Identification of a virulence-associated determinant, dihydrolipoamide dehydrogenase (lpd), in Mycoplasma gallisepticum through in vivo screening of transposon mutants

Infection and Immunity

Volumn 74, Issue 2, 2006, Pages 931-939

  Hudson, P ^a   Gorton, T S ^a   Papazisi, L ^a,b   Cecchini, K ^a   Frasca Jr , S ^a   Geary, S J ^a  

^a UNIVERSITY OF CONNECTICUT   (United States)

^b J CRAIG VENTER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIHYDROLIPOAMIDE DEHYDROGENASE; PRIMER DNA;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BACTERIAL GENE; BACTERIAL GENOME; BACTERIAL SURVIVAL; BACTERIAL VIRULENCE; BACTERIUM; BACTERIUM TRANSFORMATION; CHICKEN; DNA FLANKING REGION; DNA SYNTHESIS; GENE INSERTION; GENE SEQUENCE; GENETIC CODE; LPD GENE; MUTAGENESIS; MYCOPLASMA GALLISEPTICUM; NONHUMAN; PRIORITY JOURNAL; RESPIRATORY SYSTEM; SCREENING; SEQUENCE ANALYSIS; SIGNATURE SEQUENCE MUTAGENESIS; TRANSPOSON;

ANIMALS; BACTERIAL PROTEINS; CHICKENS; DIHYDROLIPOAMIDE DEHYDROGENASE; DNA TRANSPOSABLE ELEMENTS; MUTAGENESIS, INSERTIONAL; MYCOPLASMA GALLISEPTICUM; MYCOPLASMA INFECTIONS; POULTRY DISEASES; SEQUENCE ANALYSIS, DNA; SPECIFIC PATHOGEN-FREE ORGANISMS; VIRULENCE;

EID: 31844440317     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.74.2.931-939.2006     Document Type: Article

References (41)

