Imaging enzyme activity with polarization-sensitive confocal fluorescence microscopy

Journal of Microscopy

Volumn 215, Issue 1, 2004, Pages 24-33

  Bigelow, Chad E ^b   Vishwasrao, Harshad D ^d   Frelinger, John G ^c   Foster, Thomas H ^a,b  

^a UNIVERSITY OF ROCHESTER   (United States)

^b UNIVERSITY OF ROCHESTER   (United States)

^c UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

^d School of Operations Research and Industrial Engineering   (United States)

Author keywords

Confocal microscopy; Enzyme; Fluorescence; Polarization

Indexed keywords

ANISOTROPY; BODY FLUIDS; FLUORESCENCE IMAGING; FLUORESCENCE MICROSCOPY; POLARIZATION;

ANISOTROPY DECAY; BOVINE SERUM ALBUMINS; CONFOCAL FLUORESCENCE MICROSCOPY; CONFOCAL MICROSCOPES; ENZYMATIC ACTION; ENZYMES ACTIVITY; FLUORESCENCE ANISOTROPY MEASUREMENTS; POLARIZATION SENSITIVE; PROTEINASE K; STEADY-STATE FLUORESCENCE;

ENZYME ACTIVITY;

PROTEINASE K; TRYPSIN;

ANISOTROPY; ARTICLE; CONFOCAL MICROSCOPY; CORRELATION ANALYSIS; ENZYME ACTIVITY; ENZYME KINETICS; FLUORESCENCE MICROSCOPY; POLARIZATION; PRIORITY JOURNAL; STEADY STATE;

BOVINAE;

EID: 3042833837     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.0022-2720.2004.01357.x     Document Type: Article

References (27)

1. Axelrod, D. (1979) Carbocyanine dye orientation in red cell membrane studied by microscopic fluorescence polarization. Biophys. J. 26, 557-573.
2. Axelrod, D. (1989) Fluorescence polarization microscopy. Methods Cell Biol. 30, 333-352.
3. Bergström, F., Mikhalyov, I., Hägglöf, P., Wortmann, R., Ny, T. & Johansson, L.B.Å. (2002) Dimers of dipyrrometheneboron difluoride (BODIPY) with light spectroscopic applications in chemistry and biology. J. Am. Chem. Soc. 124, 196-204.
4. Bigelow, C.E., Conover, D.L. & Foster, T.H. (2003) Confocal fluorescence spectroscopy and anisotropy imaging system. Opt. Lett. 28, 695-697.
5. Bigelow, C.E., Harkrider, C.J., Conover, D.L., Foster, T.H., Georgakoudi, I., Mitra. S., Nichols, M.G. & Rajadhyaksha, M. (2001) Retrofitted confocal laser scanner for a commercial inverted fluorescence microscope. Rev. Sci. Instrum. 72, 3407-3410.
6. Blackman, S.M., Cobb, C.E., Beth, A.H. & Piston, D.W. (1996) The orientation of eosin-5-maleimide on human erythrocyte band 3 measured by fluorescence polarization microscopy. Biophys. J. 71, 194-208.
7. Bolger, R. & Checovich, W. (1994) A new protease activity assay using fluorescence polarization. Biotechniques, 17, 585-589.
8. Bremer, C., Bredow, S., Mahmood, U., Weissleder, R. & Tung, C.H. (2001) Optical imaging of matrix metalloproteinase-2 activity in tumors: feasibility study in a mouse model. Radiology, 221, 523-529.
9. Coussens, L.M., Fingleton, B. & Matrisia, L.M. (2002) Matrix metalloproteinase inhibitors and cancer: trials and tribulations. Science, 295, 2387-2392.
10. Dix, J.A. & Verkman, A.S. (1990) Mapping of fluorescence anisotropy in living cells by ratio imaging - application to cytoplasmic viscosity. Biophys. J. 57, 231-240.
11. Ebeling, W., Hennrich, N., Klockow, M., Metz, H., Orth, H.D. & Lang, H. (1974) Proteinase K from Tritirachium album Limber. Eur. J. Biochem. 47, 91-97.
12. French, T., So, P.T.C., Weaver, D.J., Coelho-Sampaio, T., Gratton, E., Voss, E.W. & Carrero, J. (1997) Two-photon fluorescence lifetime imaging microscopy of macrophage-mediated antigen processing. J. Microsc. 185, 339-353.
13. Gough, A.H. & Taylor, D.L. (1993) Fluorescence anisotropy imaging microscopy maps calmodulin-binding during cellular contraction and locomotion. J. Cell Biol. 121, 1095-1107.
14. Heikal, A.A., Hess, S.T. & Webb, W.W. (2001) Multiphoton molecular spectroscopy and excited state dynamics of enhanced green fluorescent protein (EGFP): acid-base specificity. Chem. Phys. 274, 37-55.
15. Johnson, I.D., Kang, H.C. & Haugland, R.P. (1991) Fluorescent membrane probes incorporating dipyrrometheneboron difluoride fluorophores. Anal. Biochem. 198, 228-237.
16. Lakowicz, J.R. (1999) Principles of Fluorescence Spectroscopy. Kluwer Academic, New York.
17. Maeda, H. (1979) Assay of proteolytic-enzymes by the fluorescence polarization technique. Anal. Biochem. 92, 222-227.
18. McIntyre, J.O., Fingleton, B., Wells, K.S., Piston, D.W., Lynch, C.C., Guatam, S. & Matrisian, L.M. (2004) Development of a novel fluorogenic proteolytic beacon for in vivo detection and imaging of tumour-associated matrix metalloproteinase-7 activity. Biochem. J. 377, 617-628.
19. Mikhalyov, I., Gretskaya, N., Bergström, F. & Johansson, L.B.Å. (2002) Electronic ground and excited state properties of dipyrrometheneboron difluoride (BODIPY): dimers with application to biosciences. Phys. Chem. Chem. Phys. 4, 5663-5670.
20. O'Connor, D.V. & Phillips, D. (1984) Time-Correlated Single Photon Counting. Academic Press, London.
21. Rao, J.S. (2003) Molecular mechanisms of glioma invasiveness: the role of proteases. Nat. Rev. Cancer, 3, 489-501.
22. Schade, S.Z., Jolley, M.E., Sarauer, B.J. & Simonson, L.G. (1996) BODIPY-alpha-casein, a pH-independent protein substrate for protease assays using fluorescence polarization. Anal. Biochem. 243, 1-7.
23. Smalyukh, I.I., Shiyanovskii, S.V. & Lavrentovich, O.D. (2001) Three-dimensional imaging of orientational order by fluorescence confocal polarizing microscopy. Chem. Phys. Lett. 336, 88-96.
24. Tung, C.H., Mahmood, U., Bredow, S. & Weissleder, R. (2000) In vivo imaging of proteolytic enzyme activity using a novel molecular reporter. Cancer Res. 60, 4953-4958.
25. Voet, D. & Voet, J.G. (1995) Biochemistry. John Wiley & Sons, New York.
26. Weaver, D.J., Durack, G. & Voss, E.W. (1997) Analysis of the intracellular processing of proteins; application of fluorescence polarization and a novel fluorescent probe. Cytometry, 28, 25-35.
27. Weissleder, R., Tung, C.H., Mahmood, U. & Bogdanov, A. (1999) In vivo imaging of tumors with protease-activated near-infrared fluorescent probes. Nat. Biotechnol. 17, 375-378.