Production of functional hepatocyte growth factor (HGF) in insect cells infected with an HGF-recombinant baculovirus in a serum-free medium

Biotechnology Progress

Volumn 16, Issue 2, 2000, Pages 146-151

  Wang, Min Ying ^a   Yang, Ya Huey ^a   Lee, Hsuan Shu ^b   Lai, Su Yuan ^b,c  

^a NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

^b NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^c CHUNGTAI INST HLTH SCI TECHNOL   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

CELL GROWTH; CELL LINE; CELL SECRETION; CULTURE MEDIUM; INSECT CELL CULTURE; PRO HEPATOCYTE GROWTH FACTOR; SCATTER FACTOR; SERUM PROTEASE; VIRUS INFECTION;

ANIMAL CELL CULTURE; BIOCONVERSION; BIOSYNTHESIS; ENZYME KINETICS; PHYSIOLOGICAL MODELS; PROTEINS; PURIFICATION; VIRUSES;

BACULOVIRAL INFECTION; HEPATOCYTE GROWTH FACTOR (HGF);

BIOTECHNOLOGY;

ANIMALS; BACULOVIRIDAE; BIOTECHNOLOGY; CELL DIVISION; CELL LINE; CHROMATOGRAPHY, AFFINITY; CULTURE MEDIA, SERUM-FREE; GENETIC VECTORS; HEPARIN; HEPATOCYTE GROWTH FACTOR; PROTEIN ENGINEERING; PROTEIN PRECURSORS; RATS; RECOMBINANT PROTEINS; SPODOPTERA;

ANIMALIA; INSECTA; INVERTEBRATA; SPODOPTERA LITURA; UNIDENTIFIED BACULOVIRUS;

EID: 0034092142     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp990143t     Document Type: Article

References (39)

