Application to immunoassays of the fusion protein between protein ZZ and enhanced green fluorescent protein

Journal of Immunological Methods

Volumn 309, Issue 1-2, 2006, Pages 130-138

  Huang, Qi Lai ^a   Chen, Cheng ^a   Chen, Yun Zi ^a   Gong, Chen Guang ^a   Cao, Lin ^a   Wang, Jin ^a   Hua, Zi Chun ^a  

^a NANJING UNIVERSITY   (China)

Author keywords

Dot blotting; EGFP; Fusion; Immunofluorescent microscopy assay; Protein ZZ

Indexed keywords

BETA3 INTEGRIN; CHIMERIC PROTEIN; GLUTATHIONE TRANSFERASE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; IMMUNOGLOBULIN FC FRAGMENT; INTERFERON REGULATORY FACTOR 3; NICKEL COMPLEX; PROTEIN A; TUMOR NECROSIS FACTOR ALPHA; UNCLASSIFIED DRUG; ZZ PROTEIN;

AFFINITY CHROMATOGRAPHY; ANTIBODY DETECTION; ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; GENETIC TRANSFECTION; IMMUNOASSAY; IMMUNOBLOTTING; IMMUNOFLUORESCENCE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; VIRUS INFECTION; WESTERN BLOTTING;

AMINO ACID SEQUENCE; BASE SEQUENCE; BLOTTING, WESTERN; CELL LINE; CELL SEPARATION; DNA, RECOMBINANT; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; HUMANS; IMMUNOASSAY; IMMUNOBLOTTING; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; LIGANDS; MICROSCOPY, FLUORESCENCE; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; TUMOR NECROSIS FACTOR-ALPHA;

AEQUOREA VICTORIA; ANIMALIA; ESCHERICHIA COLI;

EID: 31944433101     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2005.11.009     Document Type: Article

References (43)

