In vitro studies of the genotoxicity of ionizing radiation in human G 0 T lymphocytes

Environmental and Molecular Mutagenesis

Volumn 46, Issue 4, 2005, Pages 207-220

  O'Neill, Patrick ^a   Nicklas, Janice ^a   Hirsch, Betsy ^b   Jostes, Rick ^c   Hunter, Timothy ^a   Sullivan, Linda ^a   Albertini, Richard ^a  

^a UNIVERSITY OF VERMONT   (United States)

^b UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

^c NATIONAL ACADEMIES OF SCIENCES   (United States)

Author keywords

HPRT mutation; Human mutation; Ionizing radiation; T lymphocyte; Translocation

Indexed keywords

CESIUM 137; CYTARABINE; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; RADON 222;

ARTICLE; CELL CYCLE G0 PHASE; CHROMOSOME PAINTING; CONTROLLED STUDY; DNA REPAIR; DNA SEQUENCE; GENE; GENE DELETION; GENE MUTATION; GENOTOXICITY; HPRT GENE; HUMAN; HUMAN CELL; IN VITRO STUDY; IONIZING RADIATION; NUCLEIC ACID BASE SUBSTITUTION; RADIATION DOSE; RADIATION EXPOSURE; T LYMPHOCYTE;

CELL DIVISION; CELL PROLIFERATION; CELLS, CULTURED; CESIUM RADIOISOTOPES; DOSE-RESPONSE RELATIONSHIP, RADIATION; G0 PHASE; HUMANS; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; LINEAR ENERGY TRANSFER; MUTAGENICITY TESTS; MUTATION; RADIATION, IONIZING; RADON; T-LYMPHOCYTES;

EID: 30844441376     PISSN: 08936692     EISSN: 10982280     Source Type: Journal    
DOI: 10.1002/em.20143     Document Type: Article

References (61)