1. Michalopoulos, G. K.; Houck, K. A.; Dolan, M. L.; Luetteke, N C. Control of hepatocyte proliferation by two serum factors. Cancer Res. 1984, 44, 4414-4419.
2. Nakamura, T.; Nawa, K.; Ichihara, A. Partial purification and characterization of hepatocyte growth factor from serum of hepatectomized rats. Biochem. Biophys. Res. Commun. 1984, 122, 1450-1459.
3. Nakamura, T.; Nawa, K.; Ichihara, A.; Kaise, N.; Nishino, T. Purification and subunit structure of hepatocyte growth factor from rat platelets. FEBS Lett. 1987, 224, 311-316.
4. Gohda, E.; Tsubouchi, H.; Nakayama, H.; Hirono, S.; Sakiyama, O.; Takahashi, K.; Miyazaki, H.; Hashimoto, S.; Daikuhara, Y. Purification and partial characterization of hepatocyte growth factor from plasma of a patient with fulminant hepatic failure. J. Clin. Invest. 1988, 81, 414-419.
5. Zarnegar, R.; Michalopoulos, G. K. Purification and biological characterization of human hepatopoietin A, a polypeptide growth factor for hepatocyte. Cancer Res. 1989, 49, 3314-3320.
6. Kan, M.; Zhang, G. H.; Zarnegar, R.; Michalopoulos, G.; Myoken, Y.; McKeehan, W. L.; Stevens, J. I. Hepatocyte growth factor/hepatopoietin A stimulates the growth of rat kidney proximal tubule epithelial cells (RPTE), rat nonparenchymal liver cells, human melanoma cells, mouse keratinocytes and stimulates anchorage-independent growth of SV-40 transformed RPTE. Biochem. Biophys. Res. Commun. 1991, 174, 331-337.
7. Igawa, T.; Kanda, S.; Kanetake, H.; Saitoh, Y.; Ichihara, A.; Tomita, Y.; Nakamura T. Hepatocyte growth factor is a potent mitogen for cultured rabbit renal tubular epithelial cells. Biochem. Biophys. Res. Commun. 1991, 174, 831-838.
8. Matsumoto, K.; Tajima, H.; Nakamura, T. Hepatocyte growth factor is a potent stimulator of human melanocyte DNA synthesis and growth. Biochem. Biophys. Res. Commun. 1991, 176, 45-51.
9. Rubin, J. S.; Chan, A. M.; Bottaro, D. P.; Burgess, W. H.; Taylor, W. G.; Cech, A. C.; Hirschfield, D. W.; Wong, J.; Miki, T.; Finch, P. W. A broad-spectrum human lung fibroblastderived mitogen is a variant of hepatocyte growth factor. Proc. Natl. Acad. Sci. U.S.A. 1991, 88, 415-419.
10. Grant, D. S.; Kleinman, H. K; Goldberg, I. D.; Bhargava, M. M.; Nickoloff, B. J.; Kinsella, J. L.; Polverini, P.; Rosen, E. M. Scatter factor induces blood vessel formation in vivo. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 1937-1941.
11. Montesano, R.; Matsumoto, K.; Nakamura, T.; Orci, L. Identification of a fibroblast-derived epithelial morphogen as hepatocyte growth factor. Cell 1991, 67, 901-908.
12. Weidner, K. M.; Arakaki, N.; Hartmann, G.; Vandekerckhove, J.; Weingart, S.; Rieder, H.; Fonatsch, C.; Tsubouchi, H.; Hishida, T.; Daikuhara, Y. Evidence for the identity of human scatter factor and human hepatocyte growth factor. Proc. Natl. Acad. Sci. U.S.A. 1991, 88, 7001-7005.
13. Miyazawa, K.; Tsubouchi, H.; Naka, D.; Takahashi, K.; Okigaki, M.; Arakaki, N.;Nakayama, H.; Hirono, S.; Sakiyama, O.; Takahashi, K.; Kitamura, N. Molecular cloning and sequence analysis of cDNA for human hepatocyte growth factor. Biochem. Biophys. Res. Commun. 1989, 163, 967-973.
14. Nakamura, T.; Nishizawa, T.; Hagiya, M.; Seki, T.; Shimonishi, M.; Sugimura, A.; Tashiro, K.; Shimizu, S. Molecular cloning and expression of human hepatocyte growth factor. Nature 1989, 342, 440-443.
15. Miyazawa, K; Kitamura, A.; Naka, D.; Kitamura, N. An alternatively processed mRNA generated from human hepatocyte growth factor gene. Eur. J. Biochem. 1991, 197, 15-22.
16. Hernandez, J. R.; Michalopoulos, G. K. Characterization of the effects of human placental HGF on rat hepatocytes. J. Cell Physiol. 1992, 150, 116-121.
17. Naka, D.; Ishii, T.; Yoshiyama, Y.; Miyazawa, K.; Hara, H.; Hishida, T.; Kitamura, N. Activation of hepatocyte growth factor by proteolytic conversion of a single chain form to a heterodimer. J. Biol. Chem. 1992, 267, 20114-20119.
18. Gak, E.; Taylor, W. G.; Chan, A. M. L.; Rubin, J. S. Processing of hepatocyte growth factor to the heterodimeric form is required for biological activity. FEBS Lett. 1992, 311, 17-21.
19. Hartmann, G.; Naldini, L.; Weidner, K. M.; Sachs, M.; Vigna, E.; Comoglio, P. M.; Birchmeier, W. A functional domain in the heavy chain of scatter factor/hepatocyte growth factor binds the c-Met receptor and induces cell dissociation but not mitogenesis. Proc. Natl. Acad. Sci. U.S.A. 1992, 89, 11574-11578.
