Collybistin, a newly identified brain-specific GEF, induces submembrane clustering of gephyrin

Nature Neuroscience

Volumn 3, Issue 1, 2000, Pages 22-29

  Kins, Stefan ^a,b   Betz, Heinrich ^a   Kirsch, Joachim ^a,c  

^a MAX PLANCK INSTITUTE FOR BRAIN RESEARCH   (Germany)

^b UNIVERSITY HOSPITAL OF PSYCHIATRY   (Switzerland)

^c UNIVERSITY OF ULM   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

4 AMINOBUTYRIC ACID A RECEPTOR; BRAIN PROTEIN; COLLYBISTIN; GEPHYRIN; GLYCINE RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATASE; RAC PROTEIN; RHO FACTOR; UNCLASSIFIED DRUG;

ARTICLE; EMBRYO; ENZYME ACTIVATION; GENE CLUSTER; GENE CONTROL; HUMAN; HUMAN CELL; INHIBITORY POSTSYNAPTIC POTENTIAL; NERVE CELL PLASTICITY; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN EXPRESSION; PROTEIN INDUCTION; PROTEIN LOCALIZATION;

ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BRAIN; CARRIER PROTEINS; CELL LINE; CONSERVED SEQUENCE; GTP PHOSPHOHYDROLASES; GUANINE NUCLEOTIDE EXCHANGE FACTORS; HUMANS; MACROMOLECULAR SUBSTANCES; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; ORGAN SPECIFICITY; PROTEIN ISOFORMS; RATS; RECEPTORS, GLYCINE; SEQUENCE HOMOLOGY, AMINO ACID; SRC HOMOLOGY DOMAINS; SYNAPSES; SYNAPTIC MEMBRANES;

EID: 0033994755     PISSN: 10976256     EISSN: None     Source Type: Journal    
DOI: 10.1038/71096     Document Type: Article

References (50)

