The state of the actin cytoskeleton determines its association with gephyrin: Role of ena/VASP family members

Molecular and Cellular Neuroscience

Volumn 31, Issue 2, 2006, Pages 376-386

  Bausen, Melanie ^a   Fuhrmann, Jens C ^a,b   Betz, Heinrich ^a   O'Sullivan, Gregory A ^a  

^a MAX PLANCK INSTITUTE FOR BRAIN RESEARCH   (Germany)

^b UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

Author keywords

Actin; Alkaloid; Cytoskeleton; Ena; Gephyrin; Inhibitory synapses; VASP

Indexed keywords

ACTIN; CYTOCHALASIN D; F ACTIN; G ACTIN; GEPHYRIN; VASODILATOR STIMULATED PHOSPHOPROTEIN;

ACTIN FILAMENT; ACTIN POLYMERIZATION; ARTICLE; BRAIN MATURATION; CELL DISRUPTION; CONTROLLED STUDY; CYTOSKELETON; HIPPOCAMPUS; HUMAN; HUMAN CELL; MICROFILAMENT; NEUROTRANSMISSION; POSTSYNAPTIC MEMBRANE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN FAMILY; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION;

ACTINS; ANIMALS; ANTINEOPLASTIC AGENTS; BICYCLO COMPOUNDS, HETEROCYCLIC; CARRIER PROTEINS; CELL ADHESION MOLECULES; CELLS, CULTURED; CYTOCHALASIN D; CYTOSKELETON; DEPSIPEPTIDES; HIPPOCAMPUS; HUMANS; MEMBRANE PROTEINS; MICROFILAMENT PROTEINS; MICROFILAMENTS; NEURONS; NUCLEIC ACID SYNTHESIS INHIBITORS; PHOSPHOPROTEINS; PROTEIN STRUCTURE, TERTIARY; RATS; RECOMBINANT FUSION PROTEINS; SYNAPSES; THIAZOLES; THIAZOLIDINES;

ENA; HYPSOPHRYS NICARAGUENSIS;

EID: 32244449287     PISSN: 10447431     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcn.2005.11.004     Document Type: Article

References (35)

