Secretory expression and characterization of a recombinant-deleted variant of human hepatocyte growth factor in Pichia pastoris

World Journal of Gastroenterology

Volumn 11, Issue 45, 2005, Pages 7097-7103

  Liu, Zhi Min ^a   Zhao, Hong Liang ^a   Xue, Chong ^a   Deng, Bing Bing ^a   Zhang, Wei ^a   Xiong, Xiang Hua ^a   Yang, Bing Fen ^a   Yao, Xue Qin ^a  

^a BEIJING INSTITUTE OF BIOTECHNOLOGY   (China)

Author keywords

Hepatocyte growth factor; Pichia pastoris; Secretory expressing

Indexed keywords

COMPLEMENTARY DNA; MATING HORMONE ALPHA FACTOR; MESSENGER RNA; RECOMBINANT SCATTER FACTOR;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BIOMASS; BIOTRANSFORMATION; CELL CULTURE; CELL DENSITY; CHEMICAL PROCEDURES; CONTROLLED STUDY; CULTURE TECHNIQUE; ESCHERICHIA COLI; FED BATCH CULTURE; FERMENTATION; FUNGAL STRAIN; GENE INSERTION; GENETIC CODE; GENETIC SCREENING; INDUSTRY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PICHIA PASTORIS; PLACENTA; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRODUCTIVITY; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN SECRETION; RESEARCH; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SHUTTLE VECTOR; SUPERNATANT; WESTERN BLOTTING; YEAST;

EID: 31544444440     PISSN: 10079327     EISSN: None     Source Type: Journal    
DOI: 10.3748/wjg.v11.i45.7097     Document Type: Article

References (27)

