Molecular cloning and heterologous expression in E. coli of cytochrome P45017α. Comparison of structural and functional properties of substrate-specific cytochromes P450 from different species

Biochemistry (Moscow)

Volumn 68, Issue 1, 2003, Pages 86-98

  Gilep, A A ^a   Estabrook, R W ^b   Usanov, S A ^a  

^a INSTITUTE OF BIOORGANIC CHEMISTRY   (Belarus)

^b UNIVERSITY OF TEXAS AT DALLAS   (United States)

Author keywords

Cytochrome P450; Cytochrome P45017 ; Heterologous expression; Protein protein interaction

Indexed keywords

AFFINITY CHROMATOGRAPHY; CLONING; CYTOLOGY; GENES; HYDROXYLATION; PURIFICATION; REACTION KINETICS; SUBSTRATES;

MOLECULAR CLONING;

ENZYMES;

BISON; BOVIDAE; BOVINAE; CAPRA HIRCUS; CAVIA PORCELLUS; CRICETINAE; OVIS ARIES; SUS SCROFA;

RECOMBINANT PROTEIN; STEROID 17ALPHA MONOOXYGENASE;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BUFFALO; CHEMICAL STRUCTURE; CHEMISTRY; COMPARATIVE STUDY; ENZYME SPECIFICITY; ESCHERICHIA COLI; GENE EXPRESSION; GENE VECTOR; GENETICS; GOAT; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROTEIN CONFORMATION; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; SHEEP; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BISON; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; GOATS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SHEEP; STEROID 17-ALPHA-HYDROXYLASE; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY;

EID: 0642369727     PISSN: 00062979     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1022101703670     Document Type: Article

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