1. Albertini RJ, Castle KL, Borcherding WR. 1982. T-cell cloning to detect the mutant 6-thioguanine resistant lymphocytes present in human peripheral blood. Proc. Natl. Acad. Sci. USA 79:6617-6621.
2. Albertini RJ, O'Neill JP, Nicklas JA, Heintz NH, Kelleher PC. 1985. Alterations of the HPRT gene in human 6-thioguanine resistant T-lymphocytes arising in vivo. Nature 316:369-371.
3. Albertini RJ, Clark LS, Nicklas JA, O'Neill JP, Hui TE, Jostes RF. 1997. Radiation quality affects efficacy of induction and molecular spectrum of HPRT mutations in human T-cells. Radiat Res 148:576-86.
4. Albertini RJ, Nicklas JA, Skopek TR, Recio L, O'Neill JP. 1998. Genetic instability in human T-lymphocytes. Mutat Res 400:381-389.
5. Amundson SA, Do KT, Shahab S, Bittner M, Meltzer P, Trent J, Fornace AJ, Jr. 2000. Identification of potential mRNA biomarkers in peripheral blood lymphocytes for human exposure to ionizing radiation. Radiat Res 154:342-346.
6. Amundson SA, Fornace AJ, Jr. 2003. Monitoring human radiation exposure by gene expression profiling: possibilities and pitfalls. Health Phys 85:36-42.
7. Anderson RM, Stevens DL, Goodhead DT. 2002. M-FISH analysis shows that complex chromosome aberrations induced by alpha particle tracks are cumulative products of localized rearrangements. Proc Natl Acad Sci USA 99:12167-12172.
8. Bao C-Y, Ma A-H, Evans HH, Horng MF, Mencl J, Hui TE, Sedwick WD. 1995. Molecular analysis of HPRT deletions induced by α and X-irradiation in human lymphoblastoid cells. Mutat Res 326:1-15.
9. Bender MA, Gooch PC. 1962. Types and rates of X-ray induced chromosome aberrations in human blood irradiated in vitro. Proc Natl Acad Sci (USA) 48:522-532.
10. Burki HJ. 1980. Ionizing radiation-induced 6-thioguanine resistant clones in synchronous CHO cells. Radiat Res 81:76-84.
11. Clark LS, Albertini RJ, Nicklas JA. 1997. The aminothiol WR-1065 protects T lymphocytes from ionizing radiation-induced deletions of the HPRT gene. Cancer Epidemiol Biomarkers Prev 6:1033-1037.
12. Cole J, Skopek TR. 1994. Somatic mutant frequency, mutation rates and mutation spectra in the human population in vivo. Mutat Res 304:33-106.
13. Cox R, Masson WK. 1978. Do radiation induced thioguanine resistant mutants of cultured mammalian cells arise by HPRT gene mutation or X chromosome rearrangement? Nature 276:629-630.
14. Curry J, Karnaoukhova L, Guenette GC, Glickman BW. 1999. Influence of sex, smoking and age on human HPRT mutation frequencies and spectra. Genetics 152:1065-1077.
15. Finette BA, Sullivan LM, O'Neill JP, Nicklas JA, Vacek PM, Albertini RJ. 1994. Determination of HPRT mutant frequencies in T-lymphocytes from a healthy pediatric population: statistical comparison between newborn, children and adult mutant frequencies, cloning efficiency and age. Mutat Res 308:223-231.
16. Finette BA, Homans AC, Albertini RJ. 2000. Emergence of genetic instability in children treated for leukemia. Science 288:514-517.
17. Fink JM, Dobyns WB, Guerrini R, Hirsch BA. 1997. Identification of a duplication of Xq28 associated with bilateral periventricular nodular heterotopia. Am J Hum Genet 61:379-387.
18. Gibbs RA, Nguyen PN, McBride LJ, Koepf SM, Caskey CT. 1989. Identification of mutations leading to the Lesch-Nyhan syndrome by automated direct DNA sequencing of in vitro amplified cDNA. Proc Natl Acad Sci USA 86:1919-1923.
19. Gibbs RA, Nguyen PN, Edwards A, Civitello AB, Caskey CT. 1990. Multiplex DNA deletion detection and exon sequencing of the hypoxanthine phosphoribosyltransferase gene in Lesch-Nyhan families. Genomics 7:235-244.
20. Heartlein MW, Preston RJ. 1985. An explanation of interspecific differences in sensitivity to X ray induced chromosome aberrations and a consideration of dose response curves. Mutat Res 150:299-305.
