88, 307-316.
16. Bendzko, P., Usanov, S. A., Pfeil, W., and Ruckpaul, K. (1982) Acta Biol. Med. Germ., 41, 1-8.
17. Usanov, S. A., Bendzko, P., Pfeil, W., Janig, G. R., and Ruckpaul, K. (1983) Bioorg. Khim., 9, 450-461.
18. Tamburini, P. P., Schenkman, J. B., and White, R. E. (1985) J. Biol. Chem., 260, 4007-4015.
19. Omata, Y., Sakamoto, H., Robinson, R. C., Pinkus, M. R., and Friedman, F. K. (1994) Biochem. Biophys. Res. Commun., 201, 1090-1095.
20. Omata, Y., Robinson, R. C., Gelboin, H. V., Pinkus, M. R., and Friedman, F. K. (1994) FEBS Lett., 346, 241-245.
21. Honkakoski, P., Linnala-Kankkunen, A., Usanov, S. A., and Lang, M. A. (1992) Biochim. Biophys. Acta, 1122, 6-14.
22. Juvonen, R. O., Iwasaki, M., and Negjshi, M. (1992) Biochemistry, 24, 11519-11523.
23. Stayton, P., Fisher, M. T., and Sugar, S. G. (1988) J. Biol. Chem., 263, 13544-13548.
24. Stayton, P., Poulos, T. L., and Sligar, S. G. (1989) Biochemistry, 28, 8201-8205.
25. Usanov, S. A., Chashchin, V. L., and Akhrem, A. A. (1989) Biokhimiya, 54, 472-486.
26. Usanov, S. A., and Chashchin, V. L. (1991) FEBS Lett., 278, 279-282.
27. Kominami, S., Ogawa, N., Morimune, R., De-Ying, H., and Takemori, S. (1992) J. Steroid Biochem. Mol. Biol., 42, 57-64.
28. Katagiri, M., Kagawa, N., and Waterman, M. R. (1995) Arch. Biochem. Biophys., 317, 343-347.
29. Salemme, F. R. (1976) J. Mol. Biol., 102, 563-568.
30. Ng, S., Smith, M. B., Smith, H. T., and Millett, F. (1977) Biochemistry, 16, 4975-4978.
31. Rodgers, K. K., Pochapsky, T. C., and Sligar, S. G. (1988) Science, 240, 1657-1659.
32. Eltis, L. D., Herbert, R. G., Barker, P. D., Mauk, A. G., and Northrup, S. H. (1991) Biochemistry, 30, 3663-3674.
33. Livigstone, D. J., McLachlan, S. J., La Mar, G. N., and Brown, W. D. (1985) J. Biol. Chem., 260, 15699-15707.
34. Dailey, H. A., and Strittmatter, P. (1979) J. Biol. Chem., 254, 5388-5396.
35. Spats, D., and Strittmatter, P. (1971) Proc. Natl. Acad. Sci. USA, 68, 1042-1046.
36. Abe, K., Kimura, S., Kizawa, R., Anan, F. K., and Sugita, Y. (1985) J. Biochem., 97, 1659-1668.
37. Ozols, J. (1989) Biochim. Biophys. Acta, 997, 121-130.
38. Yoo, M., and Steggles, A. W. (1988) Biochem. Biophys. Res. Comnan., 156, 576-580.
39. Zhang, H., and Somerville, C. (1988) Arch. Biochem. Biophys., 264, 343-347.
40. Beck von Bodman, S., Schuler, M. A., Jollie, D. R., and Sligar, S. G. (1986) Proc. Natl. Acad. Sci. USA, 83, 9443-9447.
41. Funk, W. D., Lo, T. P., Mauk, M. R., Braver, G. D., MacGillivray, R. T. A., and Mauk, A. G. (1990) Biochemistry, 29, 5500-5508.
42. Ladokhin, A. S., Wang, L., Steggles, A. W., and Holloway, P. W. (1991) Biochemistry, 30, 10200-10206.
43. Rivera, M., Barillas Mury, C., Christensen, K. A., Little, J. W., Wells, M. A., and Walker, F. A. (1992) Biochemistry, 31, 12233-12240.
44. Vergeres, G., and Waskell, L. (1992) J. Biol. Chem., 267, 12583-12591.
45. Hewson, R., Newbold, R. J., and Whitford, D. (1993) Protein Engineering, 6, 953-964.
46. Holmans, P. L., Shet, M. S., Martin-Wixtrom, C. A., Fisher, C. W., and Estabrook, R. W. (1994) Arch. Biochem. Biophys., 312, 554-565.
47. Lloyd, E., Ferrer, J. C., Funk, W. D., Mauk, M. R., and Mauk, A G. (1994) Biochemistry, 33, 11432-11437.
48. Truan, G., Cullin, C., Reisdorf, P., Urban, P., and Pompon, D. (1993) Gene, 125, 49-55.
49. Sligar, S. G., Filipovic, D., and Stayton, P. S. (1991) Meth. Enzymol., 206, 31-49.
50. Vergeres, G., Chen, D. Y., Wu, F. F., and Waskell, L. (1993) Arch. Biochem. Biophys., 305, 231-241.
51. Vergeres, G., and Waskell, L. (1992) J. Biol. Chem., 267, 12583-12591.
52. Peyronneau, M. A., Renaud, J. P., Truan, G., Urban, P., Pompon, D., and Mansuy, D. (1992) Eur. J. Biochem., 207, 109-116.
53. Bhattacharyya, A. K., Hurley, J. K., Tollin, G., and Waskell, L. (1994) Arch. Biochem. Biophys., 310, 318-324.
54. Mitoma, J., and Ito, A. (1992) The EMBO J., 11, 4197-4203.
55. Usanov, S. A., Kurchenko, V. P., and Metelitza, D. I. (1982) Bioorg. Khim., 8, 630-642.
56. Walker, J. F., Fearnley, I. M., and Blows, R. A. (1986) Biochem. J., 237, 73-84.
57. Black, S. D., and Coon, M. J. (1982) Analyt. Biochem., 121, 281-285.
58. Bruton, C. J., and Hartley, B. C. (1970) J. Mol. Biol., 52, 165-178.
59. Massey, V., Stankovich, M., and Hemmerich, P. (1978) Biochemistry, 17, 1-8.
60. Dawson, R., Elliott, D., Elliott, W., and Jones, K. (1991) Data for Biochemical Research [Russian translation], Mir, Moscow.
61. Laemlli, U. K. (1970) Nature, 227, 680-685.
62. Omura, T., and Sato, R. (1964) J. Biol. Chem., 239, 2370-2379.
63. Strittmatter, P., and Velick, S. P. (1956) J. Biol. Chem., 221, 253-264.
64. Barnes, H., Arlotto, M., and Waterman, M. R. (1991) Proc. Natl. Acad. Sci. USA, 88, 5597-5601.
65. Richardson, T. H., Yung, F., Griffin, K. J., Wester, M., Raucy, J. L., Kemper, B., Bornheim, L. M., Hassett, C., Omiecinski, C. J., and Johnson, E. F. (1995) Arch. Biochem. Biophys., 323, 87-96.
66. Guzov, V. M., Zelko, I. N., Chudaev, M. V., Guzova, Yu. A., Chung, B.-C., and Usanov, S. A. (1996) Biochemistry (Moscow), 61, 1758-1771 (Russ.).