Gastroprotective activity of Nigella sativa L oil and its constituent, thymoquinone against acute alcohol-induced gastric mucosal injury in rats

World Journal of Gastroenterology

Volumn 11, Issue 42, 2005, Pages 6662-6666

  Kanter, Mehmet ^a   Demir, Halit ^b   Karakaya, Cengiz ^b   Ozbek, Hanefi ^b  

^a TRAKYA UNIVERSITY   (Turkey)

^b YUZUNCU YIL UNIVERSITY   (Turkey)

Author keywords

Anti oxidant; Nigella sativa; Rat; Thymoquinone; Ulcer

Indexed keywords

ALCOHOL; CATALASE; GLUTATHIONE; GLUTATHIONE TRANSFERASE; MALONALDEHYDE; NIGELLA SATIVA EXTRACT; REACTIVE OXYGEN METABOLITE; SODIUM CHLORIDE; SUPEROXIDE DISMUTASE; THIOBARBITURIC ACID REACTIVE SUBSTANCE; THYMOQUINONE;

ALCOHOL CONSUMPTION; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIOXIDANT ACTIVITY; ARTICLE; CONTROLLED STUDY; DRUG MECHANISM; ENZYME ACTIVITY; LIPID PEROXIDATION; MALE; NIGELLA; NONHUMAN; RAT; STOMACH MUCOSA INJURY; STOMACH PROTECTION; ULCER HEALING;

EID: 30444448245     PISSN: 10079327     EISSN: None     Source Type: Journal    
DOI: 10.3748/wjg.v11.i42.6662     Document Type: Article

References (37)