21. Hiss EA, Preston RJ. 1977. The effect of cytosine arabinoside on the frequency of single strand breaks in mammalian cells following irradiation or chemical treatment. Biochim Biophys Acta 478:1-8.
22. Inoue M, Shen GP, Chaudhry MA, Galick H, Blaisdell JO, Wallace SS. 2004. Expression of the oxidative base excision repair enzymes is not induced in TK6 human lymphoblastoid cells after low doses of ionizing radiation. Radiat Res 161:409-417.
23. Jones IM, Thomas CB, Haag K, Pleshanov P, Vorobstova I, Tureva L, Nelson DO. 1999. Total gene deletions and mutant frequency of the HPRT gene as indicators of radiation exposure in Chernobyl liquidators. Mutat Res 431:233-246.
24. Jostes RF, Bushness KM, Dewey WC. 1980. X-ray induction of 8-aza-guanine resistant mutants in synchronous Chinese hamster ovary cells. Radiat Res 83:146-461.
25. Jostes RF, Hui TE, James AC, Cross FT, Schwartz JL, Rotmensch J, Atcher RW, Evans HH, Mencl J, Bakale G, Rao PS. 1991. In vitro exposure of mammalian cells to radon: dosimetric considerations. Radiat Res 127:211-219.
26. Kodama Y, Hakoda M, Shimba H, Awa AA, Akiyama M. 1989. A chromosome study of 6-thioguanine-resistant mutants in T lymphocytes of Hiroshima atomic bomb survivors. Mutat Res 227:31-38.
27. Lengauer C, Green ED, Cremer T. 1992. Fluorescence in situ hybridization of YAC clones after Alu-PCR amplification. Genomics 13:826-828.
28. Lippert MJ, Albertini RJ, Nicklas JA. 1995a. Physical mapping of the HPRT chromosomal region (Xq26). Mutat Res 326:39-49.
29. Lippert MJ, Nicklas JA, Hunter TC, Albertini RJ. 1995b. Pulsed field analysis of HPRT Tcell large deletions: telomeric region breakpoint spectrum. Mutat Res 326:51-64.
30. Mayer C, Popanda O, Zelezny O, von Brevern M-C, Bach A, Bartsch H, Schmezer P. 2002. DNA repair capacity after gamma-irradiation and expression profiles of DNA repair genes in resting and proliferating human peripheral blood lymphocytes. DNA Repair 1:237-250.
31. 0 phase lymphocytes irradiated in vitro with gamma rays. Mutat Res 474:147-158.
32. 0 lymphocytes with low energy protons (28 keV/microm): HPRT mutations and chromosome aberrations. Radiat Res 160:52-60.
33. Morley AA, Trainor KJ, Seshadri R, Ryall RG. 1983. Measurement of in vivo mutations in human lymphocytes. Nature 302:155-156.
34. Nicklas JA, O'Neill JP, Albertini RJ. 1986. Use of T-cell receptor probes to quantify the in vivo HPRT mutations in human lymphocytes. Mutat Res 173:65-72.
35. Nicklas JA, Hunter TC, Sullivan LM, Berman JK, O'Neill JP, Albertini RJ. 1987. Molecular analyses of in vivo hprt mutations in human T-lymphocytes. I. Studies of low frequency "spontaneous" mutants by Southern blots Mutagen 2:341-347.
36. Nicklas JA, O'Neill JP, Sullivan LM, Hunter TC, Allegretta M, Chastenay B, Libbus BL, Albertini RJ. 1988. Molecular analysis of in vivo HPRT mutations in human T-lymphocytes: II. Demonstration of a clonal amplification of HPRT mutant T-lymphocytes in vivo. Environ Mol Mutagen 12:271-284.
37. Nicklas JA, Hunter TC, O'Neill JP, Albertini RJ. 1989. Molecular analyses of in vivo hprt mutations in human T-lymphocytes: III. Longitudinal study of HPRT gene structural alterations and T-cell clonal origins. Mutat Res 215:147-160.
38. Nicklas JA, Falta MT, Hunter TC, O'Neill JP, Jacobson-Kram D, Williams J, Albertini RJ. 1990a. Molecular analysis of in vivo HPRT mutations in human lymphocytes V. Effects of total body irradiation secondary to radioimmunoglobulin therapy (RIT). Mutagen 5:461-468.
39. Nicklas JA, Hunter TC, O'Neill JP, ALbertini RJ. 1990b. Fine structure mapping of the HPRT gene region of the human X chromosome (Xq26). Am J Hum Genet 49:267-278.
40. Nicklas JA, O'Neill JP, Hunter TC, Falta MT, Lippert MJ, Jacobson-Kram D, Williams JR, Albertini RJ. 1991. In vivo ionizing irradiations produce deletions in the HPRT gene of human T-lymphocytes. Mutat Res 250:383-396.
41. 1 phase Chinese hamster ovary cells. Mutat Res 150:443-450.