1. M. Ackermann, and A. Matus Activity-induced targeting of profilin and stabilization of dendritic spine morphology Nat. Neurosci. 6 2003 1194 1200
2. D.W. Allison, A.S. Chervin, V.I. Gelfand, and A.M. Craig Postsynaptic scaffolds of excitatory and inhibitory synapses in hippocampal neurons: maintenance of core components independent of actin filaments and microtubules J. Neurosci. 20 2000 4545 4554
3. J.E. Bear, T.M. Svitkina, M. Krause, D.A. Schafer, J.J. Loureiro, G.A. Strasser, I.V. Maly, O.Y. Chaga, J.A. Cooper, G.G. Borisy, and F.B. Gertler Antagonism between Ena/VASP proteins and actin filament capping regulates fibroblast motility Cell 109 2002 509 521
4. M. Fischer, S. Kaech, D. Knutti, and A. Matus Rapid actin-based plasticity in dendritic spines Neuron 20 1998 847 854
5. J.C. Fuhrmann, S. Kins, P. Rostaing, O. El Far, J. Kirsch, M. Sheng, A. Triller, H. Betz, and M. Kneussel Gephyrin interacts with dynein light chains 1 and 2, components of motor protein complexes J. Neurosci. 22 2002 5393 5402
6. T. Giesemann, G. Schwarz, R. Nawrotzki, K. Berhorster, M. Rothkegel, K. Schluter, N. Schrader, H. Schindelin, R.R. Mendel, J. Kirsch, and B.M. Jockusch Complex formation between the postsynaptic scaffolding protein gephyrin, profilin, and Mena: a possible link to the microfilament system J. Neurosci. 23 2003 8330 8339
7. C. Haffner, T. Jarchau, M. Reinhard, J. Hoppe, S.M. Lohmann, and U. Walter Molecular cloning, structural analysis and functional expression of the proline-rich focal adhesion and microfilament-associated protein VASP EMBO J. 14 1995 19 27
8. M. Halbrugge, C. Friedrich, M. Eigenthaler, P. Schanzenbacher, and U. Walter Stoichiometric and reversible phosphorylation of a 46-kDa protein in human platelets in response to cGMP- and cAMP-elevating vasodilators J. Biol. Chem. 265 1990 3088 3093
9. C. Hanus, C. Vannier, and A. Triller Intracellular association of glycine receptor with gephyrin increases its plasma membrane accumulation rate J. Neurosci. 24 2004 1119 1128
10. K. Harvey, I.C. Duguid, M.J. Alldred, S.E. Beatty, H. Ward, N.H. Keep, S.E. Lingenfelter, B.R. Pearce, J. Lundgren, M.J. Owen, T.G. Smart, B. Luscher, M.I. Rees, and R.J. Harvey The GDP-GTP exchange factor collybistin: an essential determinant of neuronal gephyrin clustering J. Neurosci. 24 2004 5816 5826
11. Q. Jiang, Z. Gu, G. Zhang, and G. Jing Diphosphorylation and involvement of extracellular signal-regulated kinases (ERK1/2) in glutamate-induced apoptotic-like death in cultured rat cortical neurons Brain Res. 857 2000 71 77
12. S. Kins, H. Betz, and J. Kirsch Collybistin, a newly identified brain-specific GEF, induces submembrane clustering of gephyrin Nat. Neurosci. 3 2000 22 29
13. J. Kirsch, and H. Betz The postsynaptic localization of the glycine receptor-associated protein gephyrin is regulated by the cytoskeleton J. Neurosci. 15 1995 4148 4156
14. J. Kirsch, D. Langosch, P. Prior, U.Z. Littauer, B. Schmitt, and H. Betz The 93-kDa glycine receptor-associated protein binds to tubulin J. Biol. Chem. 266 1991 22242 22245
15. J. Kirsch, J. Kuhse, and H. Betz Targeting of glycine receptor subunits to gephyrin-rich domains in transfected human embryonic kidney cells Mol. Cell. Neurosci. 6 1995 450 461
16. M. Kneussel, and H. Betz Clustering of inhibitory neurotransmitter receptors at developing postsynaptic sites: the membrane activation model Trends Neurosci. 23 2000 429 435
17. M. Kneussel, A. Hermann, J. Kirsch, and H. Betz Hydrophobic interactions mediate binding of the glycine receptor beta-subunit to gephyrin J. Neurochem. 72 1999 1323 1326
18. P. Koulen, J.H. Brandstatter, R. Enz, J. Bormann, and H. Wassle Synaptic clustering of GABA(C) receptor rho-subunits in the rat retina Eur. J. Neurosci. 10 1998 115 127
19. M. Krause, A.S. Sechi, M. Konradt, D. Monner, F.B. Gertler, and J. Wehland Fyn-binding protein (Fyb)/SLP-76-associated protein (SLAP), Ena/vasodilator-stimulated phosphoprotein (VASP) proteins and the Arp2/3 complex link T cell receptor (TCR) signaling to the actin cytoskeleton J. Cell Biol. 149 2000 181 194
20. A. Mammoto, T. Sasaki, T. Asakura, I. Hotta, H. Imamura, K. Takahashi, Y. Matsuura, T. Shirao, and Y. Takai Interactions of drebrin and gephyrin with profilin Biochem. Biophys. Res. Commun. 243 1998 86 89
21. G. Meyer, J. Kirsch, H. Betz, and D. Langosch Identification of a gephyrin binding motif on the glycine receptor beta subunit Neuron 15 1995 563 572
22. K. Mortensen, and L.I. Larsson Effects of cytochalasin D on the actin cytoskeleton: association of neoformed actin aggregates with proteins involved in signaling and endocytosis Cell. Mol. Life Sci. 60 2003 1007 1012
23. S.J. Moss, and T.G. Smart Constructing inhibitory synapses Nat. Rev., Neurosci. 2 2001 240 250
24. H. Neuhoff, M. Sassoe-Pognetto, P. Panzanelli, C. Maas, W. Witke, and M. Kneussel The actin-binding protein profilin I is localized at synaptic sites in an activity-regulated manner Eur. J. Neurosci. 21 2005 15 25
25. F. Pfeiffer, R. Simler, G. Grenningloh, and H. Betz Monoclonal antibodies and peptide mapping reveal structural similarities between the subunits of the glycine receptor of rat spinal cord Proc. Natl. Acad. Sci. U. S. A. 81 1984 7224 7227
26. P. Prior, B. Schmitt, G. Grenningloh, I. Pribilla, G. Multhaup, K. Beyreuther, Y. Maulet, P. Werner, D. Langosch, and J. Kirsch Primary structure and alternative splice variants of gephyrin, a putative glycine receptor-tubulin linker protein Neuron 8 1992 1161 1170
27. M. Reinhard, K. Giehl, K. Abel, C. Haffner, T. Jarchau, V. Hoppe, B.M. Jockusch, and U. Walter The proline-rich focal adhesion and microfilament protein VASP is a ligand for profilins EMBO J. 14 1995 1583 1589
28. G. Schwarz, J. Schulze, F. Bittner, T. Eilers, J. Kuper, G. Bollmann, A. Nerlich, H. Brinkmann, and R.R. Mendel The molybdenum cofactor biosynthetic protein Cnx1 complements molybdate-repairable mutants, transfers molybdenum to the metal binding pterin, and is associated with the cytoskeleton Plant Cell 12 2000 2455 2472
29. G. Schwarz, N. Schrader, R.R. Mendel, H.J. Hecht, and H. Schindelin Crystal structures of human gephyrin and plant Cnx1 G domains: comparative analysis and functional implications J. Mol. Biol. 312 2001 405 418
30. M. Sheng Molecular organization of the postsynaptic specialization Proc. Natl. Acad. Sci. U. S. A. 98 2001 7058 7061
31. M. Sola, M. Kneussel, I.S. Heck, H. Betz, and W. Weissenhorn X-ray crystal structure of the trimeric N-terminal domain of gephyrin J. Biol. Chem. 276 2001 25294 25301
32. M. Sola, V.N. Bavro, J. Timmins, T. Franz, S. Ricard-Blum, G. Schoehn, R.W. Ruigrok, I. Paarmann, T. Saiyed, G.A. O'Sullivan, B. Schmitt, H. Betz, and W. Weissenhorn Structural basis of dynamic glycine receptor clustering by gephyrin EMBO J. 23 2004 2510 2519
33. J.D. Sutherland, and M. Way Looking over the edge: a new role for Ena/VASP proteins in lamellipodial dynamics Dev. Cell 2 2002 692 694
34. B. van Zundert, F.J. Alvarez, J.C. Tapia, H.H. Yeh, E. Diaz, and L.G. Aguayo Developmental-dependent action of microtubule depolymerization on the function and structure of synaptic glycine receptor clusters in spinal neurons J. Neurophysiol. 91 2004 1036 1049
35. A. Witteck, Y. Yao, M. Fechir, U. Forstermann, and H. Kleinert Rho protein-mediated changes in the structure of the actin cytoskeleton regulate human inducible NO synthase gene expression Exp. Cell Res. 287 2003 106 115