1. Szabo S, Trier JS, Frankel PW. Sulfhydryl compounds may mediate gastric cytoprotection. Science 1981; 214: 200-202
2. Cho CH, Pfeiffer CJ, Misra HP. Ulcerogenic mechanism of ethanol and the action of sulphanilyl fluoride on the rat stomach in-vivo. J Pharm Pharmacol 1991; 43: 495-498
3. Lutnicki K, Wrobel J, Ledwozyw A, Trebas-Pietras E. The effect of calcium ions on the intensity of peroxidation processes and the severity of ethanol-induced injury to the rat's gastric mucosa. Arch Vet Pol 1992; 32: 125-132
4. Bast A, Haenen GR, Doelman CJ. Oxidants and antioxidants: state of the art. Am J Med 1991; 91: 2S-13S
5. Hirokawa M, Miura S, Yoshida H, Kurose I, Shigematsu T, Hokari R, Higuchi H, Watanabe N, Yokoyama Y, Kimura H, Kato S, Ishii H. Oxidative stress and mitochondrial damage precedes gastric mucosal cell death induced by ethanol administration. Alcohol Clin Exp Res 1998; 22: 111S-114S
6. La Casa C, Villegas I, Alarcon de la Lastra C, Motilva V, Martin Calero MJ. Evidence for protective and antioxidant properties of rutin, a natural flavone, against ethanol induced gastric lesions. J Ethnopharmocal 2000; 71: 45-53
7. el Tahir KE, Ashour MM, al-Harbi MM. The respiratory effects of the volatile oil of the black seed (Nigella sativa) in guinea-pigs: elucidation of the mechanism(s) of action. Gen Pharmacol 1993; 24: 1115-1122
8. El-Kadi A, Kandil O. The black seed (Nigella sativa) and immunity: its effect on human T cell subset. Fed Proc 1987; 46: 1222
9. Hanafy MS, Hatem ME. Studies on the antimicrobial activity of Nigella sativa seed (black cumin). J Ethnopharmacol 1991; 34: 275-278
10. Zaoui A, Cherrah Y, Lacaille-Dubois MA, Settaf A, Amarouch H, Hassar M. Diuretic and hypotensive effects of Nigella sativa in the spontaneously hypertensive rat. Therapie 2000; 55:379-382
11. Turkdogan MK, Agaoglu Z, Yener Z, Sekeroglu R, Akkan HA, Avci ME. The role of antioxidant vitamins (C and E), selenium and Nigella sativa in the prevention of liver fibrosis and cirrhosis in rabbits: new hopes. Dtsch Tierarztl Wochenschr 2001; 108: 71-73
12. Kanter M, Meral I, Yener Z, Ozbek H, Demir H. Partial regeneration/proliferation of the beta-cells in the islets of Langerhans by Nigella sativa L. in streptozotocin-induced diabetic rats. Tohoku J Exp Med 2003; 201: 213-219
13. Houghton PJ, Zarka R, de las Heras B, Hoult JR. Fixed oil of Nigella sativa and derived thymoquinone inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Med 1995; 61: 33-36
14. Ohkawa H, Ohishi N, Yagi K. Reaction of linoleic acid hydroperoxide with thiobarbituric acid. J Lipid Res 1978; 19: 1053-1057
15. Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959; 82: 214-226
16. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34: 497-500
17. Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974; 249: 7130-7139
18. Clairborne A. Catalase activity. In: Greenwald RA, editor. Handbook of Methods for Oxygen Radical Research. Boca Raton, FL 1985; 383-384
19. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265-275
20. Halliwell B, Gutteridge JM, Cross CE. Free radicals, antioxidants, and human disease: where are we now? J Lab Clin Med 1992; 119: 598-620
21. El-Habit OH, Saada HN, Azab KS, Abdel-Rahman M, El-Malah DF. The modifying effect of beta-carotene on gamma radiation-induced elevation of oxidative reactions and genotoxicity in male rats. Mutat Res 2000; 466: 179-186
22. Tuncel N, Erkasap N, Sahinturk V, Ak DD, Tuncel M. The protective effect of vasoactive intestinal peptide (VIP) on stress-induced gastric ulceration in rats. Ann N Y Acad Sci 1998; 865: 309-322
23. Suzuki Y, Ishihara M, Segami T, Ito M. Anti-ulcer effects of antioxidants, quercetin, alpha-tocopherol, nifedipine and tetracycline in rats. Jpn J Pharmacol 1998; 78: 435-441
24. Brzozowski T, Konturek PC, Konturek SJ, Pajdo R, Bielanski W, Brzozowska I, Stachura J, Hahn EG. The role of melatonin and L-tryptophan in prevention of acute gastric lesions induced by stress, ethanol, ischemia, and aspirin. J Pineal Res 1997; 23: 79-89
25. Alarcon de la Lastra C, Motilva V, Martin MJ, Nieto A, Barranco MD, Cabeza J, Herrerias JM. Protective effect of melatonin on indomethacin-induced gastric injury in rats. J Pineal Res 1999; 26: 101-107
26. Bilici D, Suleyman H, Banoglu ZN, Kiziltunc A, Avci B, Ciftcioglu A, Bilici S. Melatonin prevents ethanol-induced gastric mucosal damage possibly due to its antioxidant effect. Dig Dis Sci 2002; 47: 856-861
27. El-Missiry MA, El-Sayed IH, Othman AI. Protection by metal complexes with SOD-mimetic activity against oxidative gastric injury induced by indomethacin and ethanol in rats. Ann Clin Biochem 2001; 38: 694-700
28. Kato S, Kitamura M, Korolkiewicz RP, Takeuchi K. Role of nitric oxide in regulation of gastric acid secretion in rats: effects of NO donors and NO synthase inhibitor. Br J Pharmacol 1998; 123: 839-846
29. Holzer P, Livingston EH, Saria A, Guth PH. Sensory neurons mediate protective vasodilatation in rat gastric mucosa. Am J Physiol 1981; 260: 363-370
30. Ko JK, Cho CH, Ogle CW. The vagus nerve and its non-cholinergic mechanism in the modulation of ethanol-induced gastric mucosal damage in rats. J Pharm Pharmacol 1994; 46: 29-33
31. Cho CH, Pfeiffer CJ, Misra HP. Ethanol and the antioxidant defense in the gastrointestinal tract. Acta Physiol Hung 1992; 80: 99-105
32. Ligumsky M, Sestieri M, Okon E, Ginsburg I. Antioxidants inhibit ethanol-induced gastric injury in the rat. Role of manganese, glycine, and carotene. Scand J Gastroenterol 1995; 30: 854-860
33. El-Dakhakhny M, Barakat M, El-Halim MA, Aly SM. Effects of Nigella sativa oil on gastric secretion and ethanol induced ulcer in rats. J Ethnopharmacol 2000; 72: 299-304
34. Saleh S, El-Denshary EMS, Mahran LG, Salah N. Anti-inflammatory and antioxidant effect of Nigella sativa oil in sensitized animals. The 25th International Conference on Science and Technology, New Delhi 2000
35. Campbell WB, Halushka PV. Lipid-derived autacoids. Eicosanoids and platelet-activating factors. In: Hardman JG, Limbird LE, Molinoff PB, Ruddon RW, Gilman AG. Goodman and Gilman's. The Pharmacol Basis of Therapeutics, McGraw-Hill, New York 1996; 601-616
36. Tsuji S, Kawano S, Sato N, Kamada T. Mucosal blood flow stasis and hypoxemia as the pathogenesis of acute gastric mucosal injury: role of endogenous leukotrienes and prostaglandins. J Clin Gastroenterol 1990; 12 Suppl 1: S85-S91
37. Mansour MA. Protective effects of thymoquinone and desferrioxamine against hepatotoxicity of carbon tetrachloride in mice. Life Sci 2000; 66: 2583-2591