42. O'Neill JP, McGinniss MJ, Bennan JK, Sullivan LM, Nicklas JA, Albertini RJ. 1987. Refinement of a T-lymphocyte cloning assay to quantify the in vivo thioguanine-resistant mutant frequency in humans. Mutagenesis 2:87-94.
43. O'Neill JP, Sullivan LM, Albertini RJ. 1990a. In vitro induction, expression and selection of thioguanine resistant mutants with human T-lymphocytes. Mutat Res 240:135-142.
44. O'Neill JP, Hunter TC, Sullivan LM, Nicklas JA, Albertini RJ. 1990b. Southern blot analyses of human T-lymphocyte mutants induced in vitro by gamma irradiation. Mutat Res 240:143-149.
45. O'Neill JP, Sullivan LM, Hunter TC, Nicklas, JA. 1991. Cytosine arabinoside enhancement of gamma irradiation induced mutations in human T-lymphocytes. Environ Mol Mutagen 17:231-237.
46. O'Neill JP, Nicklas JA, Hunter TC, Batson OB, Allegretta M, Falta MT, Branda RF, Albertini RJ. 1994. The effect of T-lymphocyte clonality on the calculated HPRT mutation frequency occurring in vivo in humans. Mutat Res 313:215-225.
47. Preston RJ. 1980. The effect of cytosine arabinoside on the frequency of X ray induced chromosome aberrations in normal human leukocytes. Mutat Res 69:71-79.
48. Preston RJ. 1990. Mechanisms of Induction of Specific Chromosomal Alterations. DNA Damage and Repair in Human Tissues. New York: Plenum Press. pp 329-336.
49. Rainville IR, Albertini RJ, Nicklas JA. 1995. Breakpoints and junctional regions of intragenic deletions in the HPRT gene in human T cells. Somat Cell Mol Genet 21:309-326.
50. Robinson D, Goodall K, Albertini RJ, O'Neill JP, Finnette F, Sala-Trepat E, Moustacchi E, Tates AD, Beare DM, Green MHL, Cole J. 1994. An analysis of in vivo HPRT mutant frequency in circulating T-lymphocytes in a normal human population: a comparison of four databases. Mutat Res 313:227-247.
51. Ruttenber AJ, Harrison LT, Baron A, McClure D, Glanz J, Quillin R, O'Neill JP, Sullivan LM, Campbell J, Nicklas JA. 2001. HPRT mutant frequencies, non-pulmonary malignancies and domestic radon exposure: Postmortum analysis of an interesting hypothesis. Environ Mol Mutagen 37:7-16.
52. Sankaranarayanan K. 1982. Genetic Effects of Ionizing Radiation in Multicellular Eukaryotes and the Assessment of Genetic Radiation Hazards in Man. Amsterdam: Elsevier.
53. Sankaranarayanan K, Chakraborty R. 2000. Ionizing radiation and genetic risks. 2000. XIII. Summary and synthesis of papers VI to XII, estimates of genetic risks in the year 2000. Mutat Res 453:183-197.
54. Schroder H, Heimers A. 2002. Chromosome aberrations induced in human lymphocytes by in vitro and in vivo X-rays. Mutat Res 517:167-172.
55. Simpson P, Morris T, Savage J, Thacker J. 1993. High-resolution cytogenetic analysis of X-ray induced mutations of the HPRT gene of primary human fibroblasts. Cytogenet Cell Genet 64:39-45.
56. Singleton BK, Griffin CS, Thacker J. 2002. Clustered DNA damage leads to complex genetic changes in irradiated human cells. Cancer Res 62:6263-6269.
57. Strauss GS, Albertini RJ. 1979. Enumeration of 6-thioguanine resistant peripheral blood lymphocytes in man as a potential test for somatic cell mutations arising in vivo. Mutat Res 61:353-379.
58. Thacker J, Stretch A. 1983. Recovery from lethal and mutagenic damage during post-irradiation holding and low dose rate irradiations of cultured hamster cells. Radiat Res 96:380-392.
59. Thomas CB, Nelson DO, Pleshanov P, Jones IR. 2002. Induction and decline of HPRT mutants and deletions following a low dose radiation exposure at Chernobyl. Mutat Res 499:177-187.
60. Williams M, Rainville IR, Nicklas JA. 2002. Use of inverse PCR to amplify and sequence breakpoints of HPRT deletion and translocation mutations. Environ Mol Mutagen 39:22-32.
61. Yamada Y, Park MS, Okinaka RT, Chen DJ. 1996. Molecular analysis and comparison of radiation-induced large deletions of the HPRT locus in primary human skin fibroblasts. Radiat Res 145(4):481-490.