pH driven conformational dynamics and dimer-to-monomer transition in DLC8

Protein Science

Volumn 15, Issue 2, 2006, Pages 335-342

  Mohan, P M Krishna ^a   Barve, Maneesha ^a   Chatterjee, Amarnath ^a   Hosur, Ramakrishna V ^a  

^a TATA INSTITUTE OF FUNDAMENTAL RESEARCH   (India)

Author keywords

15N transverse relaxation; Conformational exchange; Dimer monomer equilibrium; Dynein light chain protein; Line broadening; Nuclear magnetic resonance; pH driven conformational transitions

Indexed keywords

DYNEIN ADENOSINE TRIPHOSPHATASE;

ARTICLE; CONFORMATIONAL TRANSITION; DIMERIZATION; DISSOCIATION; HYDROGEN BOND; HYDROPHOBICITY; MOLECULAR DYNAMICS; NITROGEN NUCLEAR MAGNETIC RESONANCE; PH MEASUREMENT; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; PROTON TRANSPORT; STRUCTURE ANALYSIS;

ANIMALS; CARRIER PROTEINS; DIMERIZATION; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; DYNEIN ATPASE; HYDROGEN-ION CONCENTRATION; MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN BINDING; PROTEIN CONFORMATION; PROTONS;

EID: 31344449610     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.051854906     Document Type: Article

References (31)

