Constructs for insertional mutagenesis, transcriptional signal localization and gene regulation studies in root nodule and other bacteria

Microbiology

Volumn 145, Issue 6, 1999, Pages 1307-1316

  Reeve, Wayne G ^a   Tiwari, Ravi P ^a   Worsley, Penelope S ^a   Dilworth, Michael J ^a   Glenn, Andrew R ^b   Howieson, John G ^a  

^a MURDOCH UNIVERSITY   (Australia)

^b UNIVERSITY OF TASMANIA   (Australia)

Author keywords

gusA; Induction; phr genes; Reporter; Rhizobium

Indexed keywords

ANTIBIOTIC AGENT; GENTAMICIN; KANAMYCIN;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL MUTATION; DNA FLANKING REGION; DNA SEQUENCE; ESCHERICHIA COLI; GENE CASSETTE; GENE CONTROL; GENE INACTIVATION; GENE INSERTION; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; REGULATOR GENE; REPORTER GENE; STOP CODON; TRANSPOSON;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; RHIZOBIUM;

EID: 0033053645     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/13500872-145-6-1307     Document Type: Article

References (29)

1. Becker, A., Schmidt, M., Jager, W. & Puhler, A. (1995). New gentamicin-resistance and lacZ promoter-probe cassettes suitable for insertion mutagenesis and generation of transcriptional fusions. Gene 162, 37-39.
2. Berg, D. E. & Howe, M. M. (1989). Mobile DNA. Washington, DC: American Society for Microbiology.
3. Bethesda Research Laboratories (1986). BRL pUC host: E. coli DH5α™ competent cells. Focus 8, 9.
4. Boyer, H. W. & Roulland-Dussoix, D. (1969). A complementation analysis of the restriction and modification of DNA in Escherichia colt. J Mol Biol 41, 459-472.
5. Fellay, R., Frey, J. & Krisch, H. (1987). Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of Gram-negative bacteria. Gene 52, 147-154.
6. Figurski, D. H. & Helinski, D. R. (1979). Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc Natl Acad Sci USA 76, 1648-1652.
7. Glenn, A. R., Tiwari, R. P., Reeve, W. G. & Dilworth, M. J. (1997). The response of root nodule bacteria to acid stress. In Plant-Soil Interactions at Low pH, pp. 123-138. Edited by A. C. Moniz, A. M. C. Furlani, R. E. Schaffert, N. K. Fageria, C. A. Rosolen & H. Cantarella. Campinas, Brazil : Brazilian Soil Science Society.
8. Goss, T. G., O'Hara, G. W., Dilworth, M. J. & Glenn, A. R. (1990). Cloning, characterization, and complementation of lesions causing acid sensitivity in Tn5-induced mutants of Rhizobium meliloti WSM419. J Bacteriol 172, 5173-5179.
9. Herrero, M., dè Lorenzo, V. & Timmis, K. N. (1990). Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in Gram-negative bacteria. J Bacteriol 172, 6557-6567.
10. Howieson, J. G., Loi, A. & Carr, S. J. (1995). Biserrula pelecinus L. - a legume pasture species with potential for acid, duplex soils which is nodulated by unique root-nodule bacteria. Aust J Agric Res 46, 997-1009.
11. Inoue, H., Nojima, H. & Okayama, H. (1990). High efficiency transformation of Escherichia coli with plasmids. Gene 96, 23-28.
12. Jefferson, R. A., Burgess, S. M. & Hirsh, D. (1986). β-Glucoronidase from Escherichia coli as a gene-fusion marker. Proc Natl Acad Sci USA 83, 8447-8451.
13. de Lorenzo, V., Herrero, M., Jakubzik, U. & Timmis, K. N. (1990). Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in Gram-negative eubacteria. J Bacteriol 172, 6568-6572.
14. Metcalf, W. W., Jiang, W., Daniels, L. L., Kim, S., Haldimann, A. & Wanner, B. L. (1996). Conditionally replicative and conjugative plasmids carrying lacZa for cloning, mutagenesis and allele replacement in bacteria. Plasmid 35, 1-13.
15. Miller, J. H. (1972). Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
16. O'Hara, G. W., Goss, T. J., Dilworth, M. J. & Glenn, A. R. (1989). Maintenance of intracellular pH and acid-tolerance in Rhizobium meliloti. Appl Environ Microbiol 55, 1870-1876.
17. Reeve, W. G., Tiwari, R. P., Dilworth, M. J. & Glenn, A. R. (1997). A helicase gene (helO) in Rhizobium meliloti WSM419. FEMS Microbiol Lett 153, 43-49.
18. Reeve, W. G., Tiwari, R. P., Wong, C. M., Dilworth, M. J. & Glenn, A. R. (1998). The transcriptional regulator gene phrR in Sinorhizobium meliloti WSM419 is regulated by low pH and other stresses. Microbiology 144, 3335-3342.
19. Reznikoff, W. S. (1993). The Tn5 transposon. Annu Rev Microbiol 47, 945-963.
20. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY : Cold Spring Harbor Laboratory.
21. Sharma, S. B. & Signer, E. R. (1990). Temporal and spatial regulation of the symbiotic genes of Rhizobium meliloti in planta revealed by transposon Tn5-gusA. Genes Dev 4, 344-356.
22. Silhavy, T. J. & Beckwith, J. R. (19B5). Uses of lac fusions for the study of biological problems. Microbiol Rev 49, 398-418.
23. Simons, R. W., Houman, F. & Kleckner, N. (1987). Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene 53, 85-96.
24. Spaink, H. P., Robert, J. H., Okker, C. A., Wijffelman, E. P. & Lugtenberg, B. J. J. (1987). Promoters in the nodulation region of the Rhizobium leguminosarum Sym plasmid pRL1JI. Plant Mol Biol 9, 27-39.
25. Tiwari, R. P., Reeve, W. G., Dilworth, M. J. & Glenn, A. R. (1996a). An essential role for act A in acid-tolerance of Rhizobium meliloti. Microbiology 142, 601-610.
26. Tiwari, R. P., Reeve, W. G., Dilworth, M. J. & Glenn, A. R. (1996b). Acid-tolerance in Rhizobium meliloti strain WSM419 involves a two-component sensor-regulator system. Microbiology 142, 1693-1704.
27. Vieira, J. & Messing, J. (1991). New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene 100, 189-194.
28. Wilson, K. J., Sessitsch, A., Corbo, J. C., Giller, K. E., Akkermans, A. D. L. & Jefferson, R. A. (1995). β-Glucuronidase (GUS) transposons for ecological and genetic studies of rhizobia and other Gram-negative bacteria. Microbiology 141, 1691-1705.
29. Yanisch-Perron, C., Vieira, J. & Messing, J. (1985). Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103-119.