Peptide nucleic acid clamp PCR: A novel K-ras mutation detection assay for colorectal cancer micrometastases in lymph nodes

International Journal of Cancer

Volumn 111, Issue 3, 2004, Pages 409-414

  Taback, Bret ^a   Bilchik, Anton J ^a   Saha, Sukamal ^b   Nakayama, Takahiro ^a   Wiese, David A ^c   Turner, Roderick R ^d   Kuo, Christine T ^a   Hoon, Dave S B ^a  

^a JOHN WAYNE CANCER INSTITUTE   (United States)

^b MICHIGAN STATE UNIVERSITY   (United States)

^c MICHIGAN STATE UNIVERSITY   (United States)

^d SAINT JOHN'S HEALTH CENTER   (United States)

Author keywords

Colorectal cancer; Metastases, K ras mutation; Peptide nucleic acid; Sentinel lymph node

Indexed keywords

EOSIN; HEMATOXYLIN; PEPTIDE NUCLEIC ACID;

ADULT; AGED; ARTICLE; CANCER STAGING; CLINICAL ARTICLE; CODON; COLORECTAL CANCER; DNA ISOLATION; FEMALE; GENE MUTATION; HUMAN; HUMAN CELL; HUMAN TISSUE; LYMPH NODE METASTASIS; MALE; ONCOGENE K RAS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SENTINEL LYMPH NODE; SEQUENCE ANALYSIS;

ADULT; AGED; AGED, 80 AND OVER; BASE SEQUENCE; CELL LINE, TUMOR; CODON; COLORECTAL NEOPLASMS; DNA PRIMERS; DNA, NEOPLASM; FEMALE; GENES, RAS; HUMANS; LYMPH NODES; LYMPHATIC METASTASIS; MALE; MIDDLE AGED; MUTATION; NEOPLASM STAGING; PEPTIDE NUCLEIC ACIDS; POINT MUTATION; POLYMERASE CHAIN REACTION;

EID: 3242882243     PISSN: 00207136     EISSN: None     Source Type: Journal    
DOI: 10.1002/ijc.20268     Document Type: Article

References (38)