1. Barbar, E. and Hare, M. 2004. Characterization of the cargo attachment complex of cytoplasmic dynein using NMR and mass spectrometry. Meth. Enzymol. 380: 219-241.
2. Barbar, E., Kleinman, B., Imhoff, D., Li, M., Hays, T.S., and Hare, M. 2001. Dimerization and folding of LC8, a highly conserved light chain of cytoplasmic dynein. Biochemistry 40: 1596-1605.
3. Day, C.L., Puthalakath, H., Skea, G., Strasser, A., Barsukov, I., Lian, L.Y., Huang, D.C., and Hinds, M.G. 2004. Localization of dynein light chains 1 and 2 and their pro-apoptotic ligands. Biochem. J. 377: 597-605.
4. Espindola, F.S., Suter, D.M., Partata, L.B., Cao, T., Wolenski, J.S., Cheney, R.E., King, S.M., and Mooseker, M.S. 2000. The light chain composition of chicken brain myosin-Va: Calmodulin, myosin-II essential light chains, and 8-kDa dynein light chain/PIN. Cell Motil. Cytoskeleton 47: 269-281.
5. Fan, J.S., Zhang, Q., Li, M., Tochio, H., Yamazaki, T., Shimizu, M., and Zhang, M. 1998. Protein inhibitor of neuronal nitric-oxide synthase, PIN, binds to a 17-amino acid residue fragment of the enzyme. J. Biol. Chem. 273: 33472-33481.
6. Fan, J., Zhang, Q., Tochio, H., Li, M., and Zhang, M. 2001. Structural basis of diverse sequence-dependent target recognition by the 8 kDa dynein light chain. J. Mol. Biol. 306: 97-108.
7. Fan, J.S., Zhang, Q., Tochio, H., and Zhang, M. 2002. Backbone dynamics of the 8 kDa dynein light chain dimer reveals molecular basis of the protein's functional diversity. J. Biomol. NMR 23: 103-114.
8. Farrow, N.A., Muhandiram, R., Singer, A.U., Pascal, S.M., Kay, C.M., Gish, G., Shoelson, S.E., Pawson, T., Forman-Kay, J.D., and Kay, L.E. 1994. Backbone dynamics of a free and phosphopeptide-complexed Src homology 2 domain studied by 15N NMR relaxation. Biochemistry 33: 5984-6003.
9. Fasman, G.D. 1989. Practical handbook of biochemistry and molecular biology. CRC Press, Boca Raton, Florida.
10. Ferentz, A.E. and Wagner, G. 2000. NMR spectroscopy: A multifaceted approach to macromolecular structure. Q. Rev. Biophys. 33: 29-65.
11. Fuhrmann, J.C., Kins, S., Rostaing, P., El Far, O., Kirsch, J., Sheng, M., Triller, A., Betz, H., and Kneussel, M. 2002. Gephyrin interacts with Dynein light chains 1 and 2, components of motor protein complexes. J. Neurosci. 22: 5393-5402.
12. Harrison, A. and King, S.M. 2000. The molecular anatomy of dynein. Essays Biochem. 35: 75-87.
13. Jaffrey, S.R. and Snyder, S.H. 1996. PIN: An associated protein inhibitor of neuronal nitric oxide synthase. Science 274: 774-777.
14. King, S.M. 2000. The dynein microtubule motor. Biochim. Biophys. Acta 1496: 60-75.
15. King, S.M. and Patel-King, R.S. 1995. The M(r) = 8,000 and 11,000 outer arm dynein light chains from Chlamydomonas flagella have cytoplasmic homologues. J. Biol. Chem. 270: 11445-11452.
16. King, S.M., Barbarese, E., Dillman III, J.F., Benashski, S.E., Do, K.T., Patel-King, R.S., and Pfister, K.K. 1998. Cytoplasmic dynein contains a family of differentially expressed light chains. Biochemistry 37: 15033-15041.
17. Koonce, M.P. and Samso, M. 2004. Of rings and levers: The dynein motor comes of age. Trends Cell Biol. 14: 612-619.
18. Liang, J., Jaffrey, S.R., Guo, W., Snyder, S.H., and Clardy, J. 1999. Structure of the PIN/LC8 dimer with a bound peptide. Nat. Struct. Biol. 6: 735-740.
19. Lo, K.W., Naisbitt, S., Fan, J.S., Sheng, M., and Zhang, M. 2001. The 8-kDa dynein light chain binds to its targets via a conserved (K/R)XTQT motif. J. Biol. Chem. 276: 14059-14066.
20. Lo, K.W., Kan, H.M., Chan, L.N., Xu, W.G., Wang, K.P., Wu, Z., Sheng, M., and Zhang, M. 2005. The 8-kDa dynein light chain binds to p53-binding protein 1 and mediates DNA damage-induced p53 nuclear accumulation. J. Biol. Chem. 280: 8172-8179.
21. Makokha, M., Huang, Y.J., Montelione, G., Edison, A.S., and Barbar, E. 2004. The solution structure of the pH-induced monomer of dynein light-chain LC8 from Drosophila. Protein Sci. 13: 727-734.
22. Naisbitt, S., Valtschanoff, J., Allison, D.W., Sala, C., Kim, E., Craig, A.M., Weinberg, R.J., and Sheng, M. 2000. Interaction of the postsynaptic density-95/guanylate kinase domain-associated protein complex with a light chain of myosin-V and dynein. J. Neurosci. 20: 4524-4534.
23. Nyarko, A., Cochrun, L., Norwood, S., Pursifull, N., Voth, A., and Barbar, E. 2005. Ionization of His 55 at the dimer interface of dynein light-chain LC8 is coupled to dimer dissociation. Biochemistry 44: 14248-14255.
24. Panchal, S.C., Bhavesh, N.S., and Hosur, R.V. 2001. Improved 3D triple resonance experiments, HNN and HN(C)N, for HN and 15N sequential correlations in (13C, 15N) labeled proteins: Application to unfolded proteins. J. Biomol. NMR 20: 135-147.
25. Pfister, K.K. 2005. Dynein cargo gets its groove back. Structure (Camb.) 13: 172-173.
26. Puthalakath, H., Huang, D.C., O'Reilly, L.A., King, S.M., and Strasser, A. 1999. The proapoptotic activity of the Bcl-2 family member Bim is regulated by interaction with the dynein motor complex. Mol. Cell 3: 287-296.
27. Puthalakath, H., Villunger, A., O'Reilly, L.A., Beaumont, J.G., Coultas, L., Cheney, R.E., Huang, D.C., and Strasser, A. 2001. Bmf: A proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis. Science 293: 1829-1832.
28. Schnorrer, F., Bohmann, K., and Nusslein-Volhard, C. 2000. The molecular motor dynein is involved in targeting swallow and bicoid RNA to the anterior pole of Drosophila oocytes. Nat. Cell Biol. 2: 185-190.
29. Vadlamudi, R.K., Bagheri-Yarmand, R., Yang, Z., Balasenthil, S., Nguyen, D., Sahin, A.A., den Hollander, P., and Kumar, R. 2004. Dynein light chain 1, a p21-activated kinase 1-interacting substrate, promotes cancerous phenotypes. Cancer Cell 5: 575-585.
30. Wang, W., Lo, K.W., Kan, H.M., Fan, J.S., and Zhang, M. 2003. Structure of the monomeric 8-kDa dynein light chain and mechanism of the domain-swapped dimer assembly. J. Biol. Chem. 278: 41491-41499.
31. Yang, Z., Vadlamudi, R.K., and Kumar, R. 2005. Dynein light chain 1 phosphorylation controls macropinocytosis. J. Biol. Chem. 280: 654-659.