Differential gene expression of fibroblasts: Keloid versus normal

International Journal of Molecular Sciences

Volumn 3, Issue 11-12, 2002, Pages 1162-1176

  Cohly, Hari H P ^a   Scott, Heath ^a   Ndebele, Kenneth ^a   Jenkins, John K ^a   Angel, Michael F ^a  

^a UNIVERSITY OF MISSISSIPPI SCHOOL OF MEDICINE   (United States)

Author keywords

CDNA expression arrays; Keloid dermal fibroblasts; Normal dermal fibroblasts; RNA fingerprinting

Indexed keywords

BETA ACTIN; COMPLEMENTARY DNA; CYCLIC AMP DEPENDENT PROTEIN KINASE; DNA BINDING PROTEIN; HEAT SHOCK PROTEIN 27; HEAT SHOCK PROTEIN 90; PRIMER DNA; RIBOSOME PROTEIN; THIOREDOXIN PEROXIDASE; TRANSCRIPTION FACTOR; TUBULIN;

ARTICLE; CELL PROLIFERATION; CONTROLLED STUDY; DOWN REGULATION; FIBROBLAST; GENE CONTROL; GENE EXPRESSION; HUMAN; HUMAN CELL; KELOID; RNA FINGERPRINTING; UPREGULATION;

EID: 3242746005     PISSN: 14220067     EISSN: None     Source Type: Journal    
DOI: 10.3390/i3111162     Document Type: Article

References (44)