1. Froehner, S. C. Regulation of ion channel distribution at synapses. Annu. Rev. Neurosci. 16, 347-368 (1993).
2. Ehlers, M. D., Mammen, A. L, Lau, L.-F. & Huganir, R. L. Synaptic targeting of glutamate receptors. Curr. Opin. Cell Biol. 8, 484-489 (1996).
3. Kirsch, J., Meyer, G. & Betz, H. Synaptic targeting of ionotropic neurotransmitter receptors. Mol. Cell. Neurosci. 8, 93-98 (1996).
4. Kuhse, J., Betz, H. & Kirsch, J. The inhibitory glycine receptor: architecture, synaptic localization and molecular pathology of a postsynaptic ion-channel complex. Curr. Opin. Neurobiol. 5, 318-323 (1995).
5. Kirsch, J. & Betz, H. Glycine receptor activation is required for receptor clustering in spinal neurons. Nature 392, 717-720 (1998).
6. Lévi, S., Vannier, C. & Triller, A. Strychnine-sensitive stabilization of postsynaptic glycine receptor clusters. J. Cell Sci. 111, 335-345 (1998).
7. Kirsch, J., Wolters, I., Triller, A. & Betz, H. Gephyrin antisense oligonucleotides prevent glycine receptor clustering in spinal neurons. Nature 366, 745-748 (1993).
8. Feng, G. et al. Dual requirement for gephyrin in glycine receptor clustering and molybdoenzyme activity. Science 282, 1321-1324 (1998).
9. A-receptor subunits in the rat retina. J. Comp. Neurol. 357, 1-14 (1995).
10. Todd, A. J., Watt, C., Spike, R. C. & Sieghart, W. Colocalization of GABA, glycine and their receptors at synapses in the rat spinal cord. J. Neurosci. 16, 974-982 (1996).
11. A receptor subtypes requires the γ2 subunit and gephyrin. Nat. Neurosci. 1, 563-571 (1998).
12. A receptor clustering in gephyrin-deficient mice. J. Neurosci. 19, 9289-9297 (1999).
13. Stallmeyer, B. et al. The neurotransmitter-anchoring protein gephyrin reconstitutes molybdenum-cofactor biosynthesis in bacteria, plants and mammalian cells. Proc. Natl. Acad. Sci. USA 96, 1333-1338 (1999).
14. Kirsch, J. et al. The 93-kDa glycine receptor-associated protein binds to tubulin. J. Biol. Chem. 266, 22242-22245 (1991).
15. Kirsch, J. & Betz, H. The postsynaptic localization of the glycine receptor-associated protein gephyrin is regulated by the cytoskeleton. J. Neurosci. 15, 4148-4156 (1995).
16. Kirsch, J., Kuhse, J. & Betz, H. Targeting of glycine receptor subunits to gephyrin-rich domains in transfected human embryonic kidney cells. Mol. Cell. Neurosci. 6, 450-461 (1995).
17. Meyer, G., Kirsch, J., Betz, H. & Langosch, D. Identification of a gephyrin binding motif on the glycine receptor β subunit. Neuron 15, 563-572 (1995).
18. Kneussel, M., Hermann, A., Kirsch, J. & Betz, H. Hydrophobic interactions mediate binding of the glycine receptor β-subunit to gephyrin. J. Neurochem. 72, 1323-1326 (1999).
19. Fields, S. & Song, O. K. A novel genetic system to detect protein-protein interactions. Nature 340, 245-246 (1988).
20. Akagi, H. & Miledi, R. Heterogeneity of glycine receptors and their messenger RNAs in rat brain and spinal cord. Science 242, 270-272 (1988).
21. Hart, M. J. et al. Cellular transformation and guanine nucleotide exchange activity are catalyzed by a common domain on the DBL oncogene product. J. Biol. Chem. 269, 62-65 (1994).
22. Cerione, R. A. & Zheng, Y. The Dbl family of oncogenes. Curr. Opin. Cell Biol. 8, 216-222 (1996).
23. Harlan, J. E., Hajduk, P., Sup Yoon, H. & Fesik, S. W. Pleckstrin homology domains bind to phosphatidylinositol 4,5-bisphosphate. Nature 372, 375-379 (1994).
24. Lim, W. A., Richards, F. M. & Fox, R. O. Structural determinants of peptidebinding orientation and of sequence specificity in SH3 domains. Nature 372, 375-379 (1994).
25. Manser, E. et al. PAK kinases are directly coupled to the PIX family of nucleotide exchange factors. Mol. Cell 1, 183-192 (1998).
26. Pfeiffer, F., Simler, R., Grenningloh, G. & Betz, H. Monoclonal antibodies and peptide mapping reveal structural similarities between the subunits of the glycine receptor of rat spinal cord. Proc. Natl. Acad. Sci. USA 81, 7224-7227 (1984).
27. Kins, S., Kuhse, J., Laube, B., Betz, H. & Kirsch, J. Incorporation of a gephyrin-binding motif targets NMDA receptors to gephyrin-rich domains in HEK 293 cells. Eur. J. Neurosci. 11, 740-744 (1999).
28. Hateboer, G. et al. BS69, a novel adenovirus E1A-associated protein that inhibits E1A transactivation. EMBO J. 14, 3159-3169 (1995).
29. Keino-Masu, K. et al. Deleted in colorectal cancer (DCC) encodes a netrin receptor. Cell 87, 175-185 (1996).
30. Béchade, C., Colin, I., Kirsch, J., Betz, H. & Triller, A. Glycine receptor α subunit and gephyrin expression in cultured spinal neurons: a quantitative analysis. Eur. J. Neurosci. 8, 429-435 (1996).
31. Mammoto, A. et al. Interactions of drebrin and gephyrin with profilin. Biochem. Biophys. Res. Comm. 243, 86-89 (1998).
32. Sabatini, D. et al. RAFT1 signaling requires interaction with the clustering protein gephyrin. Science 284, 1161-1164 (1999).
33. Musacchio, A., Gibson, T., Rice, P., Thompson, J. & Saraste, M. The PH domain: a common piece in the structural patchwork of signalling proteins. Trends Biochem. Sci. 18, 343-348 (1993).
34. Kirsch, J. Assembly of signaling machinery at the postsynaptic membrane. Curr. Opin. Neurobiol. 9, 329-335 (1999).
35. Hall, A. Rho GTPases and the actin cytoskeleton. Science 279, 509-514 (1998).
36. Tapon, N. & Hall, A. Rho, Rac and Cdc42 GTPases regulate the organization of the actin cytoskeleton. Curr. Opin. Cell Biol. 9, 86-92 (1997).
37. Minden, A., Lin, A., Claret, F. X., Abo, A. & Karin, M. Selective activation of the JNK signaling cascade and c-Jun transcriptional activity by the small GTPases Rac and Cdc42Hs. Cell 81, 1147-1157 (1995).
38. Nobes, C. & Hall, A. Rho, Rac and Cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia and filopodia. Cell 81, 53-62 (1995).
39. Chen, H.-J., Rojas-Soto, M., Oguni, A. & Kennedy, M. B. A synaptic ras-GTPase activating protein (p135/SynGAP) inhibited by CaM kinase II. Neuron 20, 895-904 (1998).
40. Kim, J. H., Liao, D., Lau, L.-F. & Huganir, R. L. SynGAP: a synaptic RasGAP that associates with the PSD-95/SAP90 protein family. Neuron 20, 683-691 (1998).
41. Brambilla, R. et al. A role for the ras-signalling pathway in synaptic transmission and longterm memory. Nature 390, 281-286 (1997).
42. Taleb, O. & Betz, H. Expression of the human glycine receptor α1 subunit in Xenopus oocytes: apparent affinities of agonists increase at high receptor densities. EMBO J. 13, 1318-1324 (1994).
43. Harper, J. W., Adami, G. R., Wei, N., Keyomarsi, K. & Elledge, S. J. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75, 805-816 (1993).
44. Prior, P. et al. Primary structure and alternative splice variants of gephyrin, a putative glycine receptor-tubulin linker protein. Neuron 8, 1161-1170 (1992).
45. Frohmann, M. A., Dush, M. K. & Martin, G. R. Rapid production of fulllength cDNAs from rare transcripts: amplification using a single genespecific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85, 8998-9002 (1988).
46. Kirsch, J. & Betz, H. Widespread expression of gephyrin, a putative receptor-tubulin linker protein, in rat brain. Brain Res. 621, 301-310 (1993).
47. Grenningloh, G. et al. The strychnine-binding subunit of the glycine receptor shows homology with nicotinic acetylcholine receptors. Nature 328, 215-220 (1987).
48. Grenningloh, G. et al. Cloning and expression of the 58 kd β subunit of the inhibitory glycine receptor. Neuron 4, 963-970 (1990).
49. Kirsch, J., Malosio, M.-L., Wolters, I. & Betz, H. Distribution of gephyrin transcripts in the adult and developing rat brain. Eur. J. Neurosci. 5, 1109-1117 (1993).
50. Heng, H. H. Q., Squire, J. & Tsui, L.-C. High resolution mapping of mammalian genes by in situ hybridization to free chromatin. Proc. Natl. Acad. Sci. USA 89, 9509-9513 (1992).