Phosphorylation of the enteropathogenic E. coli receptor by the Src-family kinase c-Fyn triggers actin pedestal formation

Nature Cell Biology

Volumn 6, Issue 7, 2004, Pages 618-625

  Phillips, Neil ^a   Hayward, Richard D ^a   Koronakis, Vassilis ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN RELATED PROTEIN 2; ACTIN RELATED PROTEIN 3; ADAPTOR PROTEIN; BACTERIAL PROTEIN; INTIMIN; PROTEIN KINASE FYN; PROTEIN TYROSINE KINASE; PROTEIN TYROSINE KINASE INHIBITOR; SMALL INTERFERING RNA; TYROSINE KINASE RECEPTOR; WISKOTT ALDRICH SYNDROME PROTEIN;

ACTIN POLYMERIZATION; ARTICLE; CELL CULTURE; CONTROLLED STUDY; ENZYME INHIBITION; ESCHERICHIA COLI; HOST PATHOGEN INTERACTION; HUMAN; HUMAN CELL; IN VITRO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENESIS; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; RNA INTERFERENCE; SIGNAL TRANSDUCTION;

ADHESINS, BACTERIAL; ANIMALS; CARRIER PROTEINS; CELL SURFACE EXTENSIONS; CELLS, CULTURED; ENZYME INHIBITORS; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; ESCHERICHIA COLI PROTEINS; FIBROBLASTS; MICE; MICROFILAMENTS; NIH 3T3 CELLS; ONCOGENE PROTEINS; PHOSPHORYLATION; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-FYN; RECEPTOR PROTEIN-TYROSINE KINASES; RECEPTORS, CELL SURFACE; RNA INTERFERENCE; SIGNAL TRANSDUCTION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 3242737673     PISSN: 14657392     EISSN: None     Source Type: Journal    
DOI: 10.1038/ncb1148     Document Type: Article

References (28)