1. Kataoka H, Miyata S, Uchinokura S, Itoh H. Roles of hepatocyte growth factor (HGF) activator and HGF activator inhibitor in the pericellular activation of HGF/scatter factor. Cancer Metastasis Rev 2003; 22: 223-236
2. Kinosaki M, Yamaguchi K, Murakami A, Morinaga T, Ueda M, Higashio K. Analysis of deleted variant of hepatocyte growth factor by alanine scanning mutagenesis: identification of residues essential for its biological function and generation of mutants with enhanced mitogenic activity on rat hepatocytes. FEBS Lett 1998; 434: 165-170
3. Weidner KM, Arakaki N, Hartmann G, Vandekerckhove J, Weingart S, Rieder H, Fonatsch C, Tsubouchi H, Hishida T, Daikuhara Y. Evidence for the identity of human scatter factor and human hepatocyte growth factor. Proc Natl Acad Sci U S A 1991; 88: 7001-7005
4. Matsumoto K, Nakamura T. Emerging multipotent aspects of hepatocyte growth factor. J Biochem (Tokyo) 1996; 119: 591-600
5. Godowski PJ, Lokker NA, Mark MR. Single-chain hepatocyte growth factor variants. United States Patent 5580963, 1996
6. Shima N, Tsuda E, Goto M, Yano K, Hayasaka H, Ueda M, Higashio K. Hepatocyte growth factor and its variant with a deletion of five amino acids are distinguishable in their biological activity and tertiary structure. Biochem Biophys Res Commun 1994; 200: 808-815
7. Kinosaki M, Yamaguchi K, Murakami A, Ueda M, Morinaga T, Higashio K. Identification of heparin-binding stretches of a naturally occurring deleted variant of hepatocyte growth factor (dHGF). Biochim Biophys Acta 1998; 1384: 93-102
8. Yasuda H, Imai E, Shiota A, Fujise N, Morinaga T, Higashio K. Antifibrogenic effect of a deletion variant of hepatocyte growth factor on liver fibrosis in rats. Hepatology 1996; 24: 636-642
9. Shiota G, Rhoads DB, Wang TC, Nakamura T, Schmidt EV. Hepatocyte growth factor inhibits growth of hepatocellular carcinoma cells. 1992; 89: 373-377
10. Cregg JM, Vedvick TS, Raschke WC. Recent advances in the expression of foreign genes in Pichia pastoris. Biotechnology (N Y) 1993; 11: 905-910
11. Brierley RA, Bussineau C, Kosson R, Melton A, Siegel RS. Fermentation development of recombinant Pichia pastoris expressing the heterologous gene: bovine lysozyme. Ann NY Acad Sci 1990; 589: 350-362
12. O'Leary JM, Radcliffe CM, Willis AC, Dwek RA, Rudd PM, Downing AK. Identification and removal of O-linked and non-covalently linked sugars from recombinant protein produced using Pichia pastoris. Protein Expr Purif 2004; 38: 217-227
13. Zhang W, Li ZJ, and Foster A Agblevor. Microbubble fermentation of recombinant Pichia pastoris for human serum albumin production. Process Biochemistry 2005; 40: 2073-2078
14. Werten MW, van den Bosch TJ, Wind RD, Mooibroek H, de Wolf FA. High-yield secretion of recombinant gelatins by Pichia pastoris. Yeast 1999; 15: 1087-1096
15. James M Cregg, Joan Lin Cereghino, Jianying Shi, David R Higgins. Recombinant protein expression in Pichia pastoris. Molecular Biotechnology 2000; 16: 23-52
16. Nakamura T, Nishizawa T, Hagiya M, Seki T, Shimonishi M, Sugimura A, Tashiro K, Shimizu S. Molecular cloning and expression of human hepatocyte growth factor. Nature 1989; 342: 440-443
17. Sambrook J and Russell DW. Molecular cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Third Edition, New York, 2001
18. Schagger H, von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 1987; 166: 368-379
19. Sanchez JC, Wirth P, Jaccoud S, Appel RD, Sarto C, Wilkins MR, Hochstrasser DF. Simultaneous analysis of cyclin and oncogene expression using multiple monoclonal antibody immunoblots. Electrophoresis 1997; 18: 638-641
20. Jin H, Wyss JM, Yang R, Schwall R. The therapeutic potential of hepatocyte growth factor for myocardial infarction and heart failure. Curr Pharm Des 2004; 10: 2525-2533
21. Yuan AH and Mei CL. effects of hepatocyte growth factor on synthesis of extracellular matrix and matrix metalloproteinases and tissue inhibitor of metalloproteinases in autosomal dominant polycystic kidney disease cyst-lining epithelial cells. Dier Junyi Daxue Xuebao 2003; 24: 11-17
22. Wu SG, Yu CL, Xu W, Guo YJ, and Ce XY. Expression and biological activity of human hepatocyte growth factor (HGF) by Chinese hamster ovary (CHO) cells. Diyi Junyi Daxue Xuebao 1995; 15: 321-324
23. Qiu YC, Zhu ZG, Xu JH, Xu W, Wu SG. Purification and identification of human recombinant hepatocyte growth factor expressed by CHO cells. Acad J First Mil Med Univ 2001; 21: 332-333
24. Wang MY, Yang YH, Lee HS, Lai SY. Production of functional hepatocyte growth factor (HGF) in insect cells infected with an HGF-recombinant baculovirus in a serum-free medium. Biotechnol Prog 2000; 16: 146-151
25. Bosch A, McCray PB Jr, Walters KS, Bodner M, Jolly DJ, van Es HH, Nakamura T, Matsumoto K, Davidson BL. Effects of keratinocyte and hepatocyte growth factor in vivo: implications for retrovirus-mediated gene transfer to liver. Hum Gene Ther 1998; 9: 1747-1754
26. Dang SY, Cheng NL, Niu B, Wang HZ, Zhao JB. Expression of human recombinant hepatocyte growth factor heavy chain in Escherichia coli. J Shanxi Med Univ 2000; 31: 481-483
27. Li XG, Gu YL, Zhu YS, Song HY. Molecular cloning of cDNA gene encoding human hepatocyte growth factor and its expression with Pichia. Pharmaceutical Biotechnology 1997; 4: 193-197