1. Tredget, E. E.; Nedelec, B.; Scott, P. G.; Ghahary, A. Hypertrophic scars, keloids, and contractures. The cellular and molecular basis for therapy. Surg. Clin. North Am. 1997, 77, 701-730.
2. Alhady, S. M.; Sivanantharajah, K. Keloids in various races. A review of 175 cases. Plast. & Reconstr. Surg. 1969, 44, 564-566.
3. Peacock, E. E. Jr.; Madden, J. W.; Trier, W. C. Biologic basis for the treatment of keloids and hypertrophic scars. South Med. J. 1970, 63, 755-760.
4. Tuan, T. L.; Nichter, L. S. The molecular basis of keloid and hypertrophic scar formation. Mol. Med. Today 1998, 4, 19-24.
5. Canman, C. E.; Gilmer, T. M.; Coutts, S. B.; Kastan, M. B. Growth factor modulation of p53-mediated growth arrest versus apoptosis. Genes Dev. 1995, 9, 600-611.
6. Ladin, D. A.; Hou, Z.; Patel, D.; McPhail, M.; Olson, J. C.; Saed, G. M.; Fivenson, D. P. p53 and apoptosis alterations in keloids and keloid fibroblasts. Wound Repair Regen. 1998, 6, 28-37.
7. Sayah, D. N.; Soo, C.; Shaw, W. W.; Watson, J.; Messadi, D.; Longaker, M. T.; Zhang, X.; Ting, K. Downregulation of apoptosis-related genes in keloid tissues. J. Surg. Res. 1999, 87, 209-216.
8. McCoy, B. J., Cohen, I. K. Effects of various sera on growth kinetics and collagen synthesis by keloid and normal dermal fibroblasts. Plast. Reconstr. Surg. 1981, 67, 505-510.
9. Shah, M.; Foreman, D. M.; Ferguson, M. W. Neutralizing antibody to TGF-beta 1,2 reduces cutaneous scarring in adult rodents. J. Cell Sci. 1994, 107, 1137-1157.
10. Shah, M.; Foreman, D. M.; Ferguson, M. W. Neutralization of TGF-beta 1 and TGF-beta 2 or exogenous addition of TGF-beta 3 to cutaneous rat wounds reduces scarring. J. Cell Sci. 1995, 108, 985-1002.
11. Polo, M.; Ko, F.; Busillo, F.; Cruse, C. W.; Krizek, T. J.; Robson, M. C. The 1997 Moyer Award. Cytokine production in patients with hypertrophic burn scars. J. Burn Care Rehabil. 1997, 18, 477-482.
12. Polo, M.; Smith, P. D.; Kim, Y. J.; Wang, X.; Ko, F.; Robson, M. C. Effect of TGF-beta2 on proliferative scar fibroblast cell kinetics. Ann. Plast. Surg. 1999, 43, 185-190.
13. Arcari, P.; Martinelli, R.; Salvatore, F. The complete sequence of a full-length cDNA for human liver glyceraldehyde-3-phosphate-dehydrogenase: evidence for multiple mRNA species. Nucleic Acid Res. 1984, 12, 9179-9189.
14. Ponte, P.; Ng, S. Y.; Engel, J.; Gunning, P.; Kedes, L. Nucleotide, Protein Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a human beta-actin cDNA. Nucleic Acids Res. 1984, 12, 1687-1696.
15. Cowan, N. J.; Dobner, P. R.; Fuchs, E. V.; Cleveland, D. W. Expression of human alpha-tubulin genes: interspecies conservation of 3′ untranslated regions. Mol. Cell Biol. 1983, 3, 1738-1745.
16. Carper, S. W.; Rocheleau, T. A.; Storm, F. K. cDNA sequence of a human heat shock protein HSP27. Nucleic Acids Res. 1990, 12, 9179-9189.
17. Zaman, G. J. Sequence of a cDNA encoding human ribosomal protein L26 and of a cDNA probably encoding human ribosomal protein L6. Nucleic Acids Res. 1993, 21, 1673.
18. Prosperi, M. T.; Ferbus, D.; Karczinski, I.; Goubin, G. A human cDNA corresponding to a gene overexpressed during cell proliferation encodes a product sharing homology with amoebic and bacterial proteins. J. Biol. Chem. 1993, 268, 11050-11056.
19. Kolluri, R., Kinniburgh, A. J. Full length cDNA sequence encoding a nuclease-sensitive element DNA binding protein. Nucleic Acids Res. 1991, 19, 4771.
20. De Morais, S. M.; Schweikl, H.; Blaisdell, J. Gene structure and upstream regulatory regions of human CYP2C9 and CYP2C18. Biochem. Biophys. Res. Commun. 1993, 194, 194-201.
21. Solberg, R.; Tasken, K.; Keiserud, A.; Jahnsen, T. Molecular cloning, cDNA structure and tissue-specific expression of the human regulatory subunit RI beta of cAMP-dependent protein kinases. Biochem. Biophys. Res. Commun. 1991, 176, 166-172
22. Hoffmann, T., Hovemann, B. Heat-shock proteins, Hsp 84, Hsp 86, of mice and men: two related genes encode formerly identified tumor-specific transplantation antigens. Gene 1988, 74, 491-501.
23. Smith, P.; Siegler, K.; Wang, X.; et al. TGF-B2 increases DNA synthesis and collagen production in keloid fibroblasts. Surg. Forum 1998, 16, 677.
