Probing for pH-regulated proteins in Sinorhizobium medicae using proteomic analysis

Journal of Molecular Microbiology and Biotechnology

Volumn 7, Issue 3, 2004, Pages 140-147

  Reeve, Wayne G ^a   Tiwari, Ravi P ^a   Guerreiro, Nelson ^b   Stubbs, Janine ^b   Dilworth, Michael J ^a   Glenn, Andrew R ^c   Rolfe, Barry G ^b   Djordjevic, Michael A ^b   Howieson, John G ^a  

^a MURDOCH UNIVERSITY   (Australia)

^b AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

^c UNIVERSITY OF TASMANIA   (Australia)

Author keywords

Acid response; Acidity; Low pH; Proteomic analysis; Sinorhizobium

Indexed keywords

ABC TRANSPORTER; ACID; BACTERIAL PROTEIN; ENDOPEPTIDASE CLP; FRUCTOSE BISPHOSPHATE ALDOLASE; LIPOPROTEIN; MALATE DEHYDROGENASE; PROTEIN DEGP; PROTEIN GROES; PROTEIN WRBA1; UNCLASSIFIED DRUG;

ACIDITY; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL CELL; BACTERIAL GENETICS; CONTROLLED STUDY; DOWN REGULATION; GENETIC TRANSCRIPTION; GRAM NEGATIVE BACTERIUM; NONHUMAN; PH; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN DETERMINATION; PROTEIN TRANSPORT; PROTEOMICS; REGULATORY MECHANISM; SINORHIZOBIUM; SINORHIZOBIUM MEDICAE;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PROTEOME; SINORHIZOBIUM;

SINORHIZOBIUM; SINORHIZOBIUM MEDICAE;

EID: 3242787381     PISSN: 14641801     EISSN: None     Source Type: Journal    
DOI: 10.1159/000078657     Document Type: Article

References (28)