1. Altwood, T. K. 2002. The PRINTS database: a resource for identification of protein families. Brief Bioinform. 3:252-263.
2. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
3. Bateman, A., E. Birney, L. Cerruti, R. Durbin, L. Etwiller, S. R. Eddy, S. Griffiths-Jones, K. L. Howe, M. Marshall, and E. L. Sonnhammer. 2002. The Pfam protein families database. Nucleic Acids Res. 30:276-280.
4. Benen, J., W. van Berkel, C. Veeger, and A. de Kok. 1992. Lipoamide dehydrogenase from Azotobacter vinelandii. The role of the C-terminus in catalysis and dimer stabilization. Eur. J. Biochem. 207:499-505.
5. Byrne, M. E., D. A. Rouch, and R. A. Skurray. 1989. Nucleotide sequence analysis of IS256 from the Staphylococcus aureus gentamicin-tobramycin- kanamycin-resistance transposon Tn4001. Gene 81:361-367.
6. Cao, J., P. A. Kapke, and F. C. Minion. 1994. Transformation of Mycoplasma gallisepticum with Tn916, Tn4001, and integrative plasmid vectors. J. Bacteriol. 176:4459-4462.
7. Chambaud, I., R. Heilig, S. Ferris, V. Barbe, D. Samson, F. Galisson, I. Moszer, K. Dybvig, H. Wroblewski, A. Viari, E. P. Rocha, and A. Blanchard. 2001. The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis. Nucleic Acids Res. 29:2145-2153.
8. Chanock, R. M., L. Hayflick, and M. F. Barile. 1962. Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO. Proc. Natl. Acad. Sci. USA 48:41-49.
9. Constantopoulos, G., and G. J. McGarrity. 1987. Activities of oxidative enzymes in mycoplasmas. J. Bacteriol. 169:2012-2016.
10. Dybvig, K., and L. L. Voelker. 1996. Molecular biology of mycoplasmas. Annu. Rev. Microbiol. 50:25-57.
11. Falquet, L., M. Pagni, P. Bucher, N. Hulo, C. J. Sigrist, K. Hofmann, and A. Bairoch. 2002. The PROSITE database, its status in 2002. Nucleic Acids Res. 30:235-238.
12. Foissac, X., C. Saillard, and J. M. Bove. 1997. Random insertion of transposon Tn4001 in the genome of Spiroplasma citri strain GII3. Plasmid 37:80-86.
13. Fraser, C. M., J. D. Gocayne, O. White, M. D. Adams, R. A. Clayton, R. D. Fleischmann, C. J. Bult, A. R. Kerlavage, G. Sutton, J. M. Kelley, et al. 1995. Tbe minimal gene complement of Mycoplasma genitalium. Science 270:397-403.
14. Hedreyda, C. T., K. K. Lee, and D. C. Krause. 1993. Transformation of Mycoplasma pneumoniae with Tn4001 by electroporation. Plasmid 30:170-175.
15. Heiner, C. R., K. L. Hunkapiller, S. M. Chen, J. I. Glass, and E. Y. Chen. 1998. Sequencing multimegabase-template DNA with BigDye terminator chemistry. Genome Res. 8:557-561.
16. Henikoff, J. G., E. A. Greene, S. Pietrokovski, and S. Henikoff. 2000. Increased coverage of protein families with the blocks database servers. Nucleic Acids Res. 28:228-230.
17. Hensel, M., J. E. Shea, C. Gleeson, M. D. Jones, E. Dalton, and D. W. Holden. 1995. Simultaneous identification of bacterial virulence genes by negative selection. Science 269:400-403.
18. Herbert, M., A. Kraiss, A. K. Hilpert, S. Schlor, and J. Reidl. 2003. Aerobic growth deficient Haemophilus influenzae mutants are non-virulent: implications on metabolism. Int. J. Med. Microbiol. 293:145-152.
19. Heungens, K., C. E. Cowles, and H. Goodrich-Blair. 2002. Identification of Xenorhabdus nematophila genes required for mutualistic colonization of Steinernema carpocapsae nematodes. Mol. Microhiol. 45:1337-1353.
20. Himmelreich, R., H. Hilbert, H. Plagens, E. Pirkl, B. C. Li, and R. Herrmann. 1996. Complete sequence analysis of the genome of the bacterium Mycoplasma pneumonias. Nucleic Acids Res. 24:4420-4449.
21. Hutchison, C. A., S. N. Peterson, S. R. Gill, R. T. Cline, O. White, C. M. Fraser, H. O. Smith, and J. C. Venter. 1999. Global transposon mutagenesis and a minimal Mycoplasma genome. Science 286:2165-2169.
22. Jones, D. T. 1999. Protein secondary structure prediction based on position-specific scoring matrices. J. Mol. Biol. 292:195-202.
23. Jones, D. T., W. R. Taylor, and J. M. Thornton. 1994. A model recognition approach to the prediction of all-helical membrane protein structure and topology. Biochemistry 33:3038-3049.
24. Jordan, F. T. W. 1979. Avian mycoplasmas, p. 9-16. In J. C. Tully and R. F. Whitcomb (ed.), The mycoplasmas, vol. 2. Academic Press, New York, N.Y.
25. Knudtson, K. L., and F. C. Minion. 1993. Construction of Tn4001lac derivatives to be used as promoter probe vectors in mycoplasmas. Gene 137:217-222.
26. Krogh, A., B. Larsson, G. von Heijne, and E. L. Sonnhammer. 2001. Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J. Mol. Biol. 305:567-580.
27. Li de la Sierra, I., L. Pernot, T. Prange, P. Saludjian, M. Schiltz, R. Fourme, and G. Padron. 1997. Molecular structure of the lipoamide dehydrogenase domain of a surface antigen from Neisseria meningitidis. J. Mol. Biol. 269:129-141.
28. Matic, J. N., J. L. Wilton, R. J. Towers, A. L. Scarman, F. C. Minion, M. J. Walker, and S. P. Djordjevic. 2003. The pyruvate dehydrogenase complex of Mycoplasma hyopneumoniae contains a novel lipoyl domain arrangement. Gene 319:99-106.
29. Mattevi, A., G. Obmolova, K. H. Kalk, W. J. van Berkel, and W. G. Hol. 1993. Three-dimensional structure of lipoamide dehydrogenase from Pseudomonas fluorescens at 2.8 A resolution. Analysis of redox and thermostability properties. J. Mol. Biol. 230:1200-1215.
30. McGuffin, L. J., K. Bryson, and D. T. Jones. 2000. The PSIPRED protein structure prediction server. Bioinformatics 16:404-405.
31. Nunoya, T., M. Tajima, T. Yagihashi, and S. Sannai. 1987. Evaluation of respiratory lesions in chickens induced by Mycoplasma gallisepticum. Nippon Juigaku Zasshi 49:621-629.
32. Papazisi, L., S. Frasca, Jr., M. Gladd, X. Liao, D. Yogev, and S. J. Geary. 2002. GapA and CrmA coexpression is essential for Mycoplasma gallisepticum cytadherence and virulence. Infect. Immun. 70:6839-6845.
33. Papazisi, L., T. S. Gorton, G. Kutish, P. F. Markham, G. F. Browning, D. K. Nguyen, S. Swartzell, A. Madan, G. Mahairas, and S. J. Geary. 2003. The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low). Microbiology 149:2307-2316.
34. Papazisi, L., L. K. Silbart, S. Frasca, D. Rood, X. Liao, M. Gladd, M. A. Javed, and S. J. Geary. 2002. A modified live Mycoplasma gallisepticum vaccine to protect chickens from respiratory disease. Vaccine 20:3709-3719.
35. Papazisi, L., K. E. Troy, T. S. Gorton, X. Liao, and S. J. Geary. 2000. Analysis of cytadherence-deficient, GapA-negative Mycoplasma gallisepticum strain R. Infect. Immun. 68:6643-6649.
36. Pollack, J. D., M. V. Williams, and R. N. McElhaney. 1997. The comparative metabolism of the mollicutes (Mycoplasmas): the utility for taxonomic classification and the relationship of putative gene annotation and phylogeny to enzymatic function in the smallest free-living cells. Crit. Rev. Microbiol. 23:269-354.
37. Rost, B., and J. Liu. 2003. The PredictProtein server. Nucleic Acids Res. 31:3300-3304.
38. Sasaki, Y., J. Ishikawa, A. Yamashita, K. Oshima, T. Kenri, K. Furuya, C. Yoshino, A. Horino, T. Shiba, T. Sasaki, and M. Hattori. 2002. The complete genomic sequence of Mycoplasma penetrans, an intracellular bacterial pathogen in humans. Nucleic Acids Res. 30:5293-5300.
39. Sheehan, D. C., and B. B. Hrapchak. 1980. Theory and practice of histotechnology, 2nd ed. Mosby, St. Louis, Mo.
40. Soeripto, K. G. Whithear, G. S. Cottew, and K. E. Harrigan. 1989. Virulence and transmissibility of Mycoplasma gallisepticum. Aust. Vet. J. 66:65-72.
41. Whithear, K. G. 1996. Control of avian mycoplasmoses by vaccination. Rev. Sci. Technol. 15:1527-1553.