1. Fearon ER, Vogelstein B. A genetic model for colorectal tumorigenesis. Cell 1990;61:759-67.
2. Arends JW. Molecular interactions in the Vogelstein model of colorectal carcinoma. J Pathol 2000;190:412-6.
3. Vogelstein B, Fearon ER, Hamilton SR, Kern SE, Preisinger AC, Leppert M, Nakamura Y, White R, Smits AM, Bos JL. Genetic alterations during colorectal-tumor development. N Engl J Med 1988;319:525-32.
4. Losi L, Benhattar J, Costa J. Stability of K-ras mutations throughout the natural history of human colorectal cancer. Eur J Cancer 1992;28:1115-20.
5. Dix BR, Robbins PD, Spagnolo DV, Padovan GL, House AK, Iacopetta BJ. Clonal analysis of colorectal tumors using K-ras and p53 gene mutations as markers. Diagn Mol Pathol 1995;4:261-5.
6. Shibata D, Schaeffer J, Li ZH, Capella G, Perucho M. Genetic heterogeneity of the c-K-ras locus in colorectal adenomas but not in adenocarcinomas. J Natl Cancer Inst 1993;85:1058-63.
7. Sorenson GD. Detection of mutated KRAS2 sequences as tumor markers in plasma/serum of patients with gastrointestinal cancer. Clin Cancer Res 2000;6:2129-37.
8. Erlichman C, Fine S, Wong A, Elhakim T. A randomized trial of fluorouracil and folinic acid in patients with metastatic colorectal carcinoma. J Clin Oncol 1988;6:469-75.
9. Cohen AM, Tremiterra S, Candela F, Thaler HT, Sigurdson ER. Prognosis of node-positive colon cancer. Cancer 1991;67:1859-61.
10. Jass JR, Miller K, Northover JM. Fat clearance method versus manual dissection of lymph nodes in specimens of rectal cancer. Int J Colorectal Dis 1986;1:155-6.
11. Cawthorn SJ, Gibbs NM, Marks CG. Clearance technique for the detection of lymph nodes in colorectal cancer. Br J Surg 1986;73:58-60.
12. Scott KW, Grace RH. Detection of lymph node metastases in colorectal carcinoma before and after fat clearance. Br J Surg 1989;76:1165-7.
13. Schmitz-Moormann P, Thomas C, Pohl C, Sohl R. Patho-anatomical demonstration of lymph node metastases in a surgical specimen. Pathol Res Pract 1982;174:403-11.
14. Wong JH, Severino R, Honnebier MB, Tom P, Namiki TS. Number of nodes examined and staging accuracy in colorectal carcinoma. J Clin Oncol 1999;17:2896-900.
15. Cutait R, Alves VA, Lopes LC, Cutait DE, Borges JL, Singer J, da Silva JH, Goffi FS. Restaging of colorectal cancer based on the identification of lymph node micrometastases through immunoperoxidase staining of CEA and cytokeratins. Dis Colon Rectum 1991;34:917-20.
16. Adell G, Boeryd B, Franlund B, Sjodahl R, Hakansson L. Occurrence and prognostic importance of micrometastases in regional lymph nodes in Dukes' B colorectal carcinoma: an immunohistochemical study. Eur J Surg 1996;162:637-42.
17. Greenson JK, Isenhart CE, Rice R, Mojzisik C, Houchens D, Martin EW, Jr. Identification of occult micrometastases in pericolic lymph nodes of Duke's B colorectal cancer patients using monoclonal antibodies against cytokeratin and CC49. Correlation with long-term survival. Cancer 1994;73:563-9.
18. Jeffers MD, O'Dowd GM, Mulcahy H, Stagg M, O'Donoghue DP, Toner M. The prognostic significance of immunohistochemically detected lymph node micrometastases in colorectal carcinoma. J Pathol 1994;172:183-7.
19. Morton DL, Wen DR, Wong JH, Economou JS, Cagle LA, Storm FK, Foshag LJ, Cochran AJ. Technical details of intraoperative lymphatic mapping for early stage melanoma. Arch Surg 1992;127:392-9.
20. Giuliano AE, Kirgan DM, Guenther JM, Morton DL. Lymphatic mapping and sentinel lymphadenectomy for breast cancer. Ann Surg 1994;220:391-8.
21. Bilchik AJ, Giuliano A, Essner R, Bostick P, Kelemen P, Foshag LJ, Sostrin S, Turner RR, Morton DL. Universal application of intraoperative lymphatic mapping and sentinel lymphadenectomy in solid neoplasms. Cancer J Sci Am 1998;4:351-8.
22. Turner RR, Ollila DW, Krasne DL, Giuliano AE. Histopathologic validation of the sentinel lymph node hypothesis for breast carcinoma. Ann Surg 1997;226:271-6.
23. Ray A, Norden B. Peptide nucleic acid (PNA): its medical and biotechnical applications and promise for the future. Faseb J 2000;14:1041-60.
24. Uhlmann E. Peptide nucleic acids (PNA) and PNA-DNA chimeras: from high binding affinity towards biological function. Biol Chem 1998;379:1045-52.
25. Thiede C, Bayerdorffer E, Blasczyk R, Wittig B, Neubauer A. Simple and sensitive detection of mutations in the ras proto-oncogenes using PNA-mediated PCR clamping. Nucleic Acids Res 1996;24:983-4.
26. Taback B, Chan AD, Kuo CT, Bostick PJ, Wang HJ, Giuliano AE, Hoon DS. Detection of occult metastatic breast cancer cells in blood by a multimolecular marker assay: correlation with clinical stage of disease. Cancer Res 2001;61:8845-50.
27. Sanchez-Cespedes M, Esteller M, Hibi K, Cope FO, Westra WH, Piantadosi S, Herman JG, Jen J, Sidransky D. Molecular detection of neoplastic cells in lymph nodes of metastatic colorectal cancer patients predicts recurrence. Clin Cancer Res 1999;5:2450-4.
28. Hayashi N, Ito I, Yanagisawa A, Kato Y, Nakamori S, Imaoka S, Watanabe H, Ogawa M, Nakamura Y. Genetic diagnosis of lymph-node metastasis in colorectal cancer. Lancet 1995;345:1257-9.
29. Thebo JS, Senagore AJ, Reinhold DS, Stapleton SR. Molecular staging of colorectal cancer: K-ras mutation analysis of lymph nodes upstages Dukes B patients. Dis Colon Rectum 2000;43:155-9.
30. Noura S, Yamamoto H, Miyake Y, Kim B, Takayama O, Seshimo I, Ikenaga M, Ikeda M, Sekimoto M, Matsuura N, Monden M. Immunohistochemical assessment of localization and frequency of micro-metastases in lymph nodes of colorectal cancer. Clin Cancer Res 2002;8:759-67.
31. Wood TF, Tsioulias GJ, Morton DL, Rangel D, Hutchinson W, Jr., Foshag LJ, Turner RR, Bilchik AJ. Focused examination of sentinel lymph nodes upstages early colorectal carcinoma. Am Surg 2000;66:998-1003.
32. Tortola S, Steinert R, Hantschick M, Peinado MA, Gastinger I, Stosiek P, Lippert H, Schlegel W, Reymond MA. Discordance between K-ras mutations in bone marrow micrometastases and the primary tumor in colorectal cancer. J Clin Oncol 2001;19:2837-43.
33. Giaretti W, Monaco R, Pujic N, Rapallo A, Nigro S, Geido E. Intratumor heterogeneity of K-ras2 mutations in colorectal adenocarcinomas: association with degree of DNA aneuploidy. Am J Pathol 1996;149:237-45.
34. Al-Mulla F, Going JJ, Sowden ET, Winter A, Pickford IR, Birnie GD. Heterogeneity of mutant versus wild-type Ki-ras in primary and metastatic colorectal carcinomas, and association of codon-12 valine with early mortality. J Pathol 1998;185:130-8.
35. Kinzler KW, Vogelstein B. Colorectal Tumors. In: Kinzler KW, Vogelstein B, eds. The genetic basis of human cancer. New York: McGraw-Hill, 1998.
36. Yun K, Merrie AE, Gunn J, Phillips LV, McCall JL. Keratin 20 is a specific marker of submicroscopic lymph node metastases in colorectal cancer: validation by K-RAS mutations. J Pathol 2000;191:21-6.
37. Bostick PJ, Chatterjee S, Chi DD, Huynh KT, Giuliano AE, Cote R, Hoon DS. Limitations of specific reverse-transcriptase polymerase chain reaction markers in the detection of metastases in the lymph nodes and blood of breast cancer patients. J Clin Oncol 1998;16:2632-40.
38. Andreyev HJ, Norman AR, Cunningham D, Oates J, Dix BR, Iacopetta BJ, Young J, Walsh T, Ward R, Hawkins N, Beranek M, Jandik P. Kirsten ras mutations in patients with colorectal cancer: the "RASCAL II" study. Br J Cancer 2001;85:692-6.