1. Castanie-Cornet, M.P, Penfound, T.A., Smith, D., Elliott, J.F., Foster, J.W. 1999. Control of acid resistance in Escherichia coli . J. Bacteriol. 181:3525-3535.
2. Chen, H., Higgins, J., Oresnik, I.J., Hynes, M.F., Natera, S., Djordjevic, M.A., Weinman, J.J., Rolfe, B.G. 2000. Proteome analysis demonstrates complex replicon and luteolin interactions in pSymA-cured derivatives of Sinorhizobium meliloti strain 2011. Electrophoresis 21:3833-3842.
3. Djordjevic, M.A., Chen, H.C., Natera, S., Van Noorden, G., Menzel, C., Taylor, S., Renard, C., Geiger, O., Weiller, G.F. 2003. A global analysis of protein expression profi les in Sinorhizobium meliloti: Discovery of new genes for nodule occupancy and stress adaptation. Mol. Plant Microbe Interact. 16:508-524.
4. Foster, J.W., Moreno, M. 1999. Inducible acid tolerance mechanisms in enteric bacteria; in Cardew, G. (ed.): Bacterial Response to pH. Novartis Found. Symp. 221. Wiley Publishing, London, pp. 55-74.
5. Goss, T.G., O'Hara, G.W., Dilworth, M.J., Glenn, A.R. 1990. Cloning, characterization, and complementation of lesions causing acid sensitivity in Tn5-induced mutants of Rhizobium meliloti WSM419. J. Bacteriol. 172:5173-5179.
6. Gottesman, S. 1999. Regulation by proteolysis: Developmental switches. Curr. Opin. Microbiol. 2:142-147.
7. Grandori, R., Khalifah, P., Boice, J.A., Fairman, R., Giovanielli, K., Carey, J. 1998. Biochemical characterization of WrbA, founding member of a new family of multimeric flavodoxin-like proteins. J. Biol. Chem. 273:20960-20966.
8. Guerreiro, N., Djordjevic, M.A., Rolfe, B.G. 1999. Proteome analysis of the model microsymbiont Sinorhizobium meliloti: Isolation and characterisation of novel proteins. Electrophoresis 20:818-825.
9. Guerreiro, N., Redmond, J.W., Rolfe, B.G., and Djordjevic, M.A. 1997. New Rhizobium leguminosarum flavanoid-induced proteins revealed by proteome analysis of differentially displayed proteins. Mol. Plant Microbe Interact. 10:506-516.
10. Hengge-Aronis, R. 1996. Regulation of gene expression during entry into stationary phase; in Neidhardt, F.C. (chief ed.): Escherichia coli and Salmonella. Cellular and Molecular Biology. ASM Press, Washington, pp. 1497-1512.
11. Howieson, J.G., Ewing, M.A. 1986. Acid tolerance in the Rhizobium meliloti-Medicago symbiosis. Aust. J. Agric. Res. 37:55-64.
12. Howieson, J.G., Ewing, M.A. 1989. Annual species of Medicago differ greatly in their ability to nodulate on acid soils. Aust. J. Agric. Res. 40:843-850.
13. Howieson, J.G., Ewing, M.A., D'Antuono, M.F. 1988. Selection for acid tolerance in Rhizobium meliloti . Plant Soil 105:179-188.
14. Lemos, J.A., Chen, Y.Y., Burne, R.A. 2001. Genetic and physiological analysis of the groE operon and role of the HrcA repressor in stress gene regulation and acid tolerance in Streptococcus mutans . J. Bacteriol. 183:6074-6084.
15. Lund, P.A. 2001. Microbial molecular chaperones. Adv. Microb. Physiol. 44:93-140.
16. Matsudaira, P. 1987. Sequence from picomole quantities of proteins electroblotted onto PVDF membranes. J. Biol. Chem. 262:10035-10038.
17. Natera, S.H.A., Guerreiro, N., Djordjevic, M.A. 2000. Proteome analysis of differentially displayed proteins as a tool for the investigation of symbiosis. Mol. Plant Microbe Interact. 13:995-1009.
18. Nouwens, A.S., Cordwell, S.J., Larsen, M.R., Molloy, M.P., Gillings, M., Willcox, M.D.P., Walsh, B.J. 2000. Complementing genomics with proteomics: The membrane sub-proteome of Pseudomonas aeruginosa PAO1. Electrophoresis 21:3797-3809.
19. O'Hara, G.W., Goss, T.J., Dilworth, M.J., Glenn, A.R. 1989. Maintenance of intracellular pH and acid tolerance in Rhizobium meliloti. Appl. Environ. Microbiol. 55:1870-1876.
20. Rabilloud, T. 1992. A comparison between low background silver diammine and silver nitrate protein stains. Electrophoresis 13:429-439.
21. Reeve, W.G., Tiwari, R.P., Wong, C.M., Dilworth, M.J., Glenn, A.R. 1998. The transcriptional regulator gene phrR in Sinorhizobium meliloti WSM419 is regulated by pH and other stresses. Microbiology 144:3335-3342.
22. Reeve, W.G., Tiwari, R.P., Worsley, P.S., Dilworth, M.J., Glenn, A.R., Howieson, J.G. 1999. Constructs for insertional mutagenesis, transcriptional signal localisation and gene regulation studies in root nodule and other bacteria. Microbiology 145:1307-1316.
23. Tiwari, R.P., Reeve, W.G., Dilworth, M.J., Glenn, A.R. 1996a. An essential role for actA in acid tolerance of Rhizobium meliloti. Microbiology 142:601-610.
24. Tiwari, R.P., Reeve, W.G., Dilworth, M.J., Glenn, A.R. 1996b. Acid tolerance in Rhizobium meliloti. WSM419 involves a two-component sensor-regulator system. Microbiology 142:1693-1704.
25. Tiwari, R.P., Reeve, W.G., Fenner, B.J., Dilworth, M.J., Glenn, A.R., Howieson, J.G. 2004. Probing for pH-Regulated Genes in Sinorhizobium medicae using Transcriptional Analysis. J Mol Microbiol Biotechnol 7:133-139.
26. Tiwari, R.P., Reeve, W.G., Glenn, A.R. 1992. Mutations conferring acid sensitivity in the acidtolerant strains Rhizobium meliloti WSM419 and Rhizobium leguminosarum biovar viciae WSM710. FEMS Microbiol. Lett. 100:107-112.
27. Wilkins, M.R., Gasteiger, E., Sanchez, J., Bairoch, A., Hochstrasser, D.F. 1998. Two-dimensional gel electrophoresis for proteome projects: The effects of protein hydrophobicity and copy number. Electrophoresis 19:1501-1505.
28. Yang, W., Ni, L., Somerville, R.L. 1993. A stationary phase protein of Escherichia coli that affects the mode of association between the trp repressor protein and operator-bearing DNA. Proc. Natl. Acad. Sci. USA 90:5796-5800.