1. Nataro, J. P. & Kaper, J. B. Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11, 142-201 (1998).
2. Celli, J., Deng, W. & Finlay, B. B. Enteropathogenic Escherichia coli (EPEC) attachment to epithelial cells; exploiting the host cell cytoskeleton from the outside. Cell. Microbiol. 2, 1-9 (2000).
3. Kenny, B. et al. Enteropathogenic E. coli (EPEC) transfers its receptor for intimate adherence into mammalian cells. Cell 91, 511-520 (1997).
4. Kenny, B. Phosphorylation of tyrosine 474 of the enteropathogenic Escherichia coli (EPEC) Tir receptor molecule is essential for actin nucleating activity and is preceded by additional host modifications. Mol. Microbiol. 31, 1229-1241 (1999).
5. Campellone, K. G., Giese, A., Tipper, D. J. & Leong, J. M. A tyrosine-phosphorylated 12-amino-acid sequence of enteropathogenic Escherichia coli Tir binds the host adaptor protein Nck and is required for Nck localisation to actin pedestals. Mol. Microbiol. 43, 1227-1241 (2002).
6. Gruenheid, S. et al. Enteropathogenic E. coli Tir binds Nck to initiate actin pedestal formation in host cells. Nature Cell Biol. 3, 856-859 (2001).
7. Kalman, D. et al. Enteropathogenic E. coli acts through WASP and Arp2/3 complex to form actin pedestals. Nature Cell Biol. 1, 389-391 (1999).
8. Touzé, T., Hayward, R. D., Eswaran, J., Leong, J. M. & Koronakis, V. Self-association of EPEC intimin mediated by the β-barrel-containing anchor domain: a role in clustering of the Tir receptor. Mol. Microbiol. 51, 73-87 (2004).
9. Liu, H., Magoun, L., Luperchio, S., Schauer, D. B. & Leong, J. M. The Tir-binding region of enterhaemorrhagic Escherichia coli intimin is sufficient to trigger actin condensation after bacterial-induced host cell signalling. Mol. Microbiol. 34, 67-81 (1999).
10. Rosenshine, I., Donnenberg, M. S., Kaper, J. B. & Finlay, B. B. Signal transduction between enteropathogenic Escherichia coli (EPEC) and elpithelial cells: EPEC induces tyrosine phosphorylation of host cell proteins to initiate cytoskeletal rearrangement and bacterial uptake. EMBO J. 11, 3551-3560 (1992).
11. Campellone, K. G. et al. Clustering of Nck by a 12-residue Tir phosphopeptice is sufficient to trigger localised actin assembly. J. Cell Biol. 164, 407-416 (2004).
12. Thomas, S. M. & Brugge, J. S. Cellular functions regulated by Src family kinases. Annu. Rev. Cell Dev. Biol. 13, 513-609 (1997).
13. Songyang, Z. et al. Catalytic specificity of protein-tyrosine kinases is critical for selective signalling. Nature 373, 536-539 (1995).
14. Cudmore, S., Cossart, P., Griffiths, G. & Way, M. Actin-based motility of Vaccinia virus. Nature 378, 636-638 (1995).
15. Selbach, M., Moese, S., Hauck, C. R., Meyer, T. F. & Backert, S. Src is the kinase of the Helicobacter pylori CagA protein in vitro and in vivo. J. Cell Biol. 277, 6775-6778(2002).
16. Hanke, J. H. et al. Discovery of a novel, potent, and Src family-selective tyrosine kinase inhibitor. Study of Lck- and FynT-dependent T cell activation. J. Biol. Chem. 271, 695-701 (1996).
17. Bain, J., McLauchlan, H., Elliott, M. & Cohen, P. The specificities of protein kinase inhibitors: an update. Biochem. J. 371, 199-204 (2003).
18. Klinghoffer, R. A., Sachsenmaier, C., Cooper, J. A. & Soriano, P. Src family kinases are required for integrin but not PDGFR signal transduction. EMBO J. 18, 2459-2471(1999).
19. Goosney, D. L., DeVinney, R. & Finlay, B. B. Recruitment of cytoskeletal and signalling proteins to enteropathogenic and enterohemorrhagic Escherichia coli pedestals. Infect. Immun. 69, 3315-3322 (2001).
20. Hayward, R. D. & Koronakis, V. Direct modulation of the host cell cytoskeleton by Salmonella actin-binding proteins. Trends Cell Biol. 21, 15-20 (2002).
21. Klausner, R. D. & Samelson, L. E. T cell antigen receptor activation pathways: the tyrosine kinase connection. Cell 64, 875-878 (1991).
22. Gauen, L. K. et al. Interactions of p59fyn and ZAP-70 with T-cell receptor activation motifs: defining the nature of a signalling motif. Mol. Cell. Biol. 14, 3729-3741 (1994).
23. Plattner, R., Kadlec, L., DeMali, K. A., Kazlauskas, A. & Pendergast A. M. c-Abl is activated by growth factors and Src family kinases and has a role in the cellular response to PDGF. Genes Dev. 13, 2400-2411 (1999).
24. Ilangumaran, S., Arni, S., van Echten-Deckert, G., Borisch, B. & Hoessli, D. C. Microdomain-dependent regulation of Lck and Fyn protein-tyrosine kinases in T lymphocyte plasma membranes. Mol. Biol. Cell 10, 891-905 (1999).
25. Simons, K. & Ikonen, E. Functional rafts in cell membranes. Nature 387, 569-572 (1997).
26. Foger, N., Marhaba, R. & Zoller, M. Involvement of CD44 in cytoskeleton rearrangement and raft reorganisation in T cells. J. Cell Sci. 114, 1169-1178 (2001).
27. Samelson, L. E., Phillips, A. F., Luong, E. T. & Klausner, R. D. Association of the Fyn protein-tyrosine kinase with the T-cell antigen receptor Proc. Natl Acad. Sci. USA 87, 4358-4362 (1990).
28. Zobiack, N. et al. Cell-surface attachment of pedestal-forming enteropathogenic E. coli induces a clustering of raft components and a recruitment of annexin 2. J. Cell Sci. 115, 91-98 (2002).