1. C. Akgul, D.A. Moulding, M.R.H. White, and S.W. Edwards In vivo localisation and stability of human Mcl-1 using green fluorescent protein (GFP) fusion proteins FEBS Lett. 478 2000 72
2. T. Aoki, Y. Takahashi, K.S. Koch, H.L. Leffert, and H. Watabe Construction of a fusion protein between protein A and green fluorescent protein and its application to Western blotting FEBS Lett. 384 1996 193
3. R. Arai, H. Ueda, and T. Nagamune Construction of chimeric proteins between protein G and fluorescence-enhanced green fluorescent protein, and their application to immunoassays J. Ferment. Bioeng. 86 1998 440
4. W.C. Au, and P.M. Pitha Recruitment of multiple interferon regulatory factors and histone acetyltransferase to the transcriptionally active interferon a promoters J. Biol. Chem. 276 2001 41629
5. W.-C. Au, P.A. Moore, W. Lowther, Y.-T. Juang, and P.M. Pitha Identification of a member of the interferon regulatory factor family that binds to the Interferon-stimulated response element and activates expression of Interferon-induced genes Proc. Natl. Acad. Sci. U. S. A. 92 1995 11657
6. J.L. Casey, A.M. Coley, L.M. Tilley, and M. Foley Green fluorescent antibodies: novel in vitro tools Protein Eng. 13 2000 445
7. M. Chalfie, Y. Tu, G. Euskirchen, W.W. Ward, and D.C. Prasher Green fluorescent protein as a marker for gene expression Science 263 1994 802
8. D.A. Cheresh Human endothelial cells synthesize and express an Arg-Gly-Asp directed adhesion receptor involved in attachment to fibrinogen and von Willebrand factor Proc. Natl. Acad. Sci. U. S. A. 84 1987 6471
9. C.W. Cody, D.C. Prasher, W.M. Westler, F.G. Prendergast, and W.W. Ward Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein Biochemistry 32 1993 1212
10. B.P. Cormack, R.H. Valdivia, and S. Falkow FACS-optimized mutants of the green fluorescent protein (GFP) Gene 173 1996 33
11. K.A. Fitzgerald, S.M. McWhirter, K.L. Faia, D.C. Rowe, E. Latz, D.T. Golenbock, A.J. Coyle, S.M. Liao, and T. Maniatis IKKε and TBK1 are essential components of the IRF3 signaling pathway Nat. Immun. 4 2003 491
12. R.A. Griep, C. van Twisk, J.M. van der Wolf, and A. Schots Fluobodies: green fluorescent single-chain Fv fusion proteins J. Immunol. Methods 230 1999 121
13. J. Haas, E.C. Park, and B. Seed Codon usage limitation in the expression of HIV-1 envelope glycoprotein Curr. Biol. 6 1996 315
14. R. Heim, D.C. Prasher, and R.Y. Tsien Wavelength mutations and posttranslational autoxidation of green fluorescent protein Proc. Natl. Acad. Sci. U. S. A. 91 1994 12501
15. M.A. Hink, R.A. Griep, J.W. Borst, A. van Hoek, M.H.M. Eppink, A. Schots, and A.J.W.G. Visser Structural dynamics of green fluorescent protein alone and fused with a single chain Fv protein J. Biol. Chem. 275 2000 17556
16. Z. Hua, L. Jie, and D. Zhu Expression of a fibrinolytically active human pro-urokinase fusion protein in Escherichia coli Biochem. Mol. Biol. Int. 33 1994 1215
17. Z. Hua, L. Jie, and D. Zhu Expression of a biologically active human granulocyte-macrophage colony stimulating factor fusion protein in Escherichia coli Biochem. Mol. Biol. Int. 34 1994 621
18. O.A. Koroleva, M.L. Tomlinson, D. Leader, P. Shaw, and J.H. Doonan High-throughput protein localization in Arabidopsis using Agrobacterium-mediated transient expression of GFP-ORF fusions Plant J. 41 2005 162
19. P.I. Leavesley, G.D. Ferguson, E.A. Wayner, and D.A. Cheresh Requirement of the integrin β3 subunit for carcinoma cell spreading or migration on vitronectin and fibrinogen J. Cell Biol. 117 1992 1101
20. K. Lobeck, P. Drevet, M. Leonetti, C. Fromen-Romano, F. Ducancel, E. Lajeunesse, C. Lemaire, and A. Menez Towards a recombinant vaccine against diphtheria toxin Infect. Immun. 66 1998 418
21. B. Lowenadler, B. Jansson, S. Paleus, E. Holmgren, B. Nilsson, T. Moks, G. Palm, S. Josephson, L. Philipson, and M. Uhlen A gene fusion system for generating antibodies against short peptides Gene 58 1987 87
22. F.J.M. Mergulhão, M.A. Taipa, J.M.S. Cabral, and G.A. Monteiro Evaluation of bottlenecks in proinsulin secretion by Escherichia coli J. Biotechnol. 109 2004 31
23. K. Morino, H. Katsumi, Y. Akahori, Y. Iba, M. Shinohara, Y. Ukai, Y. Kohara, and Y. Kurosawa Antibody fusions with fluorescent proteins: a versatile reagent for profiling protein expression J. Immunol. Methods 257 2001 175
24. Y. Nagai, M. Miyazaki, R. Aoki, T. Zama, S. Inouye, K. Hirose, M. Iino, and M. Hagiwara A fluorescent indicator for visualizing cAMP-induced phosphorylation in vivo Nat. Biotechnol. 18 2000 313
25. M. Nakayama, and O. Ohara A system using convertible vectors for screening soluble recombinant proteins produced in Escherichia coli from randomly fragmented cDNAs Biochem. Biophys. Res. Commun. 312 2003 825
26. B. Nilsson, T. Moks, B. Jansson, L. Abrahmsen, A. Elmblad, E. Holmgren, C. Henrichson, T.A. Jones, and M. Uhlen A synthetic IgG-binding domain based on staphylococcal protein A Protein Eng. 1 1987 107
27. K. Omoya, Z. Kato, E. Matsukuma, A.L. Li, K. Hashimoto, Y. Yamamoto, H. Ohnishi, and N. Kondo Systematic optimization of active protein expression using GFP as a folding reporter Protein Expr. Purif. 36 2004 327
28. J.J. Oprandy, J.G. Olson, and T.W. Scott A rapid dot immunoassay for the detection of serum antibodies to Eastern equine encephalomyelitis and St. Louis encephalitis viruses in sentinel chickens Am. J. Trop. Med. Hyg. 38 1988 181
29. M. Ormö, A. Cubitt, K. Kallio, L. Gross, R. Tsien, and S. Remington Crystal structure of the Aequorea victoria green fluorescent protein Science 273 1996 1392
30. M. Peipp, D. Saul, K. Barbin, J. Bruenke, S.J. Zunino, M. Niederweis, and G.H. Fey Efficient eukaryotic expression of fluorescent scFv fusion proteins directed against CD antigens for FACS applications J. Immunol. Methods 285 2004 265
31. D.C. Prasher, V.K. Eckenrode, W.W. Ward, F.G. Prendergast, and M.J. Cormier Primary structure of the Aequorea victoria green fluorescent protein Gene 111 1992 229
32. R. Rizzuto, M. Brini, P. Pizzo, M. Murgia, and T. Pozzan Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells Curr. Biol. 5 1995 635
33. H. Rondahl, B. Nilsson, and E. Holmgren Fusions to the 5′ end of a gene encoding a two-domain analogue of staphylococcal protein A J. Biotechnol. 25 1992 269
34. E. Ruoslahti RGD and other recognition sequences for integrins Annu. Rev. Cell Dev. Biol. 12 1996 697
35. G. Schwalbach, A.P. Sibler, L. Choulier, F. Deryckere, and E. Weiss Production of fluorescent single-chain antibody fragments in Escherichia coli Protein Expr. Purif. 18 2000 121
36. S. Sharma, B.R. ten Oever, N. Grandvaux, G.P. Zhou, R. Lin, and J. Hiscott Triggering the interferon antiviral response through an IKK-related pathway Science 300 2003 1148
37. O. Shimomura Structure of the chromophore of Aequorea green fluorescent protein FEBS Lett. 104 1979 220
38. O. Shimomura, F.H. Johnson, and Y. Saiga Extraction, purification and properties of aequorin, a bioluminescent protein from luminous hydromedusan, Aequorea J. Cell. Comp. Physiol. 59 1962 223
39. M.G. Wathelet, C.H. Lin, B.S. Parekh, L.V. Ronco, P.M. Howley, and T. Maniatis Virus infection induces the assembly of coordinately activated transcription factors on the IFN-beta enhancer in vivo Mol. Cell 1 1998 507
40. F.S. Wouters, P.J. Verveer, and P.I.H. Bastiaens Imaging biochemistry inside cells Trends Cell Biol. 11 2001 203
41. W. Xie, Q. Qiu, H. Wu, and X. Xu Expression of cecropin CMIV fusion protein in E. coli under T7 promoter Biochem. Mol. Biol. Int. 39 1996 487
42. Y. Yan, and G. Marriott Analysis of protein interactions using fluorescence technologies Curr. Opin. Chem. Biol. 7 2003 635
43. F. Yang, L.G. Moss, and G.N. Phillips Jr. The molecular structure of green fluorescent protein Nat. Biotechnol. 14 1996 1246