24. Nakaoka, H.; Miyauchi, S.; Miki, Y. Proliferating activity of dermal fibroblasts in keloids and hypertrophic scars. Acta Derm. Venereol. 1995, 75, 102-104.
25. Duncan, M. R.; Hasan, A.; Berman, B. Oncostatin M stimulates collagen and glycosaminoglycan production by cultured normal dermal fibroblasts: insensitivity of sclerodermal and keloidal fibroblasts. J. Invest. Dermatol. 1995, 104, 128-133.
26. Lee, K. S.; Jung, J. B.; Ro, Y. J., et al. Effects of x-irradiation on survival and extracellular matrix gene expression of cultured keloid fibroblasts. J. Dermatol. Sci. 1994, 8, 33-37.
27. Szulgit, G.; Rudolph, R.; Wandel, A.; Tenenhaus, M.; Panos, R.; Gardner, H. Alterations in fibroblast alpha1beta1 integrin collagen receptor expression in keloids and hypertrophic scars. J. Invest. Dermatol. 2002, 118, 409-415.
28. Appleton, I.; Brown, N. J.; Willoughby, D. A. Apoptosis, necrosis and proliferation: Possible implications in the etiology of keloids. Am. J. Pathol. 1996, 149, 1441-1447.
29. Greco, R. M.; Icono, J. A.; Ehrlich, H. P. Hyaluronic acid stimulates human dermal fibroblast proliferation within a collagen matrix. J. Cellular Phys. 1998, 177, 65-73.
30. Prescott, A. R.; Vestberg, M.; Warn, R. M. Microtubulesrich in modified alpha-tubulin characterize the tail processes of motile fibroblasts. J. Cell Sci. 1989, 94, 227-236.
31. Mehlen, P.; Schulze-Osthoff, K.; Arrigo, A. P. Small stress proteins as novel regulators of apoptosis. Heat shock protein 27 blocks Fas/APO-1 and staurosporine-induced cell death. J. Biol. Chem. 1996, 271, 16510-16514.
32. Teofoli, P.; Barduagni, S.; Ribuffo, M.; Campanella, A.; De Pita, O.; Puddu, P. Expression of Bcl-2, p53, c-jun and c-fos protooncogenes in keloids and hypertrophic scars. J. Dermatol. Sci. 1999, 22, 31-37.
33. Davies, C.; Bussiere, D. E.; Golden, B. L.; Porter, S. J.; Ramakrishnan, V.; White, S. W. Ribosomal proteins S5 and L6: high-resolution crystal structures and roles in protein synthesis and antibiotic resistance. J. Mol. Biol. 1998, 279, 873-888.
34. Bjornstedt, M.; Kumar, S.; Bjorkhem, L.; Spyrou, G.; Holmgren, A. Selenium and the thioredoxin and glutaredoxin systems. Biomed. Environ. Sci. 1997, 10, 271-279.
35. Jin, D. Y.; Chae, H. Z.; Rhee, S. G.; Jeang, K. T. Regulatory role for a novel human thioredoxin peroxidase in NF-kappaB activation. J. Biol. Chem. 1997, 272, 30952-30961.
36. Ichimiya, S.; Davis, J. G.; O'Rourke, D. M.; Katsumata, M.; Greene, M. I. Murine thioredoxin peroxidase delays neuronal apoptosis and is expressed in areas of the brain most susceptible to hypoxic and ischemic injury. DNA Cell Biol. 1997, 16, 311-21.
37. Hoffman, E. K.; Trusko, S. P.; Murphy, M.; George, D. L. An S1 nuclease-sensitive homopurine/homopyrimidine domain in the c-Ki-ras promoter interacts with a nuclear factor. Proc. Natl. Acad. Sci. U S A 1990, 87, 2705-2709.
38. Johnson, A. C.; Jino, Y.; Merlino, G. T. Modulation of epidermal growth factor receptor protooncogene transcription by a promoter site sensitive to S1 nuclease. Mol. Cell Biol. 1988, 8, 4174-4184.
39. Postel, E. H.; Berberich, S. J.; Flint, S. J.; Ferrone, C. A. Human c-myc transcription factor PuF identified as nm23-H2 nucleoside diphosphate kinase, a candidate suppressor of tumor metastasis. Science 1993, 261, 478-80.
40. Ramakrishnan, N.; Kalinich, J.; McClain, D. E. Ebselon inhibition of apoptosis by reduction of peroxides. Biochem. Pharm. 1996, 51, 1443-1451.
41. Liu, A. Y.; Lin, Z.; Choi, H. S.; Sorhage, F.; Li, B. Attenuated induction of heat shock gene expression in aging diploid fibroblasts. J. Biol. Chem. 1989, 264, 12037-12045.
42. Chin, G. S.; Liu, W.; Steinbrech, D.; Hsu, M.; Levinson, H.; Longaker, M. T. Cellular signaling by tyrosine phosphorylation in keloid and normal human dermal fibroblasts. Plast. Reconstr. Surg. 2000, 106, 1532-40.
43. Chin, G. S.; Liu, W.; Peled, Z.; Lee, T. Y.; Steinbrech, D. S.; Hsu, M.; Longaker, M. T. Differential expression of transforming growth factor-beta receptors I and II and activation of Smad 3 in keloid fibroblasts. Plast. Reconstr. Surg. 2001, 108, 423-429.
44. Calderon, M.; Lawrence, W. T.; Banes, A. J. Increased proliferation of keloid fibroblasts in vitro. J. Surg. Res. 1996, 61, 343-7.