20. Lokker, N. A.; Mark, M. R.; Luis, E. A.; Bennett, G. L.; Robbins, K. A.; Baker, J. B.; Godowski, P. J. Structure -function analysis of hepatocyte growth factor: identification of variants of that lack mitogenic activity yet retain high affinity receptor binding. EMBO J. 1992, 11, 2503-2510.
21. Shimomura, T.; Ochiai, M.; Kondo, J.; Morimoto, Y. A novel protease obtained from FBS-containing culture supernatant that processes single chain form hepatocyte growth factor to two chain form in serum-free culture. Cytotechnology 1992, 8, 219-229.
22. Miyazawa, K.; Shimomura, T.; Kitamura. A.; Kondo, J.; Morimoto, Y.; Kitamura, N. Activation of the zymogen of hepatocyte growth factor activator by thrombin. J. Biol. Chem. 1993, 268, 10024-10028.
23. Mizuno, K.; Tanone, Y.; Okano, I.; Harano, T.; Takada, K.; Nakamura, T. Purification and characterization of hepatocyte growth factor (HGF)-converting enzyme: activation of pro-HGF. Biochem. Biophys. Res. Commun. 1994, 198, 1161-1169.
24. Lee, H. S.; Huang, G. T.; Sheu, J. C.; Chiou, L. L.; Lai, M. Y.; Chen, D. S.; Lee, S. C. Characterization of hepatocyte growth factor expressed by baculovirus. Biochem. Biophys. Res. Commun. 1993, 197, 591-598.
25. Yee, C. J.; DeFrances, M. C.; Bell, A.; Bowen, W.; Petersen, B.; Michalopoulos, G. K.; Zarnegar, R. Expression and characterization of biologically active human hepatocyte growth factor (HGF) by insect cells infected with HGF-recombinant baculovirus. Biochemistry 1993, 32, 7922-7931.
26. Naldini, L.; Tamagnone, L.; Vigna, E.; Sachs, M.; Hartmann, G.; Birchmeier, W.; Daikuhara, Y.; Tsubouchi, H.; Blasi, F.; Comoglio, P. M. Extacellular proteolytic cleavage by urokinase is required for activation of hepatocyte growth factor/scatter factor. EMBO J. 1992, 11, 4825-4833.
27. O'Reilly, D. R.; Miller, L. K.; Luckow, V. A. Baculovirus Expression Vectors: A Laboratory Manual; W. H. Freeman and Company: New York, 1992.
28. Ramabhadran, T. V.; Gandy, S. E.; Ghiso, J.; Czernik, A. J.; Ferris, D.; Bhasin R.; Goldgaber, D.; Frangione, B.; Greengard, P. Proteolytic processing of human amyloid β protein precursor in insect cells. J. Bio. Chem. 1993, 268, 2009-2012.
29. Pyle, L. E.; Barton, P.; Fujiwara, Y.; Mitchell, A.; Fidge, N. Secretion of biologically active human proapolipoprotein A-I in a baculovirus-insect cell system: protection form degradation by protease inhibitors. J. Lipid Res. 1995, 36, 2355-2361.
30. Slack, J. M.; Kuzio, J.; Faulkner, P. Characterization of v-cath, a cathepsin L-like proteinase expressed by the baculovirus Autographa californica nuclear polyhedrosis virus. J. Gen. Virol. 1995, 76, 1091-1098.
31. Grosch, H.; Hasilik, A. Protection of proteolysis-prone recombinant proteins in baculovirus expression systems. BioTechniques 1998, 24, 930-934.
32. Naggie, S.; Bentley, W. E. Appearance of protease activities coincides with p10 and polyhedrin-driven protein production in the baculovirus expression system: effects on yield. Biotechnol. Prog. 1998, 14, 227-232.
33. Jiang, B.; Barniak, V.; Smith, R. P.; Sharma, R.; Corsaro, B.; Hu, B.; Madore, H. P. Synthesis of rotavirus-like particles in insect cells: comparative and quantitative analysis. Biotechnol. Bioeng. 1998, 60, 369-374.
34. Shih, C. J.; Lin, R. W.; Wang, C. H. Establishment of a cell from Spodoptera litura (Lepidoptera: Noctuidae) and replication of S. litura nuclear polyhedrosis virus in vitro. J. Invertebr. Pathol. 1997, 69, 1-6.
35. Wang, M. Y.; Vakharia, V.; Bentley, W. E. Synthesis of epoxide hydroxylase: a focus on infected cell. Biotechnol. Bioeng. 1993, 42, 240-247.
36. Sambrook, J.; Fritsch, E. F.; Maniatis, T. Molecular Cloning: A Laboratory Manual, 2nd ed.; Cold Spring Harbor Laboratory Press: New York, 1989.
37. Lee, H. S.; Huang, G. T.; Sheu, J. C.; Chiou, L. L.; Horng, M. C.; Lai, M. Y.; Chen, D. S.; Lee, S. C. Lack of critical domains in the β-chain of hepatocyte growth factor. Biochem. Biophys. Res. Commun. 1995, 210, 1017-1024.
38. Vernet, T.; Tessier, D. C.; Richardson, C.; Lalibrte, F.; Khouri, H. E.; Bell, A. W.; Storer, A. C.; Thomas, D. Y. Secretion of functional papain precursor from insect cells. J. Biol. Chem. 1990, 265, 16661-16666.
39. Wang, M. Y.; Pulliam, T. R.; Valle, M.; Vakharia, V. N.; Bentley, W. E. Kinetic analysis of alkaline protease activity, recombinant protein production and metabolites for infected insect (Sf9) cells under different DO levels. J. Biotechnol. 1996, 46, 243-254.